Skip to main content
SpringerLink
Log in

Tomato leaf curl Bangalore virus (ToLCBV): infectivity and enhanced pathogenicity with diverse betasatellites

  • Brief Report
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

Genomic components of a begomovirus isolated from tomato plants showing leaf curl and stunting symptoms in farmer’s fields at Hessarghatta village near Bangalore, India, were cloned by rolling-circle amplification. The virus was identified as a variant of strain C of the species Tomato leaf curl Bangalore virus and designated as Tomato leaf curl Bangalore virus-C[India:Hessarghatta:2008], ToLCBV-C[IN:Hess:08]. The betasatellite isolated from these samples belongs to the betasatellite species Tomato leaf curl Bangalore betasatellite. ToLCBV-C[IN:Hess:08] induced severe symptoms in Nicotiana benthamiana and Solanum lycopersicum plants when co-inoculated with the cognate betasatellite, Tomato leaf curl Bangalore betasatellite–[India:Hessarghatta:2008], ToLCBB-[IN:Hess:08] and with two other non-cognate betasatellites, Cotton leaf curl Multan betasatellite–[India:SriGanganagar:2002] and Luffa leaf distortion betasatellite–[India:Luffa:2004].

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

References

  1. Briddon RW, Bull SE, Mansoor S, Amin I, Markham PG (2002) Universal primers for the PCR-mediated amplification of DNA β: a molecule associated with some monopartite begomoviruses. Mol Biol Technol 20:315–318

    CAS  Google Scholar 

  2. Briddon RW, Brown JK, Moriones E, Stanley J, Zerbini M, Zhou X, Fauquet CM (2008) Recommendations for the classification and nomenclature of the DNA-β satellites of begomoviruses. Arch Virol 153:763–781

    Article  CAS  PubMed  Google Scholar 

  3. Chakraborty S (2008) Tomato leaf curl viruses from India. In: Mahy BWJ, Van Regenmortel MHV (eds) Encyclopedia of virology, vol 5. Elsevier Ltd, Amsterdam, pp 124–133

  4. Eini O, Akbar Benjatnia SA, Dogra S, Dry IB, Randles JW, Ali Rezaian M (2009) Identification of sequence elements regulating promoter activity and replication of a monopartite begomovirus associated DNA β satellite. J Gen Virol 90:253–260

    Article  CAS  PubMed  Google Scholar 

  5. Fauquet CM, Briddon RW, Brown JK, Moriones E, Stanley J, Zerbini M, Zhou X (2008) Geminivirus strain demarcation and nomenclature. Arch Virol 153:783–821

    Article  CAS  PubMed  Google Scholar 

  6. Haible D, Kober S, Jeske H (2006) Rolling circle amplification revolutionizes diagnosis and genomics of geminiviruses. J Virol Methods 135:9–16

    Article  CAS  PubMed  Google Scholar 

  7. Kirthi N, Maiya SP, Murthy MR, Savithri HS (2002) Evidence for recombination among the tomato leaf curl virus strains/species from Bangalore, India. Arch Virol 147:255–272

    Article  CAS  PubMed  Google Scholar 

  8. Muniyappa V, Venkatesh HM, Ramappa HK, Kulkarni RS, Zeiden M, Tarba C-Y, Ghanim M, Czosnek H (2000) Tomato leaf curl virus from Bangalore (ToLCV-Ban4): sequence comparison with Indian ToLCV isolates, detection in plants and insects, and vector relationships. Arch Virol 145:1583–1598

    Article  CAS  PubMed  Google Scholar 

  9. Nawaz-ul-Rehman MS, Mansoor S, Briddon RW, Fauquet CM (2009) Maintenance of an old world betasatellite by a new world helper begomovirus and possible rapid adaptation of the betasatellite. J Virol 83:9347–9355

    Article  CAS  PubMed  Google Scholar 

  10. Rouhibakhsh A, Priya J, Periasamy M, Haq QMI, Malathi VG (2008) An improved DNA isolation method and PCR protocol for efficient detection of multicomponents of begomovirus in legumes. J Virol Methods 147:37–42

    Article  CAS  PubMed  Google Scholar 

  11. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning a laboratory manual. 2nd edn. Vol I, Cold Spring Harbour Laboratory Press, New York

  12. Saunders K, Briddon RW, Stanley J (2008) Replication promiscuity of DNA-β satellites associated with monopartite begomoviruses; deletion mutagenesis of the Ageratum yellow vein virus DNA-β satellite localizes sequences involved in replication. J Gen Virol 89:3165–3172

    Article  CAS  PubMed  Google Scholar 

  13. Vasudeva RS, Sam Raj J (1948) A leaf curl disease of tomato. Phytopathology 38:364–369

    Google Scholar 

Download references

Acknowledgments

The financial support given by Department of Biotechnology Government of India [BT/PR/5351/AGR/16/482/2004-II, August 9, 2005] is gratefully acknowledged. We thank the scientists and staff at National Phytotron Facility, Indian Agricultural Research Institute, New Delhi -110012, for their guidance and support in growing plants under controlled conditions.

Author information

Authors and Affiliations

  1. Advanced Centre for Plant Virology, Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi, 110012, India

    Neha Tiwari, K. V. Padmalatha, V. B. Singh, Q. M. I. Haq & V. G. Malathi

Authors
  1. Neha Tiwari
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. K. V. Padmalatha
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. V. B. Singh
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Q. M. I. Haq
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. V. G. Malathi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to V. G. Malathi.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tiwari, N., Padmalatha, K.V., Singh, V.B. et al. Tomato leaf curl Bangalore virus (ToLCBV): infectivity and enhanced pathogenicity with diverse betasatellites. Arch Virol 155, 1343–1347 (2010). https://doi.org/10.1007/s00705-010-0710-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-010-0710-4

Keywords

Search

Navigation