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High frequency of BF mosaic genomes among HIV-1-infected children from Sao Paulo, Brazil

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Abstract

HIV-1 genetic diversity information from a pediatric population is scarce. This study enrolled 128 children living with HIV/AIDS, 103 antiretroviral-treated and 25 naive, from the Sao Paulo metropolitan area. Gag, pol and env regions were amplified, and drug resistance mutations, V3 loop, tropism and viral clades were evaluated. Drug resistance mutations among naïve children infected by vertical transmission were uncommon (4.2%), whereas most ARV-experienced children showed extensive mutation patterns. Clade B predominated at the pol region, but the analysis of the three regions concatenated showed 28% with BF mosaic structures. The most common V3 motif was GPGR, followed by GWGR in clade B samples and GPGQ in clade F samples. A predicted X4 phenotype was observed in 27%, without correlation to HIV clade. These findings expand the limited information on molecular characteristics of HIV-1 among children living with HIV/AIDS in the area and may provide information useful for monitoring the epidemic.

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Acknowledgments

This work was partially supported by Sao Paulo State Foundation for Research Support (FAPESP) 00/09668-4, FAPESP 05/52017-8, Ministry of Health-Aids Program AS No. 4.159-2006 and CNPq No. 307730/2006-9.

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Authors and Affiliations

  1. Laboratório de Retrovírus, Serviço de Virologia, Instituto Adolfo Lutz, Av. Dr. Arnaldo, 355, Sao Paulo, SP, 01246-902, Brazil

    Cristina Mendes de Oliveira, Rosangela Rodrigues & Luis Fernando de Macedo Brígido

  2. Santa Casa de Misericórdia de São Paulo, Sao Paulo, SP, Brazil

    Flávia Jacqueline Almeida & Eitan Naaman Berezin

  3. Universidade Estadual de Maringá, Maringá, PR, Brazil

    Marcia Crozatti

  4. Instituto de Infectologia Emilio Ribas, Sao Paulo, SP, Brazil

    Carla Maria Pasquareli Vazquez, Maria do Socorro Carneiro Ferrão, Alexandre Ely Campeas & Silvia Regina Marques

Authors
  1. Cristina Mendes de Oliveira
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  2. Flávia Jacqueline Almeida
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  3. Rosangela Rodrigues
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  4. Marcia Crozatti
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  5. Carla Maria Pasquareli Vazquez
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  6. Maria do Socorro Carneiro Ferrão
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  7. Alexandre Ely Campeas
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  8. Silvia Regina Marques
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  9. Eitan Naaman Berezin
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  10. Luis Fernando de Macedo Brígido
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Corresponding author

Correspondence to Luis Fernando de Macedo Brígido.

Additional information

Nucleotide sequence data reported are available in the GenBank database under the accession numbers EU189735, EU189737, EU189739–EU189743, EU189746–EU189748 and EU257861–EU258199.

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de Oliveira, C.M., Almeida, F.J., Rodrigues, R. et al. High frequency of BF mosaic genomes among HIV-1-infected children from Sao Paulo, Brazil. Arch Virol 153, 1799–1806 (2008). https://doi.org/10.1007/s00705-008-0178-7

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  • DOI: https://doi.org/10.1007/s00705-008-0178-7

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