Skip to main content
SpringerLink
Log in

Detailed cytomorphological investigations through male meiosis of polypetalous plants from the Kashmir Himalaya

  • Original Article
  • Published:
Plant Systematics and Evolution Aims and scope Submit manuscript

Abstract

Cytomorphological studies were made on 322 populations of 150 species/153 taxa belonging to 79 genera and 23 families of flowering plants. Out of these, 14 species were cytologically worked out for the first time on a worldwide basis along with B-chromosomes (0–1B) recorded for the first time in Agrimonia eupatoria. Similarly, 29 species were reported with varied chromosomal reports on a worldwide basis. The chromosome numbers ranged from 2n = 10 to 2n = 96 and posed high amount of intraspecific genetic diversity when seen in relation to other reports for the worked out species in light of previous chromosomal databases on India and world basis. The different levels of ploidy in these species/taxa vary from 2x to 12x with the overall polyploidy being 27.45 % and predominant in members belonging to the genera Ranunculus and Epilobium as well as families Ranunculaceae and Rosaceae. In the taxa showing anomalous meiotic behavior, cytomixis is found to be quite common and associated with various meiotic abnormalities in the meiocytes, which ultimately leads to pollen sterility and formation of variable sized pollen grains.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Baptista-Giacomelli FR, Palgliarini MS, Almeida JL (2000) Meiotic behavior in several Brazilian oat cultivars (Avena sativa L.). Cytologia 65:371–378

    Article  Google Scholar 

  • Basavaiah D, Murthy TCS (1987) Cytomixis in pollen mother cells of Urochloa panicoides P. Beauv. (Poaceae). Cytologia 52:69–74

    Article  Google Scholar 

  • Bertagnolle F, Thompson JD (1995) Gametes with the somatic chromosome number mechanisms of their formation and role in the evolution of autopolyploid plants. New Phytol 129:1–22

    Article  Google Scholar 

  • Bhattacharjee SK (1953) Cytogenetics of Lens esculenta Monesch. Caryologia 5:159–166

    Article  Google Scholar 

  • Chauhan AKS (1981) Cytomixis in Papaver rhoeas. In: Manna GK, Sinha U (eds) Perspectives in cytology and genetics. Hindasia Publishers, New Delhi, pp 309–312

  • Cheng KC, Nieh HW, Yang CL (1975) Light and electron microscopical observations on cytomixis and the study of its relation to variation and evolution. Acta Bot Sin 17:60–69

    Google Scholar 

  • Cifuentes M, Grandont L, Moore G, Chévre AM, Jenczewsk E (2010) Genetic regulation of meiosis in polyploid species: new insights into an old question. New Phytol 186:29–36

    Article  PubMed  CAS  Google Scholar 

  • Cires E, Cuesta C, Peredo EL, Revilla MA, Fernández Prieto JA (2009) Genome size variation and morphological differentiation within Ranunculus parnassifolius group (Ranunculaceae) from calcareous screes in the Northwest of Spain. Pl Syst Evol 281:193–208

    Article  Google Scholar 

  • Consolaro MEL, Pagliarini MS (1996) Spontaneous chromosome stickiness in microsporocytes of Centella asiatica (L.) Urban (Umbelliferae). Cytologia 61:57–61

    Article  Google Scholar 

  • Dar GH, Koul ST, Naqshi AR, Khuroo AA, Malik AH (2011) A new species of Corydalis DC. (Fumariaceae) from Kashmir North-west Himalaya India. Taiwania 56:305–308

    Google Scholar 

  • de Wet JMJ (1980) Origin of polyploids. In: Lewis WH (ed) Polyploidy: biological relevance. Plenum Press, New York, pp 3–16

    Google Scholar 

  • Dhar U, Kachroo P (1983) Alpine flora of Kashmir Himalayas. Scientific Publishers, Jodhpur

    Google Scholar 

  • El-Morsy Sh I, Dorra MDM, El-Hady, Abd EAA, Hiaba AAA, Mohamed AY (2009) Comparative studies on diploid and tetraploid levels of Nicotiana alata. Acad J Pl Sci 2:182–188

    Google Scholar 

  • Fadaie F, Sheidai M, Asadi M (2010) Cytological study the genus Arenaria L. (Caryophyllaceae). Caryologia 63:149–156

    Article  Google Scholar 

  • Fernandes A, Queiro’s M (1978) Contribution a la connaissance cytotaxinomique des Spermatophyta du Portugal. IV.Leguminosae. Bol Soc Broter 52(Suppl. 3):79–164

    Google Scholar 

  • Gaeta RT, Pires JC (2010) Homeologous recombination in allopolyploids: the polyploid ratchet. New Phytol 186:18–28

    Article  PubMed  CAS  Google Scholar 

  • Gohil RN, Koul AK (1978) Structural hybridity in Allium consanguineum. Cytologia 43:243–247

    Article  Google Scholar 

  • Grant V (1963) The origin of adaptations. Columbia University Press, New York

    Google Scholar 

  • Guan JZ, Wang JJ, Cheng ZH, Liu Y, Li ZY (2012) Cytomixis and meiotic abnormalities during microsporogenesis are responsible for male sterility and chromosome variations in Houttuynia cordata. Genet Mol Res 11:121–130

    Article  PubMed  Google Scholar 

  • Habib AF, Chennaveeraiah MS (1976) Cytomixis in Capsicum annuum L. J Cytol Genet 11:51–55

    Google Scholar 

  • Haq Eu, Dar GH, Wafai BA, Khuroo AA (2011) Carex brownii Tuckermen (Cyperaceae)—a new record for India. Pleione 5:325–327

    Google Scholar 

  • Haroun SA, Al Shehri AM, Al Wadie HM (2004) Cytomixis in the microsporogenesis of Vicia faba L. (Fabaceae). Cytologia 69:7–11

    Article  Google Scholar 

  • Iqbal M, Siddique MAA (2004) Prospects of establishing plant based pharamaticual units in Kashmir. Plant resource management for entrepreneurship development, Kashmir, pp 1–6

    Google Scholar 

  • Jeelani SM, Rani S, Kumar S, Kumari S, Gupta RC (2010) Cytomorphological diversity in species of Impatiens Linn. (Balsaminaceae) from Western Himalayas (India). Cytologia 75:379–387

    Article  Google Scholar 

  • Jeelani SM, Kumari S, Gupta RC (2011a) New chromosome number reports in some polypetalous species from Kashmir Himalayas. Chrom Sci 14:9–16

    Google Scholar 

  • Jeelani SM, Kumari S, Gupta RC (2011b) In IOPB chromosome number reports. Taxon 60:1788–1789

    Google Scholar 

  • Jeelani SM, Rani S, Kumar S, Kumari S, Gupta RC (2011c) Meiotic studies in some members of Caryophyllaceae Juss. from the Western Himalayas. Acta Biol Craco Ser Bot 53:86–95

    Google Scholar 

  • Jeelani SM, Rani S, Kumar S, Kumari S, Gupta RC (2011d) Evaluation of cytomorphological diversity in Filipendula vestita (Wall. ex G. Don.) Maxim. (Rosaceae) from Western Himalayas. Cytologia 76:403–410

    Google Scholar 

  • Jeelani SM, Kumari S, Gupta RC (2012a) Meiotic studies in some selected Angiosperms from the Kashmir Himalayas. J Syst Evol 50:244–257

    Article  Google Scholar 

  • Jeelani SM, Kumari S, Gupta RC (2012b) Male meiosis in Lotus corniculatus L. Pl Syst Evol 298(10):1977–1985

    Article  Google Scholar 

  • Jeelani SM, Rani S, Kumar S, Kumari S, Gupta RC (2013) Cytological studies of Brassicaceae Burn. (Cruciferae Juss.) from Western Himalayas. Cytol Genet 47(1):20–28

    Article  Google Scholar 

  • Jiang Y, Ding C, Yue H, Yang R (2011) Meiotic behavior and pollen fertility of five species in the genus Epimedium. Afr J Biotech 10:16189–16192

    Article  CAS  Google Scholar 

  • Kim JS, Oginuma K, Tobe H (2009) Syncyte formation in the microsporangium of Chrysanthemum (Asteraceae): a pathway to intraspecific polyploidy. J Plant Res 122:439–444

    Article  PubMed  Google Scholar 

  • Koul KK, Gohil RN (1991) Cytogenetic studies on some Kashmir grasses. VIII. Tribe Agrostideae, Festuceae and Paniceae. Cytologia 56:437–452

    Article  Google Scholar 

  • Kumar S (2012) Exploration and evaluation of cytomorphological diversity in Polypetalae from district Sirmaur (Himachal Pradesh). Ph.D. Thesis Punjabi University, Patiala

  • Kumar G, Tripathi R (2007) Anomalous nucleolar and chromosomal organization in induced phenodeviants of grass pea. Cytologia 79:345–350

    Article  Google Scholar 

  • Kumar S, Jeelani SM, Rani S, Kumari S, Gupta RC (2011a) Exploration of intraspecific cytomorphological diversity in Agrimonia eupatoria L. (Rosaceae) from Western Himalayas (India). Cytologia 76:81–88

    Article  Google Scholar 

  • Kumar S, Jeelani SM, Rani S, Gupta RC, Kumari S (2011b) Cytomorphological studies of genus Saxifraga L. from Western Himalayas. Nucleus 54:77–83

    Article  Google Scholar 

  • Kumar S, Jeelani SM, Rani S, Gupta RC, Kumari S (2013a) Cytology of five species of subfamily Papaveroideae from the Western Himalayas. Protoplasma 250(1):307–316

    Article  PubMed  Google Scholar 

  • Kumar S, Jeelani SM, Rani S, Gupta RC, Kumari S (2013b) Meiotic analysis in some species of Ranunculus L. (Ranunculaceae) from Western Himalayas. J Japanse Bot 88(1):46–53

    Google Scholar 

  • Kumar S, Jeelani SM, Rani S, Kumari S, Gupta RC (2013c) Cytological evaluation of Apiaceae Lindl. from Western Himalayas. Cytol Genet (accepted)

  • Lakshmi N, Prakash NS, Harini I, Rama YR (1989) A case of spontaneous cytomixis coupled with desynapsis in Capsicum annum L. Cytologia 54:287–291

    Article  Google Scholar 

  • Luttikhuizen PC, Stift M, Kuperus P, Van Tienderen PH (2007) Genetic diversity in diploid vs. tetraploid Rorippa amphibian (Brassicaceae). Mol Ecol 16:3544–3553

    Article  PubMed  CAS  Google Scholar 

  • Lydia G, Rao RKG (1982) Abnormal spindle behaviour in induced autotetraploid Physalis pubescens L. Theor Appl Genet 63:125–127

    Article  PubMed  CAS  Google Scholar 

  • Malallah GA, Attia TA (2003) Cytomixis and its possible evolutionary role in a Kuwait population of Diplotaxis harra (Boraginaceae). Bot J Linn Soc 143:169–175

    Article  Google Scholar 

  • Malik AH, Khuroo AA, Dar GH, Khan ZS (2011) Ethnomedicinal uses of some plants in the Kashmir Himalaya India. India J Trad Know 10:362–366

    Google Scholar 

  • Mandal A, Datta AK, Gupta S, Paul R, Saha A, Ghosh BK, Bhattacharya A, Iqbal M (2013) Cytomixis—a unique phenomenon in animal and plant. Protoplasma. doi:10.1007/s00709-013-0493-z

    PubMed  Google Scholar 

  • Mary TN, Suvarnalatha B (1981) Cytomixis and deviation of chromosome numbers in pollen mother cells of Gossypium species. J Indian Bot Soc 60:74

    Google Scholar 

  • Nakagawa M (2006) Ploidy geographical distribution and morphological differentiation of Parasenecio auriculata (Senecioneae Asteraceae) in Japan. J Plant Res 119:51–61

    Article  PubMed  Google Scholar 

  • Nirmala A, Rao PN (1996) Genesis of chromosomal numerical mosaicism in higher plants. Nucleus 39:151–175

    Google Scholar 

  • Omidbaigi R, Mirzaee M, Hassani ME, Sedghi Moghadam M (2010) Induction and identification of polyploidy in basil (Ocimum basilicum L.) medicinal plant by colchicine treatment. Int J Plant Prod 4:87–98

    Google Scholar 

  • Pagliarini MS, Pereira MAS (1992) Meiotic studies in Pilocarpus pennatifolius Lem. (Rutaceae). Cytologia 57:231–235

    Article  Google Scholar 

  • Panigrahi G, Dikshit BK (1987) Systematics of the genus Potentilla L. (Rosaceae Juss.)—its infrageneric classification and evolutionary trends. Bull Bot Surv India 27:177–196

    Google Scholar 

  • Rani S (2012) Exploration and evaluation of cytomorphological diversity in Polypetalae from district Kangra (Himachal Pradesh). Ph.D. Thesis Punjabi University, Patiala

  • Rani S, Kumar S, Jeelani SM, Gupta RC, Kumari S (2010a) New chromosome number reports in some polypetalous plants from Western Himalayas (India). Chrom Bot 5:49–53

    Article  Google Scholar 

  • Rani S, Kumar S, Jeelani SM, Gupta RC, Kumari S (2010b) Effect of cytomixis on male meiosis in populations of Clematis grata Wall. from Western Himalayas. Chrom Bot 5:61–64

    Article  Google Scholar 

  • Rani S, Kumar S, Jeelani SM, Kumari S, Gupta RC (2011) Meiotic studies in some members of Ranunculaceae from Western Himalaya (India). Caryologia 64:405–418

    Google Scholar 

  • Rani S, Kumar S, Jeelani SM, Kumari S, Gupta RC (2012) Additions to the cytologically investigated species of Potentilla L. (Rosaceae) from India. Pl Syst Evol 298:485–497

    Article  Google Scholar 

  • Rani S, Kumar S, Jeelani SM, Kumari S, Gupta RC (2013) Impaired male meiosis, morphology and distribution pattern of different cytotypes of Bupleurum lanceolatum Wall. (Apiaceae) from the Western Himalayas. Pl Syst Evol. doi:10.1007/s00606-013-0871-1

    Google Scholar 

  • Rao PN, Kanganadhaam P, Nirmala A (1990) Behaviour of a sticky desynaptic mutant in pearl millet. Genetica 81:221–227

    Article  Google Scholar 

  • Saggoo MIS, Farooq U (2011) Cytology of Rheum a vulnerable medicinal plant from Kashmir Himalaya. Chrom Bot 6:41–44

    Article  Google Scholar 

  • Shabir AP, Nawchoo IA, Wani AA (2012) Chromosomal stickiness and breeding behaviour in Inula racemosa—a critically endangered medicinal herb of N.W. Himalayas. In: Nat. Symp. Pl. Cytogenetics: new approaches, Punjabi University, Patiala, 23–24 Feb 2012

  • Sharma G, Gohil RN (2003) Cytology of Allium roylei Stearn. I. Meiosis in a population with complex interchanges. Cytologia 68:115–119

    Google Scholar 

  • Sheidai M, Attaei S (2005) Meiotic studies of some Stipa (Poaceae) species and population in Iran. Cytologia 70:23–31

    Article  Google Scholar 

  • Sheidai M, Bagheri-Shabestarei ES (2007) Cytotaxonomy of some Festuca species and populations in Iran. Acta Bot Croatica 66:143–151

    Google Scholar 

  • Sheidai M, Fadael F (2005) Cytogenetic studies in some Bromus L. species sec. Genea J Genetics 84:189–194

    Article  Google Scholar 

  • Sheidai M, Koobaz P, Zehzad B (2003) Meiotic studies of some Avena species and populations in Iran. J Sci I R Iran 14:121–131

    Google Scholar 

  • Sheidai M, Nouroozi M (2005) Cytological studies on some species of Bromus sect. Bromus. Botanica Lithuanica 11:141–150

    Google Scholar 

  • Sheidai M, Nikoo M, Gholipour A (2008) Cytogenetic variability and new chromosome number reports in Silene L. species (Sect. Lasiostemones Caryophyllaceae). Acta Biol Szeged 52:313–319

    Google Scholar 

  • Siddique MAA, Wafai BA, Dhar U (1990) Chromosome complement and nucleolar organization in Podophyllum hexandrum Royle. Genetica 82:59–62

    Article  Google Scholar 

  • Singh DK, Uniyal BP, Mathur R (1999) Jammu & Kashmir. In: Mudgal V, Hajra PK (eds) Floristic diversity and conservation strategies in India, II. Dehradun, pp 905–974

  • Singhal VK (1982) Cytomorphological studies on some members of Polypetalae from Northern and Central India. Ph.D. Thesis Department of Botany, Punjabi University, Patiala

  • Singhal VK, Gill BS (1985) Cytomixis in some woody species. Biologica 1:168–175

    Google Scholar 

  • Sinha SSN, Godward MBE (1972) Radiation studies in Lotus cultivars. Meiosis: abnormalities due to gamma radiation and its consequences. Cytologia 37:685–698

    Article  Google Scholar 

  • Soltis DE, Soltis PS, Schemske DW, Hancock JF, Thompson JN, Husband BC, Judd WS (2007) Autopolyploidy in Angiosperms: have we grossly underestimated the number of species? Taxon 56:13–30

    Google Scholar 

  • Španiel S, Marhold K, Hodálová I, Lihová J (2008) Diploid and tetraploid cytotypes of Centaurea stoebe (Asteraceae) in central Europe: morphological differentiation and cytotype distribution patterns. Folia Geobot 43:131–158

    Article  Google Scholar 

  • Stebbins GL (1938) Cytological characteristics associated with the different growth habits in the dicotyledons. Am J Bot 25:189–198

    Article  Google Scholar 

  • Stebbins GL (1950) Variation and evolution in plants. Columbia University Press, New York

    Google Scholar 

  • Stebbins GL (1958) Longevity habitat and release of genetic variability in the higher plants. Cold Spring Herb Symp Plant Biol 23:365–378

    Article  CAS  Google Scholar 

  • Tak MA, Wafai BA (1997) Chromosome enumeration and pollen mother cell meiosis of some wild and cultivated members of family Ranunculaceae. Oriental Sci 2:1–12

    Google Scholar 

  • Tarar JL, Dnyansagar VR (1980) Comparison of ethyl methane sulphonate and radiation induced meiotic abnormalities in Turnera ulmifolia L. var angustifolia wild. Cytologia 45:221–231

    Article  CAS  Google Scholar 

  • Thomas PT, Revell SH (1946) Secondary association and heterochromatic attraction. I. Cicer arietinum. Ann Bot 38:159–164

    Google Scholar 

  • Villeux R (1985) Diploid and polyploidy gametes in crop plants: mechanisms of formation and utilization in plant breeding. In: Janick J (ed) Plant breed rev, 3rd edn. AVI Publishing, Wesport, p 442

    Google Scholar 

  • Vorsa N, Bingham ET (1979) Cytology of 2n pollen formation in diploid alfa Medicago sativa. Can J Genet Cytol 21:525–530

    Google Scholar 

  • Wang SYu, Yui ChKh, Lee S, Wang ChI, Chzan GCh (2004) Ultrastructure and possible origin of the cytoplasmic channels providing connection between cells of anther vegetative tissues. Fiziol Rastenii 51:110–120

    Google Scholar 

  • Yen C, Yan JL, Sun GL (1993) Intermeiocyte connections and cytomixis in intergeneric hybrid of Roegneria ciliaris (Trin.) Nevski with Psathyrostachys huashanica Keng. Cytologia 58:187–193

    Article  Google Scholar 

Download references

Acknowledgments

The authors are grateful to the University Grants Commission, New Delhi under the DRS SAP II and ASIST programs. Additional support was provided by the UGC under the Dr. D.S. Kothari Post-Doctoral Fellowship Scheme [Award Letter No. F.4-2/2006 (BSR)/13-880/2013(BSR)] to Dr. Syed Mudassir Jeelani. Our thanks are also due to the Head, Department of Botany, Punjabi University, Patiala, for necessary laboratory facilities. We are also highly thankful to the Director Botanical Survey of India, Dehra Dun for their help in the identification of the plant species.

Author information

Authors and Affiliations

  1. Division of Floriculture, Medicinal and Aromatic Plants, SK University of Agriculture Sciences and Technology of Kashmir, Shalimar Srinagar, J&K, 191121, India

    Syed Mudassir Jeelani & Mohmmad Abubakar Ahmad Siddique

  2. Department of Botany, Punjabi University, Patiala, Punjab, 147002, India

    Santosh Kumari & Raghbir Chand Gupta

Authors
  1. Syed Mudassir Jeelani
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Santosh Kumari
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Raghbir Chand Gupta
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Mohmmad Abubakar Ahmad Siddique
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Syed Mudassir Jeelani.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jeelani, S.M., Kumari, S., Gupta, R.C. et al. Detailed cytomorphological investigations through male meiosis of polypetalous plants from the Kashmir Himalaya. Plant Syst Evol 300, 1175–1198 (2014). https://doi.org/10.1007/s00606-013-0954-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00606-013-0954-z

Keywords

Search

Navigation