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Cold co-extraction of hemagglutinin and matrix M1 protein from influenza virus A by a combination of non-ionic detergents allows for visualization of the raft-like nature of the virus envelope

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Abstract

Membrane solubilization with a mixture of cold non-ionic detergents has been applied to isolate detergent-resistant membranes from intact virus A lipid bilayer. Association of the viral envelope glycoproteins and M1 into a raft lipid-protein complex was verified via detergent insolubility experiments, and the M1:HA stoichiometry of the proposed supramolecular complex was estimated via amino acid analysis. Electron microscopy and dynamic light scattering data revealed that these lipid-protein rafts form unilamellar vesicles with HA spikes on their surfaces similar to influenza virus virions. Together, our data suggest that the cold co-extraction technique visualizes the raft-like nature of the viral envelope and demonstrates the interaction of matrix M1 protein with the envelope.

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Acknowledgments

We thank Dr. L.V. Kordyukova for providing the bromelain-treated virus particles. This work was supported by the International Science and Technology Center (ISTC#2816/BTEP#82) and Russian Foundation for Basic Reseach (06-04-48728).

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Authors and Affiliations

  1. Department of Chromatography, A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Leninskie Gory, Moscow, 119992, Russia

    V. Radyukhin, N. Fedorova, A. Ksenofontov & L. Baratova

  2. Institute of Physico-Chemical Medicine, Russian Academy of Medical Sciences, Moscow, Russia

    M. Serebryakova

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  1. V. Radyukhin
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  2. N. Fedorova
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  3. A. Ksenofontov
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  4. M. Serebryakova
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  5. L. Baratova
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Correspondence to V. Radyukhin.

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Radyukhin, V., Fedorova, N., Ksenofontov, A. et al. Cold co-extraction of hemagglutinin and matrix M1 protein from influenza virus A by a combination of non-ionic detergents allows for visualization of the raft-like nature of the virus envelope. Arch Virol 153, 1977–1980 (2008). https://doi.org/10.1007/s00705-008-0214-7

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  • DOI: https://doi.org/10.1007/s00705-008-0214-7

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