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Genetic diversity of Cypripedium calceolus in Poland

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Abstract

In this work we assessed the genetic diversity of 32 C. calceolus populations from Poland. Mean genetic diversity was moderate (P = 36.4%, A = 1.58, H O = 0.143, F IS = 0.059), and seven geographic regions did not differ significantly in their levels of polymorphism (p > 0.05), although allele frequencies varied greatly. Only four unique alleles were found, at three sites in southern and southeastern Poland. Genetic (P, A) and genotypic diversity parameters (G, G U) were significantly correlated with population size (p < 0.001). In the 32 studied populations we separated 422 different multilocus genotypes; none was common to all populations. Overall population differentiation was moderate at 0.137 (p < 0.001), but we found a significant pattern of isolation by distance for the whole dataset (r 2 = 0.65, p < 0.001). Our chloroplast DNA (cpDNA) results suggest a single evolutionary lineage and a common origin for all Polish C. calceolus populations. Information about the genetic health of C. calceolus populations should be useful in developing conservation strategies.

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Acknowledgments

We thank Adam Chlebowski, Marek Cieślak, Paweł Frąckowiak, Adam Hermaniuk, Adam Kawiński, Róża Kaźmierczakowa, Ewa and Maciej Korczyńscy, Marek Kucharczyk, Jacek Łoziński, Julita Minasiewicz, Zbigniew Mirek, Józek Mitka, Andrzej Szlachetka, Marek Śleszyński, Iwona Wróbel, Zdzisław Zaborowski and Joanna Znaniecka for their help collecting material in the field. Michael Jacobs edited the manuscript. The study was done within project 2P04C 048 30 financed by the Polish Ministry of Science and Higher Education.

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  1. Institute of Biology, University of Białystok, Świerkowa 20B, 15-950, Białystok, Poland

    Emilia Brzosko, Ada Wróblewska, Izabela Tałałaj & Edyta Wasilewska

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  1. Emilia Brzosko
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Correspondence to Emilia Brzosko.

Appendix 1

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Table 5 Allele frequencies in 32 Cypripedium calceolus populations in Poland
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Brzosko, E., Wróblewska, A., Tałałaj, I. et al. Genetic diversity of Cypripedium calceolus in Poland. Plant Syst Evol 295, 83–96 (2011). https://doi.org/10.1007/s00606-011-0464-9

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