Skip to main content

Advertisement

SpringerLink
Log in

Genetic relationships among South American species of Cunila D. Royen ex L. based on ISSR

  • Original Article
  • Published:
Plant Systematics and Evolution Aims and scope Submit manuscript

Abstract

Cunila species are among the commonly used South Brazilian plants in popular medicine. This genus presents two centers of distribution in North and South America; the latter being classified into three sections: Incanae, Incisae and Spicatae. Based on the prospective utility as aromatic and medicinal plants, the aim of this work was to examine the genetic diversity among the South American species to contribute to the knowledge of their botanical sections. In this context, 11 Cunila species were analyzed by ISSR using seven primers that generated a total of 107 bands. The relationship was evaluated by constructing dendrograms using the UPGMA algorithm and analysis of principal components. The cluster analysis places the species C. origanoides with the South American species, but forming an independent cluster. Remarkably, among South American species two clusters emerge, one consisting of shrubs and the second by subshrubs species, which refines the botanical taxonomy for several species of the genus.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Agostini G (2003) Micropropagação, variabilidade química e genética em Cunila incisa Benth. Dissertação de mestrado. Curso de Pós-Graduação em Biotecnologia, Universidade de Caxias do Sul. Caxias do Sul, p 72

  • Ajibade SR, Weeden NF, Chite SM (2000) Inter simple sequence repeat analysis of genetic relationship in the genus Vigna. Euphytica 111:47–55

    Article  CAS  Google Scholar 

  • Albuquerque MR (2004) Avaliação de variabilidade química e genética em Cunila spicata Benth.. Dissertação de Mestrado. Curso de Pós-Graduação em Biotecnologia, Universidade de Caxias do Sul. Caxias do Sul, p 65

  • Bordignon SAL (1997) Estudo botânico e químico de espécies de Cunila Royen ex L. (Lamiaceae) nativas do sul do Brasil. Tese de doutorado. Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul. Porto Alegre, RS. p 197

  • Bordignon SAL, Schenkel EP, Spitzer V (1996) The essential oil of Cunila incisa (Lamiaceae). A rich source of 1, 8-cineole. Quím Nova 19:105–107

    CAS  Google Scholar 

  • Bordignon SAL, Schenkel EP, Spitzer V (1997) The essential oil composition of Cunila microcephala and Cunila fasciculata. Phytochemistry 44:1283–1286

    Article  CAS  Google Scholar 

  • Bordignon SAL, Schenkel EP, Spitzer V (1998) The essential oil of Cunila menthoides Bentham (Lamiaceae). J Essent Oil Res 10:317–320

    Google Scholar 

  • Bordignon SAL, Schenkel EP, Spitzer V (1999) The essential oil composition of Cunila angustifolia Bentham (Lamiaceae). J Essent Oil Res 11:145–148

    CAS  Google Scholar 

  • Bordignon SAL, Montanha JA, Schenkel EP (2003) Flavones and flavanones from South American Cunila species (Lamiaceae). Biochem Syst Ecol 31:785–788

    Article  Google Scholar 

  • Bornet B, Branchard M (2001) Nonanchored inter simple sequence repeat (ISSR) Markers: reproducible and specific tools for genome fingerprinting. Plant Mol Biol Rep 19:209–215

    Article  CAS  Google Scholar 

  • Botstein D, White RL, Skolnick M, Davis RW (1980) Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Amer J Hum Genet 32:314–331

    PubMed  CAS  Google Scholar 

  • Cantino PD, Harley RM, Wagstaff SJ (1992) Genera of labiate: status and classification. In: Harley RM, Reynolds T (Eds) Advances in labiate science. Royal Botanic Gardens, Kew, pp 511–522

    Google Scholar 

  • Doyle J, Doyle JL (1987) Isolation of plant DNA from fresh tissue. Amer J Bot 75:1238

    Article  Google Scholar 

  • Duke JA (1994) Biologically-active compounds in important spices. In: Charalambous (ed) Spices, herbs, and edible fungi. Elsevier Publishers, Amsterdam, p 595

  • Echeverrigaray S, Agostini G, Atti-Serfini L, Paroul N, Pauletti GF, Atti dos Santos AC (2001) Correlation between the chemical and genetic relationships among commercial thyme cultivars. J Agr Food Chem 49:4220–4223

    Article  CAS  Google Scholar 

  • Epling C (1936) Synopsis of the South American Labiatae. Repert Spec Nov Regni Veg Beih II 85:97–192

    Google Scholar 

  • Epling C (1951) Supplementary notes on American Labiatae-V. Brittonia 7:129–142

    Article  Google Scholar 

  • Esselman EJ, Jianqiang L, Crawford DJ, Winduss JL, Wolfe AD (1999) Clonal diversity in the rare Calamagrostis porteri ssp. Insperata (Poaceae): comparative results for allozymes and random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) markers. Molec Ecol 8:443–451

    Article  CAS  Google Scholar 

  • Fang DQ, Roose ML (1997) Identication of closely related citrus cultivars with inter-simple sequence repeat markers. Theor Appl Genet 95:408–417

    Article  CAS  Google Scholar 

  • Fracaro F, Echeverrigaray S (2006) Genetic variability in Hesperozygis ringens Benth. (Lamiaceae) an endangered aromatic and medicinal plant of southern Brazil. Biochem Genet 44:11–12

    Article  Google Scholar 

  • Fracaro F, Zacaria J, Echeverrigaray S (2005) RAPD based genetic relationships between populations of three chemotypes of Cunila galioides Benth. Biochem Syst Ecol 33:409–417

    Article  CAS  Google Scholar 

  • Garcia-Peña MR (1989) A new species of Cunila (Lamiaceae) from southwestern Mexico. Kew Bull 44:727–730

    Article  Google Scholar 

  • Hunter PR, Gaston MA (1988) Numerical index of the discriminatory ability of typing systems: an application of Simpson´s index of diversity. J Clin Microbiol 26:2465–2466

    PubMed  CAS  Google Scholar 

  • Kimball RT, Crawford DJ, Page JR, Harmon PJ (2001) Inter-simple sequence repeat (ISSR) diversity within Monarda fistulosa var. brevis (Lamiaceae) and divergence between var. brevis and var. fistulosa in West Virginia. Brittonia 53:511–518

    Google Scholar 

  • Kochieva EZ, Ryzhova NN, Legkobit MP, Khadeeva NV (2006) RAPD and ISSR analysis of species and populations of the genus Stachys. Russ J Genet 42:723–727

    Article  CAS  Google Scholar 

  • Liu J, Wang L, Geng Y, Wang Q, Luo L, Zhong Y (2006) Genetic diversity and population structure of Lamiophlomis rotate (Lamiaceae), an endemic species of Qinghai-Tibet Plateau. Genetica 128:385–394

    Article  PubMed  Google Scholar 

  • Peakall R, Smouse PE (2001) GenALEx V5: Genetic analysis in excel. Population genetic software for teaching and research. Australian National University, Canberra

  • Reddy MP, Sarla N, Siddiq EA (2002) Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding. Euphytica 128:9–17

    Article  Google Scholar 

  • Rohlf FJ (2001) NTSYSpc: numerical taxonomy and multivariate analysis system, version 2.1. Exeter Software, Setauket, New York

  • Shi S, Qiu Y, Wu L, Fu C (2006) Interspecific relationships of Lycoris (Amaryllidaceae) inferred from inter-simple sequence repeat data. Sci Hortic 110:285–291

    Article  CAS  Google Scholar 

  • Simões CMO, Mentz LA, Schenkel EP, Irgang BE, Stehmann JR (1994) Plantas da medicina popular no Rio Grande do Sul, 4th ed., Editora da Universidade/UFRGS, Porto Alegre, Brazil

  • Skoula M, El Hilali I, Makris AM (1999) Evaluation of the genetic diversity of Salvia fruticosa Mill. clones using RAPD markers and comparison with the essential oil profiles. Biochem Syst Ecol 27:559–568

    Article  CAS  Google Scholar 

  • Sudupak ME (2004) Inter and intra-species inter simple sequence repeat (ISSR) variations in the genus Cicer. Euphytica 135:229–238

    Article  CAS  Google Scholar 

  • Valk HA, Meis JFGM, Curfs IM, Muehlethaler K, Mouton JW, Klaassen CHW (2005) Use of a novel panel of nine short tandem repeats for exact and high-resolution fingerprinting of Aspergillus fumigatus isolates. J Clin Microbiol 43:4112–4120

    Article  PubMed  Google Scholar 

  • Yap I, Nelson RJ (1996) Winboot: a program for performing bootstrap analysis of binary data to determine the confidence limits of UPGMA based dendograms. International Rice Research Institute, Manila, Discussion Paper series 14

  • Zietkiewicz E, Rafalski A, Labuda D (1994) Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain-reaction amplification. Genome 20:176–183

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors are grateful to the Universidade Federal do Rio Grande do Sul for financial support and provision of its facilities, and CAPES for the doctoral fellowship provided to G. Agostini. We wish to thank Dr George Weiblen, Curator of the University of Minnesota Herbarium, for the kind donation of samples of C. origanoides, and Dr Sérgio A. de L. Bordignon, ULBRA and UNILASSALE, for the identification of Cunila species and for his invaluable assistance during field expeditions.

Author information

Authors and Affiliations

  1. Programa de Pós-Graduação em Botânica, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil

    G. Agostini

  2. Instituto de Biotecnologia, Universidade de Caxias do Sul, R. Francisco Getúlio Vargas, 1130, Petrópolis, PO Box 1352, Caxias do Sul, RS, CEP 95001-970, Brazil

    S. Echeverrigaray

  3. Departamento de Botânica, Universidade Federal do Rio Grande do Sul, Prédio 43433, Porto Alegre, RS, CEP 91501-970, Brazil

    T. T. Souza-Chies

Authors
  1. G. Agostini
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. Echeverrigaray
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. T. T. Souza-Chies
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to T. T. Souza-Chies.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Agostini, G., Echeverrigaray, S. & Souza-Chies, T.T. Genetic relationships among South American species of Cunila D. Royen ex L. based on ISSR. Plant Syst Evol 274, 135–141 (2008). https://doi.org/10.1007/s00606-008-0037-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00606-008-0037-8

Keywords

Search

Navigation