Skip to main content

Advertisement

SpringerLink
Log in

Genetic stability of recombinant potato virus X virus vectors presenting foreign epitopes

  • Brief Report
  • Published:
Archives of Virology Aims and scope Submit manuscript

Summary

We investigated the genetic stability of recombinant potato virus X vectors presenting beet necrotic yellow vein virus (BNYVV) epitopes. Following N-terminal PVX coat protein (CP) fusion of the BNYVV epitopes, we inoculated Nicotiana benthamiana plants with recombinant (r)PVX and carried out five serial passages through systemically-infected plants. RT-PCR investigation of the BNYVV epitope sequences revealed the accumulation of several point mutations and deletions, predominantly affecting positively-charged residues. A comparison of the isoelectric point (pI) values and charges of the wild type and rCPs showed that the initial high rCP pI values had changed to values closer to that of the wild-type CP.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • LA Baratova NI Grebenshchikov AV Shishkov IA Kashirin JL Radavsky L Jarvekulg M Saarma (1992) ArticleTitleThe topography of the surface of potato virus X: tritium planigraphy and immunological analysis J Gen Virol 73 229–235 Occurrence Handle1371539 Occurrence Handle1:CAS:528:DyaK38Xht1Cmtb4%3D

    PubMed  CAS  Google Scholar 

  • D Baulcombe S Chapman S Santa Cruz (1995) ArticleTitleJellyfish green fluorescent protein as a reporter for virus infections Plant J 7 1045–1053 Occurrence Handle7599646 Occurrence Handle10.1046/j.1365-313X.1995.07061045.x Occurrence Handle1:CAS:528:DyaK2MXnsF2ltL4%3D

    Article  PubMed  CAS  Google Scholar 

  • M Bendahmane M Koo E Karrer RN Beachy (1999) ArticleTitleDisplay of epitopes on the surface of tobacco mosaic virus: impact of charge and isoelectric point of the epitope on virus-host interactions J Mole Biol 290 9–20 Occurrence Handle10.1006/jmbi.1999.2860 Occurrence Handle1:CAS:528:DyaK1MXktV2ktLg%3D

    Article  CAS  Google Scholar 

  • S Chapman G Hills J Watts D Baulcombe (1992) ArticleTitleMutational analysis of the coat protein gene of potato virus X: effects on virion morphology and viral pathogenicity Virology 191 223–230 Occurrence Handle1413503 Occurrence Handle10.1016/0042-6822(92)90183-P Occurrence Handle1:CAS:528:DyaK3sXjsFWm

    Article  PubMed  CAS  Google Scholar 

  • U Commandeur R Koenig R Manteuffel L Torrance P Luddecke R Frank (1994) ArticleTitleLocation, size, and complexity of epitopes on the coat protein of beet necrotic yellow vein virus studied by means of synthetic overlapping peptides Virology 198 282–287 Occurrence Handle7505070 Occurrence Handle10.1006/viro.1994.1031 Occurrence Handle1:CAS:528:DyaK2cXntFyksA%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • SS Cruz S Chapman AG Roberts IM Roberts DA Prior KJ Oparka (1996) ArticleTitleAssembly and movement of a plant virus carrying a green fluorescent protein overcoat Proc Natl Acad Sci USA 93 6286–6290 Occurrence Handle8692807 Occurrence Handle10.1073/pnas.93.13.6286 Occurrence Handle1:STN:280:BymB2s%2FltlQ%3D

    Article  PubMed  CAS  Google Scholar 

  • SS Cruz AG Roberts DA Prior S Chapman KJ Oparka (1998) ArticleTitleCell-to-cell and phloem-mediated transport of potato virus X. The role of virions Plant Cell 10 495–510 Occurrence Handle9548978 Occurrence Handle10.1105/tpc.10.4.495 Occurrence Handle1:CAS:528:DyaK1cXivFClsL0%3D

    Article  PubMed  CAS  Google Scholar 

  • ON Fedorkin A Merits J Lucchesi AG Solovyev M Saarma SY Morozov K Makinen (2000) ArticleTitleComplementation of the movement-deficient mutations in potato virus X: potyvirus coat protein mediates cell-to-cell trafficking of C-terminal truncation but not deletion mutant of potexvirus coat protein Virology 270 31–42 Occurrence Handle10772977 Occurrence Handle10.1006/viro.2000.0246 Occurrence Handle1:CAS:528:DC%2BD3cXisFers70%3D

    Article  PubMed  CAS  Google Scholar 

  • S Hendy ZC Chen H Barker S Santa Cruz S Chapman L Torrance W Cockburn GC Whitelam (1999) ArticleTitleRapid production of single-chain Fv fragments in plants using a potato virus X episomal vector J Immunol Methods 231 137–146 Occurrence Handle10648933 Occurrence Handle10.1016/S0022-1759(99)00150-7 Occurrence Handle1:CAS:528:DC%2BD3cXmtVKrsw%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • B Kimalov A Gal-On R Stav E Belausov T Arazi (2004) ArticleTitleMaintenance of coat protein N-terminal net charge and not primary sequence is essential for zucchini yellow mosaic virus systemic infectivity J Gen Virol 85 3421–3430 Occurrence Handle15483260 Occurrence Handle10.1099/vir.0.80417-0 Occurrence Handle1:CAS:528:DC%2BD2cXpsVSlsro%3D

    Article  PubMed  CAS  Google Scholar 

  • R Koenig JH Tremaine JF Shepard (1978) ArticleTitle In situ degradation of the protein chain of Potato virus X at the N- and C-termini J Gen Virol 38 329–337 Occurrence Handle1:CAS:528:DyaE1cXhsVOmu78%3D

    CAS  Google Scholar 

  • R Koenig L Torrance (1986) ArticleTitleAntigenic analysis of potato virus X by means of monoclonal antibodies J Gen Virol 67 2145–2151 Occurrence Handle1:CAS:528:DyaL28XlvVyqu7c%3D

    CAS  Google Scholar 

  • R Koenig DE Lesemann (1989) Potato virus X, potexvirus group AF Murant BD Harrison (Eds) AAB descriptions of plant viruses No 354 Association of Applied Biologists Warwick 1–5

    Google Scholar 

  • R Koenig P Lüddecke AM Haeberle (1995) ArticleTitleDetection of beet necrotic yellow vein virus strains, variants and mixed infections by examining single-strand conformation polymorphisms of immunocapture RT-PCR products J Gen Virol 76 2051–2055 Occurrence Handle7636486 Occurrence Handle1:CAS:528:DyaK2MXnsVCmtrc%3D Occurrence Handle10.1099/0022-1317-76-8-2051

    Article  PubMed  CAS  Google Scholar 

  • C Marusic P Rizza L Lattanzi C Mancini M Spada F Belardelli E Benvenuto I Capone (2001) ArticleTitleChimeric plant virus particles as immunogens for inducing murine and human immune responses against human immunodeficiency virus type 1 J Virol 75 8434–8439 Occurrence Handle11507188 Occurrence Handle10.1128/JVI.75.18.8434-8439.2001 Occurrence Handle1:CAS:528:DC%2BD3MXmsVarurk%3D

    Article  PubMed  CAS  Google Scholar 

  • C Porta VE Spall KC Findlay RC Gergerich CE Farrance GP Lomonossoff (2003) ArticleTitleCowpea mosaic virus-based chimaeras. Effects of inserted peptides on the phenotype, host range, and transmissibility of the modified viruses Virology 310 50–63 Occurrence Handle12788630 Occurrence Handle10.1016/S0042-6822(03)00140-5 Occurrence Handle1:CAS:528:DC%2BD3sXkt1yjt70%3D

    Article  PubMed  CAS  Google Scholar 

  • A Tamai T Meshi (2001) ArticleTitleCell-to-cell movement of potato virus X: the role of p12 and p8 encoded by the second and third open reading frames of the triple gene block Mol Plant Microbe Interact 14 1158–1167 Occurrence Handle11605955 Occurrence Handle1:CAS:528:DC%2BD3MXnsVeltLo%3D

    PubMed  CAS  Google Scholar 

  • K Uhde R Fischer U Commandeur (2005) ArticleTitleExpression of multiple foreign epitopes presented as synthetic antigens on the surface of potato virus X particles Arch Virol 150 327–340 Occurrence Handle15503224 Occurrence Handle10.1007/s00705-004-0402-z Occurrence Handle1:CAS:528:DC%2BD2MXksVKhsQ%3D%3D

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute for Molecular Biotechnology, RWTH Aachen University, Aachen, Germany

    K. Uhde-Holzem, R. Fischer & U. Commandeur

  2. Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Aachen

    R. Fischer

Authors
  1. K. Uhde-Holzem
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. Fischer
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. U. Commandeur
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Uhde-Holzem, K., Fischer, R. & Commandeur, U. Genetic stability of recombinant potato virus X virus vectors presenting foreign epitopes. Arch Virol 152, 805–811 (2007). https://doi.org/10.1007/s00705-006-0892-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-006-0892-y

Keywords

Search

Navigation