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Analysis of hybridization between tetraploid Lotus corniculatus and diploid Lotus stepposus (Fabaceae-Loteae): morphological and molecular aspects

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Abstract

We analyzed variability of morphological characters and genetic polymorphism of inter-simple sequence repeat (ISSR) markers in nine natural populations of three Lotus species from Eastern Europe, aiming to provide insights into the nature of the species L. ucrainicus. Nuclear ribosomal internal transcribed spacer (nrITS) was used as an additional molecular marker for a small subset of accessions. Analysis of variance, and principal coordinate and principal component analyses were applied for morphological data study. Cluster analysis [unweighted pair-group method with arithmetic mean (UPGMA)], principal coordinate analysis, and analysis of population genetic structure were used for ISSR pattern study. Morphological and genetic (ISSR, nrITS) evidence suggested hybrid origin of L. × ucrainicus as a result of hybridization between tetraploid species L. corniculatus and diploid species L. stepposus. We conclude that L. × ucrainicus may represent a case of hybrid speciation in statu nascendi, occurring before our very eyes.

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References

  • Anderson EC, Thompson EA (2002) A model-based method for identifying species hybrids using multilocus genetic data. Genetics 160:1217–1229

    PubMed  CAS  Google Scholar 

  • Bleeker W, Hurka H (2001) Introgressive hybridization in Rorippa (Brassicaceae): gene flow and its consequences in natural and anthropogenic habitats. Mol Ecol 10:2013–2022

    Article  PubMed  CAS  Google Scholar 

  • Bornet B, Branchart M (2001) Nonanchored Inter Simple Sequence Repeat (ISSR) Markers: reproducible and specific tools for genome fingerprinting. Plant Mol Biol Rep 19:209–215

    Article  CAS  Google Scholar 

  • Bonin A, Bellemain E, Eidesen B, Pompanon F, Brochmann C, Taberlet P (2004) How to track and access genotyping errors in population genetic studies. Mol Ecol 13:3261–3273

    Article  PubMed  CAS  Google Scholar 

  • Buntjer JB (2000) Cross Checker: computer assisted scoring of genetic AFLP data. In: Plant & animal genome VIII conference. San Diego, CA, Jan 9–12

  • Degtjareva GV, Kramina TE, Sokoloff DD, Samigullin TH, Vallejo-Roman CM, Antonov AS (2006) Phylogeny of the genus Lotus (Leguminosae, Loteae): evidence from nrITS sequences and morphology. Can J Bot 84:813–830

    Article  Google Scholar 

  • Degtjareva GV, Kramina TE, Sokoloff DD, Samigullin TH, Sandral G, Vallejo-Roman CM (2008) New data on nrITS phylogeny of Lotus (Leguminosae, Loteae). Wulfenia 15:35–49

    Google Scholar 

  • Edgar RC (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 32:1792–1797

    Article  PubMed  CAS  Google Scholar 

  • Evanno G, Regnaut S, Goudet J (2005) Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Mol Ecol 14:2611–2620

    Article  PubMed  CAS  Google Scholar 

  • Falush D, Stephens M, Pritchard J (2007) Inference of population structure using multilocus genotype data: dominant markers and null alleles. Molec Ecol Notes 7:574–578

    Article  CAS  Google Scholar 

  • Gauthier P, Roselyne L, Bedecarrats A (1998) Genetic variation and gene flow in Alpine diploid and tetraploid population of Lotus (L. alpinus (D.C.) Schleicher/L. corniculatus L.). II. Insights from RFLP of chloroplast DNA. Heredity 80:694–701

    Article  CAS  Google Scholar 

  • Goldman JJ (2008) The use of ISSR markers to identify Texas bluegrass interspecific hybrids. Plant Breed 127:644–646. doi:10.1111/j.1439-0523.2008.01526.x

    Article  CAS  Google Scholar 

  • Gower JC (1971) A general coefficient of similarity and some of its properties. Biometrics 27:857–871

    Article  Google Scholar 

  • Grant WF, Small E (1996) The origin of the Lotus corniculatus (Fabaceae) complex: a synthesis of diverse evidence. Can J Bot 74:975–989

    Article  Google Scholar 

  • Grishanin AK, Shekhovtsov SV, Boykova TV, Akifyev AP, Zhimulev IF (2006) The problem of chromatin diminution at the border of the XX and XXI centuries. Tsitologiya 48(14):379–397 (in Russian)

    Google Scholar 

  • Hammer Ø, Harper DAT, Ryan PD (2001) PAST: Paleontological Statistics Software Package for Education and Data Analysis. Palaeontologia Electronica 4(1):1–9. http://palaeo-electronica.org/2001_1/past/issue1_01.htm

  • Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acids Symp 41:95–98

    CAS  Google Scholar 

  • Jaccard P (1912) The distribution of flora in the alpine zone. New Phytol 11(2):37–50

    Article  Google Scholar 

  • Kramina TE (1999) A contribution to the taxonomic revision of the Lotus corniculatus complex (Leguminosae, Loteae) in the European part of the former USSR. Syst Geogr Pl 68:265–279

    Article  Google Scholar 

  • Kramina TE (2000) A new species of Lotus L. (Leguminosae) from L. corniculatus group. Bull Mosc Soc Nat Biol Ser 105(1):35–40 (in Russian)

    Google Scholar 

  • Kramina TE, Schanzer IA (2010) Hybridization between two diploid species of Lotus (Fabaceae) in Volgograd region. Bot Zhurn 95:820–833 (in Russian)

    Google Scholar 

  • Minjaev NA, Ulle ZG (1987) Lotus L. In: Fedorov AA (ed) Flora Part Europ URSS. Nauka, Leningrad. 6:103–115 (in Russian)

  • Nagaoka T, Ogihara Y (1997) Applicability of inter-simple sequence repeat polymorphisms in wheat for use as DNA markers in comparison to RFLP and RAPD markers. Theor Appl Genet 94:597–902

    Article  CAS  Google Scholar 

  • Negri V, Veronesi F (1989) Evidence for the existence of 2n gametes in Lotus tenuis Wald. et Kit. (2n = 2x = 12): their relevance in evolution and breeding of Lotus corniculatus L. (2n = 4x = 24). Theor Appl Genet 78:400–404

    Article  Google Scholar 

  • Pritchard JK, Stephens M, Donnelly P (2000) Inference of population structure using multilocus genotype data. Genetics 155:945–959

    PubMed  CAS  Google Scholar 

  • Reddy MP, Sarla N, Siddiq EA (2002) Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding. Euphytica 128:9–17

    Article  Google Scholar 

  • Rieseberg LH, Wendel JF (1993) Introgression and its consequences in plants. In: Harrison RG (ed) Hybrid zones and the evolutionary process. Oxford University Press, Oxford, pp 70–109

    Google Scholar 

  • Reynaud J, Jay M, Blaise S (1991) Evolution and differentiation of populations of Lotus corniculatus s.l. (Fabaceae) from the southern French Alps (Massif du Ventoux and Montagne de Lure). Can J Bot 69:2286–2290

    Article  Google Scholar 

  • Rim YW, Beuselinck PR (1992) Verification of crossing between Lotus corniculatus (2n = 4x = 24) and 2n pollen producing L. tenuis (2n = 2x = 12). Agron Abstr:112

  • Rim YW, Beuselinck PR (1996) Cytology of 2N pollen formation and pollen morphology in diploid Lotus tenuis (Fabaceae). Am J Bot 83:1057–1062

    Article  Google Scholar 

  • Sandral G, Degtjareva GV, Kramina TE, Sokoloff DD, Samigullin TH, Hughes S, Valiejo-Roman CM (2009) Are Lotus creticus and Lotus cytisoides (Leguminosae) closely related species? Evidence from nuclear ribosomal ITS sequence data. Genet Resour Crop Evol. doi:10.1007/s10722-009-9486-3

  • Schönswetter P, Suda J, Popp M, Weiss-Schneeweiss H, Brochmann C (2007) Circumpolar phylogeography of Juncus biglumis (Juncaceae) inferred from AFLP fingerprints, cpDNA sequences, nuclear DNA content and chromosome numbers. Mol Phylogenet Evol 42:92–103

    Article  PubMed  Google Scholar 

  • StatSoft Inc (2006) STATISTICA (data analysis software system), version 7.1. http://www.statsoft.com

  • Valiejo-Roman CM, Terentieva EI, Samigullin TH, Pimenov MG (2002) Relationships among genera in Saniculoideae and selected Apioideae (Umbelliferae) inferred from nrITS sequences. Taxon 51:91–103

    Article  Google Scholar 

  • Wolfe AD, Xiang Q-Y, Kephart SR (1998) Assessing hybridization in natural populations of Penstemon (Scrophulariaceae) using hypervariable intersimple sequence repeat (ISSR) bands. Molec Ecol 7:1107–1125

    Article  CAS  Google Scholar 

  • Wolfe AD, Liston A (1998) Contributions of PCR-based methods to plant systematics and evolutionary biology. In: Soltis DE, Soltis PS, Doyle JJ (eds) Molecular systematics of plants II. Chapman Hall, New York, pp 43–86

    Chapter  Google Scholar 

  • Záveská E, Fér T, Šída O, Leong-Škorničová J, Sabu M, Marhold K (2011) Genetic diversity patterns in Curcuma reflect differences in genome size. Bot J Linn Soc 165:388–401

    Article  Google Scholar 

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Acknowledgments

This work was supported by grants from the Russian Fund of Fundamental Research (project 09-04-01323) and from the Ministry of Education and Science of Russia (FCP “Kadry,” NK-541P/P314). We express our gratitude to Dr. Jan Suda from Charles University (Prague) for performing flow cytometry analysis. We are very thankful to A.V. Troizky and I.A. Schanzer for consultation regarding application of methods of molecular data analyses. We wish to thank G. Yu. Klinkova and V.M.O. Stapko for their help in fieldwork and plant sampling, and D.D. Sokoloff and O.V. Yurtseva for helpful discussion of the results obtained.

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Authors and Affiliations

  1. Department of Higher Plants, Biological Faculty, Lomonosov Moscow State University, Leniskie Gory, 1, Building 12, Moscow, 119991, Russia

    T. E. Kramina

  2. Botanical Garden, Biological Faculty, Lomonosov Moscow State University, Leniskie Gory, Moscow, 119991, Russia

    G. V. Degtjareva

  3. A.N. Severtsov Institute of Ecology and Evolution, Leninskij prosp., 33, Moscow, 119071, Russia

    I. G. Meschersky

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  1. T. E. Kramina
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  2. G. V. Degtjareva
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  3. I. G. Meschersky
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Correspondence to T. E. Kramina.

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Kramina, T.E., Degtjareva, G.V. & Meschersky, I.G. Analysis of hybridization between tetraploid Lotus corniculatus and diploid Lotus stepposus (Fabaceae-Loteae): morphological and molecular aspects. Plant Syst Evol 298, 629–644 (2012). https://doi.org/10.1007/s00606-011-0572-6

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