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Molecular characterization of Citrus tristeza virus isolates from the Northeastern Himalayan region of India

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Abstract

Citrus tristeza virus (CTV) isolates from the Darjeeling hills of the Northeastern Himalayan region of India were characterized by biological indexing, multiple molecular marker (MMM) analysis, heteroduplex mobility assay (HMA) and sequence analysis. Variability was studied using the CP gene and a 5′ ORF1a fragment of the CTV genome. HMA and sequence analysis of the 5′ ORF1a fragment classified Darjeeling isolates into two groups, whereas CP gene analysis provided evidence for three different groups. Darjeeling CTV isolates shared nucleotide sequence identities of 89–97 and 91–92% in the 5′ ORF1a fragment and CP gene, respectively, suggesting extensive diversity among CTV isolates from this Indian region.

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Acknowledgments

The author is grateful to Richard Lee and K.L. Manjunath, USDA-ARS, NCGRCD, Riverside, CA90507 for providing primers and amplified products of CTV isolates T2K and T38K, R. K. Jain, Head, Division of Plant Pathology, Indian Agricultural Research Institute (IARI), New Delhi, for laboratory facilities, and the Director, IARI, New Delhi, for financial support.

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  1. Plant Virology Unit, Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi, 110012, India

    K. K. Biswas

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  1. K. K. Biswas
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Correspondence to K. K. Biswas.

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Biswas, K.K. Molecular characterization of Citrus tristeza virus isolates from the Northeastern Himalayan region of India. Arch Virol 155, 959–963 (2010). https://doi.org/10.1007/s00705-010-0602-7

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