Skip to main content
SpringerLink
Log in

Molecular diversity of the DNA-β satellites associated with tomato leaf curl disease in India

  • Brief Report
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

DNA-β satellites, referred to here as betasatellites, were found associated with tomato leaf curl disease (ToLCD) in India. The size of eight betasatellites isolated from different geographical locations in India varied from 1353 to 1424 nt; these molecules had an ORF βC1, an adenine-rich region, and a satellite conserved region. Their nucleotide sequence identity varied from 45 to 93%. In phylogenetic analysis, these betasatellites grouped according to their geographic locations rather than the host species. Two new betasatellites, tomato leaf curl Bangalore betasatellite and tomato leaf curl Maharashtra betasatellite, were identified.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

References

  1. Briddon RW, Brown JK, Moriones E, Stanley J, Zerbini M, Zhou X, Fauquet CM (2008) Recommendations for the classification and nomenclature of the DNA-beta satellites of begomoviruses. Arch Virol 153:763–781

    Article  CAS  PubMed  Google Scholar 

  2. Briddon RW, Bull SE, Amin I, Idris AM, Mansoor S, Bedford ID, Dhawan P, Rishi N, Siwatch SS, Abdel-Salam AM, Brown JK, Zafar Y, Markham PG (2003) Diversity of DNA β, a satellite molecule associated with some monopartite begomoviruses. Virology 312:106–121

    Article  CAS  PubMed  Google Scholar 

  3. Bull SE, Tsai WS, Briddon RW, Markham PG, Stanley J, Green SK (2004) Diversity of begomovirus DNA β satellites of non-malvaceous plants in east and south east Asia. Arch Virol 149:1193–1200

    Article  CAS  PubMed  Google Scholar 

  4. Dellaporta SL, Wood J, Hicks HB (1983) A plant DNA minipreparation: version II. Plant Mol Biol Rep 14:19–21

    Article  Google Scholar 

  5. Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98

    CAS  Google Scholar 

  6. Khan AJ, Idris AM, Al-Saady NA, Al-Mahruki MS, Al-Subhi AM, Brown JK (2008) A divergent isolate of Tomato yellow leaf curl virus from Oman with an associated DNAβ satellite: an evolutionary link between Asian and the Middle Eastern virus-satellite complexes. Virus Genes 36:169–176

    Article  CAS  PubMed  Google Scholar 

  7. Mandal B, Malathi VG, Varma A (1997) Systemic infection of Vigna mungo using the cloned DNAs of the blackgram isolate of mungbean yellow mosaic geminivirus through agroinoculation and transmission of the progeny virus by whiteflies. J Phytopathol 145:505–510

    Article  Google Scholar 

  8. Page RDM (1996) TREEVIEW: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12:357–358

    CAS  PubMed  Google Scholar 

  9. Rouhibakhsh A, Malathi VG (2005) A severe leaf crinkle disease of cowpea caused by Mungbean yellow mosaic India virus and a satellite DNA β. Plant Pathol 54:259

    Article  Google Scholar 

  10. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, vol I, 2nd edn. Cold Spring Harbour Laboratory Press, Cold Spring Harbour

    Google Scholar 

  11. Sivalingam PN, Malathi VG, Varma A (2004) Association of DNA β with mono- and bi-partite begomoviruses affecting tomato in India. In: 4th international geminivirus conference, Feb 12–14, 2004, Cape town, South Africa 16/2. W4-1

  12. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The Clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882

    Article  CAS  PubMed  Google Scholar 

  13. Ueda S, Onuki M, Hanada K, Takanami Y (2008) Unique grouping of the Far East Asian begomovirus complex based on sequence analyses of the DNA-A genome and associated DNA beta satellite molecules isolated from tomato, honeysuckle and Eupatorium plants in Japan. Arch Virol 153:417–426

    Article  CAS  PubMed  Google Scholar 

  14. Varma A, Malathi VG (2003) Emerging geminivirus problems: a serious threat to crop production. Ann Appl Biol 142:145–164

    Article  CAS  Google Scholar 

Download references

Author information

Author notes

  1. P. N. Sivalingam

    Present address: Central Institute for Arid Horticulture, ICAR, Bikaner, Rajasthan, 334006, India

Authors and Affiliations

  1. Division of Plant Pathology, Advanced Centre for Plant Virology, Indian Agricultural Research Institute, New Delhi, 110012, India

    P. N. Sivalingam, V. G. Malathi & A. Varma

Authors
  1. P. N. Sivalingam
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. V. G. Malathi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. Varma
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. Varma.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sivalingam, P.N., Malathi, V.G. & Varma, A. Molecular diversity of the DNA-β satellites associated with tomato leaf curl disease in India. Arch Virol 155, 757–764 (2010). https://doi.org/10.1007/s00705-010-0634-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-010-0634-z

Keywords

Search

Navigation