Skip to main content
SpringerLink
Log in

Virulence domain of the RYMV genome-linked viral protein VPg towards rice rymv1–2-mediated resistance

  • Brief Report
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

Virulent variants of Rice yellow mottle virus (genus Sobemovirus) can emerge on the highly resistant rice cultivars Gigante and Bekarosaka. Non-synonymous mutations responsible for the breakdown of the recessive resistance gene rymv1–2 were located in the VPg after determination of its termini in the polyprotein P2a. The secondary structure of this protein was predicted to include a central α-helix. The two major amino acids related to virulence are located on the same side of this helix. The 3D topology and the biochemical properties of virulence mutations both suggested a direct site-to-site interaction between RYMV VPg and rice eIF(iso)4G encoded by rymv1.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

References

  1. Albar L, Bangratz-Reyser M, Hebrard E, Ndjiondjop M-N, Jones M, Ghesquiere A (2006) Mutations in the eIF(iso) 4G translation initiation factor confer high resistance of rice to Rice yellow mottle virus. Plant J 47:417–426

    Article  PubMed  CAS  Google Scholar 

  2. Ayme V, Souche S, Caranta C, Jacquemond M, Chadoeuf J, Palloix A, Moury B (2006) Different mutations in the genome-linked protein VPg of potato virus Y confer virulence on the pvr2(3) resistance in pepper. Mol Plant Microbe Interact 19:557–563

    Article  PubMed  CAS  Google Scholar 

  3. Brugidou C, Opalka N, Yeager M, Beachy RN, Fauquet C (2002) Stability of rice yellow mottle virus and cellular compartmentalization during the infection process in Oryza sativa (L.). Virology 297:98–108

    Article  PubMed  CAS  Google Scholar 

  4. Deleage G, Blanchet C, Geourjon C (1997) Protein structure prediction. Implications for the biologist. Biochimie 79:681–686

    Article  PubMed  CAS  Google Scholar 

  5. Glaser F, Steinberg DM, Vakser IA, Ben-Tal N (2001) Residue frequencies and pairing preferences at protein–protein interfaces. Proteins 43:89–102

    Article  PubMed  CAS  Google Scholar 

  6. Hébrard E, Pinel-Galzi A, Bersoult A, Siré C, Fargette D (2006) Emergence of a resistance-breaking isolate of Rice yellow mottle virus during serial inoculations is due to a single substitution in the genome-linked viral protein VPg. J Gen Virol 87:1369–1373

    Article  PubMed  CAS  Google Scholar 

  7. Hull R, Fargette D (2005) Sobemovirus. In: Fauquet C, Mayo MA, Maniloff J, Desselberger U, Ball LA (eds) Virus taxonomy. Eight report of the international committee on taxonomy of viruses. Academic Press, Elsevier, pp 885–890

    Google Scholar 

  8. Khan MA, Miyoshi H, Ray S, Natsuaki T, Suehiro N, Goss DJ (2006) Interaction of genome-linked protein (VPg) of Turnip mosaic virus (TuMV) with wheat germ translation initiation factors eIFiso4E and eIFiso4F. J Biol Chem 280:28002–28010

    Article  CAS  Google Scholar 

  9. Kouassi NK, N’Guessan P, Albar L, Fauquet C, Brugidou C (2005) Distribution and characterization of Rice yellow mottle virus: a threat to african farmers. Plant Dis 89:124–133

    Article  Google Scholar 

  10. Lopez-Moya JJ, Wang RY, Pirone TP (1999) Context of the coat protein DAG motif affects potyvirus transmissibility by aphids. J Gen Virol 80:3281–3288

    PubMed  CAS  Google Scholar 

  11. Makinen K, Tamm T, Naess V, Truve E, Puurand U, Munthe T, Saarma M (1995) Characterization of Cocksfoot mottle sobemovirus genomic RNA and sequence comparison with related viruses. J Gen Virol 76:2817–2825

    Article  PubMed  Google Scholar 

  12. Nicaise V, Gallois JL, Chafiai F, Allen LM, Schurdi-Levraud V, Browning KS, Candresse T, Caranta C, Le Gall O, German-Retana S (2007) Coordinated and selective recruitment of eIF4E and eIF4G factors for potyvirus infection in Arabidopsis thaliana. FEBS Lett 581:1041–1046

    Article  PubMed  CAS  Google Scholar 

  13. Pinel-Galzi A, Rakotomalala M, Sangu E, Sorho F, Kanyeka Z, Traoré O, Sérémé D, Poulicard N, Rabenantaondro Y, Séré Y, Konaté G, Ghesquière A, Hébrard E, Fargette D (2007) Theme and variations in the evolutionary pathways to virulence of an RNA plant virus species. PLoS Pathog 3:e180

    Article  PubMed  CAS  Google Scholar 

  14. Robaglia C, Caranta C (2006) Translation initiation factors: a weak link in plant RNA virus infection. Trends Plant Sci 11:40–45

    Article  PubMed  CAS  Google Scholar 

  15. Roudet-Tavert G, Michon T, Walter J, Delaunay T, Redondo E, Le Gall O (2007) Central domain of a potyvirus VPg is involved in the interaction with the host translation initiation factor eIF4E and the viral protein HcPro. J Gen Virol 88:1029–1033

    Article  PubMed  CAS  Google Scholar 

  16. Sadowy E, Milner M, Haenni AL (2001) Proteins attached to viral genomes are multifunctional. Adv Virus Res 57:185–262

    PubMed  CAS  Google Scholar 

  17. Satheshkumar PS, Lokesh GL, Savithri HS (2004) Polyprotein processing: cis and trans proteolytic activities of Sesbania mosaic virus serine protease. Virology 318:429–438

    Article  PubMed  CAS  Google Scholar 

  18. van der Wilk F, Verbeek M, Dullemans AM, van den Heuvel JF (1997) The genome-linked protein of Potato leafroll virus is located downstream of the putative protease domain of the ORF1 product. Virology 234:300–303

    Article  PubMed  Google Scholar 

  19. Yeam I, Cavatorta JR, Ripoll DR, Kang BC, Jahn MM (2007) Functional dissection of naturally occurring amino acid substitutions in eIF4E that confers recessive potyvirus resistance in plants. Plant Cell 19:2913–2928

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

We thank Olivier Legall and Benoit Moury for helpful discussions and Anne-Lise Haenni for constructive criticisms of the manuscript.

Author information

Authors and Affiliations

  1. UMR RPB 186, Institut de Recherche pour le Développement (IRD), BP 64501, 34394, Montpellier Cedex 5, France

    Eugénie Hébrard, Agnès Pinel-Galzi & Denis Fargette

Authors
  1. Eugénie Hébrard
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Agnès Pinel-Galzi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Denis Fargette
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Eugénie Hébrard.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hébrard, E., Pinel-Galzi, A. & Fargette, D. Virulence domain of the RYMV genome-linked viral protein VPg towards rice rymv1–2-mediated resistance. Arch Virol 153, 1161–1164 (2008). https://doi.org/10.1007/s00705-008-0087-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-008-0087-9

Keywords

Search

Navigation