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Analysis of the genetic diversity and relationships among and within species of Hippophae (Elaeagnaceae) based on RAPD markers

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Abstract

RAPD markers were used to assess the genetic diversity and inter- and intra-specific relationships of the genus Hippophae L. and to study the correlation between genetic distances and geographic distances among populations of H. rhamnoides ssp. sinensis. The results analyzed by the percentage of polymorphic loci and Shannon information index indicated that a high level of genetic diversity existed both among and within species of the genus Hippophae. In the UPGMA dendrogram, the species or subspecies were clustered into two main groups but not strictly grouped according to sect. Hippophae and sect. Gyantsensis Lian. The multiple regression analysis and Mantel test both indicated a significant correlation between genetic distance and altitude distance among populations of H. rhamnoides ssp. sinensis, and the cluster analysis suggested that the genetic variation among populations of H. rhamnoides ssp. sinensis was linked to their monophyletic origin. Moreover, some degree of genetic differentiation was found among samples collected at different times.

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References

  • T. R. Allnutt A. C. Newton A. Premoli (2003) ArticleTitleGenetic variation in the threatened South American conifer Pilgerodendron uviferum (Cupressaceae), detected using RAPD markers Biological Conservation 114 245–253 Occurrence Handle10.1016/S0006-3207(03)00044-2

    Article  Google Scholar 

  • P. Alpert R. D. Lumaret F. Giusto (1993) ArticleTitlePopulation structure inferred from allozyme analysis in the clone herb Fragaria chiloensis (Rosaceae) Amer. J. Bot. 80 1002–1006 Occurrence Handle1:CAS:528:DyaK2cXht12lu7o%3D Occurrence Handle10.2307/2445746

    Article  CAS  Google Scholar 

  • V. I. Avdeyev (1983) ArticleTitleNovaya taksonomiya roda oblepikha: Hippophae L. Izv. Akad. Nauk. Tadzh. SSR Biol. Nauk. 4 11–17

    Google Scholar 

  • I. V. Bartish N. Jeppsson H. Nybom (1999) ArticleTitlePopulation genetic structure in the dioecious pioneer plant species Hippophae rhamnoides investigated by random amplified polymorphic DNA (RAPD) markers Molec. Ecol. 8 791–802 Occurrence Handle1:CAS:528:DyaK1MXktVaqsbg%3D Occurrence Handle10.1046/j.1365-294X.1999.00631.x

    Article  CAS  Google Scholar 

  • I. V. Bartish N. Jeppsson G. I. Bartish R. Lu H. Nybom (2000) ArticleTitleInter- and intraspecic genetic variation in Hippophae (Elaeagnaceae) investigated by RAPD markers Pl. Syst. Evol. 225 85–101 Occurrence Handle1:CAS:528:DC%2BD3MXhsFGjs74%3D Occurrence Handle10.1007/BF00985460

    Article  CAS  Google Scholar 

  • I. V. Bartish N. Jeppsson H. Nybom U. Swenson (2002) ArticleTitlePhylogeny of Hippophae (Elaeagnaceae) inferred from parsimony analysis of choloroplast DNA and morphology Syst. Bot. 27 IssueID1 41–54

    Google Scholar 

  • D. E. Butos C. Casanova C. Soler N. Jouve (1998) ArticleTitleRAPD variation in wild populations of four species of the genus Hordeum (Poaceae) Theor. Appl. Genet. 96 101–111 Occurrence Handle10.1007/s001220050715

    Article  Google Scholar 

  • X. L. Chen Y. S. Lian (1994) ArticleTitleThe geographical distribution pattern and its formative factors on the genus Hippophae L Acta Bot. Boreal.-Occident. Sin. 14 IssueID6 105–110 Occurrence Handle1:CAS:528:DyaK2MXit1ClsL4%3D

    CAS  Google Scholar 

  • X. L. Chen R. J. Ma K. Sun Y. S. Lian (2003) ArticleTitleGermplasm resource and habitat types of Seabuckthorn in China Acta Bot. Boreal.-Occident. Sin. 23 IssueID3 451–455 Occurrence Handle1:CAS:528:DC%2BD2cXlsVCgsL0%3D

    CAS  Google Scholar 

  • R. Durand B. Durand (1990) ArticleTitleSexual determination and sexual differentiation Crit. Rev. Pl. Sci. 9 295–316 Occurrence Handle10.1080/07352689009382292

    Article  Google Scholar 

  • Q. P. Gu (2003) ArticleTitleExploitation and utilization of Hippophae L Hippophae 16 IssueID2 40–42

    Google Scholar 

  • J. Hyvonen (1996) ArticleTitleOn phylogeny of Hippophae (Elaeagnaceae) Nord. J. Bot. 16 51–62

    Google Scholar 

  • E. E. Irish T. Nelson (1989) ArticleTitleSex determination in monoecious and dioecious plants Plant Cell 1 737–744 Occurrence Handle12359907 Occurrence Handle10.1105/tpc.1.8.737

    Article  PubMed  Google Scholar 

  • K. Z. Jin H. Yin Y. C. Yan (1995) ArticleTitleDevelopment and structure of root nodules and endophytes Frankia in the Chinese Sea Buckthorn (Hippophae rhamnoides subsp sinensis) Journal of Northwest University (Natural science Edition) 25 IssueID2 155–170

    Google Scholar 

  • X. J. Jin (2002) ArticleTitleAnalysis of effective composition of Hippophae rhamnoides L Chem. Res. Chinese U. 18 IssueID3 274–276

    Google Scholar 

  • S. Kumar K. Tamura B. I. Jakobsen M. Nei (2001) ArticleTitleMEGA2: Molecular Evolutionary Genetics Analysis Software Bioinformatics 17 1244–1245 Occurrence Handle11751241 Occurrence Handle1:CAS:528:DC%2BD38XmtVCktQ%3D%3D Occurrence Handle10.1093/bioinformatics/17.12.1244

    Article  PubMed  CAS  Google Scholar 

  • R. C. Lewontin (1972) ArticleTitleApportionment of human diversity Evol. Biol. 6 381–398

    Google Scholar 

  • F. Li Z. T. Xiong F. M. Li Y. G. Zhu (1999) ArticleTitleGenetic diversity and divergence between populations of Hemerocallis lilioasphodelus L. from Henan and Hunan Province J. Wuhan Univ. (Nat. Sci. Ed.) 45 849–851 Occurrence Handle1:CAS:528:DC%2BD3cXhtVCqs7o%3D

    CAS  Google Scholar 

  • G. Q. Li D. R. Tang Y. Q. Zhao (2000) ArticleTitleThe biological property and ecological habit of Hippophae Acta Bot. Boreal-Occident. Sin. 20 IssueID5 892–897

    Google Scholar 

  • J. Li Y. Tao S. Z. Zheng J. L. Zhou (1995) ArticleTitleIsozymatic differentiation in local population of Glycine soja Sieb. & Zucc Acta Bot. Sin. 37 669–676 Occurrence Handle1:CAS:528:DyaK28XhsVOgs7g%3D

    CAS  Google Scholar 

  • Y. S. Lian (1988) ArticleTitleNew discoveries of the genus Hippophae (Elaeagnaceae) Acta. Phytotax. Sin. 26 235–237

    Google Scholar 

  • Y. S. Lian X. L. Chen R. J. Ma C. B. Li (1998) ArticleTitleThe primary study of biological and ecological property for the genus Hippophae L Hippophae 11 IssueID3 1–7

    Google Scholar 

  • Y. S. Lian X. L Chen F. Wang (1997) ArticleTitleInvestigation of intraspecific patterns within Hippophae rhamnoides subsp sinensis Journal of Northwest Normal University (Natural Science Edition). 33 IssueID1 36–46

    Google Scholar 

  • Y. S. Lian X. L. Chen (1992) ArticleTitleThe ecogeographical distribution of Hippophae rhamnoides subsp sinensisand its phytogeographical significance Acta Phytotax. Sin. 30 IssueID2 394–355

    Google Scholar 

  • Y. S. Lian X. L. Chen (1996) ArticleTitleSystematic classification of the genus Hippophae L Hippophae 9 IssueID1 15–24

    Google Scholar 

  • Y. S. Lian X. L. Chen (2000) ArticleTitleThe regular patterns of distribution on the natural components in plants of the genus Hippophae L Journal of Northwest Normal University (Natural Science Edition) 36 IssueID1 113–128 Occurrence Handle1:CAS:528:DC%2BD3cXhsl2ntbc%3D

    CAS  Google Scholar 

  • Y. S. Lian X. L. Chen K. Sun (2003) ArticleTitleA new subspecies of Hippophae (Elaeagnaceae) from China Novon 13 200–202

    Google Scholar 

  • Y. S. Lian X. L. Chen Z. D. Yu F. Ao (1997) ArticleTitleStudy on the origin of the genus Hippophae L Hippophae 10 IssueID2 1–7

    Google Scholar 

  • Lian Y. S., Lu S. G., Xue S. K., Chen X. L. (2000) Biology and chemistry of the genus Hippophae. Lanzhou: Gansu science & Technology Press, pp. 1–226. (in Chinese).

  • M. Nei (1973) ArticleTitleAnalysis of gene diversity in subdivided populations Proc. Natl. Acad. Sci. USA 70 3321–3323 Occurrence Handle4519626 Occurrence Handle1:STN:280:DyaE2c%2FlsFCrtQ%3D%3D Occurrence Handle10.1073/pnas.70.12.3321

    Article  PubMed  CAS  Google Scholar 

  • H. A. Persson H. Nybom (1998) ArticleTitleGenetic sex determination and RAPD marker segregation in the dioecious species sea buckthorn (Hippophae rhamnoides L.) Hereditas 129 45–51 Occurrence Handle1:CAS:528:DyaK1MXkslCk Occurrence Handle10.1111/j.1601-5223.1998.00045.x

    Article  CAS  Google Scholar 

  • A. Rousi (1971) ArticleTitleThe genus Hippophae L. A taxonomic study Ann. Bot. Fennici 8 177–227

    Google Scholar 

  • A. Schnabel J. L. Hamrick (1990) ArticleTitleOrganization of genetic diversity within and among populations of Gleditsia triacanthos (Leguminosae) Amer. J. Bot. 77 1060–1069 Occurrence Handle10.2307/2444577

    Article  Google Scholar 

  • K. Sun X. L. Chen R. Ma C. Li Q. Wang S. Ge (2002) ArticleTitleMolecular phylogenetics of Hippophae L (Elaeagnaceae) based on the internal transcribed spacer (ITS) sequences of nrDNA Pl. Syst. Evol. 235 121–134 Occurrence Handle1:CAS:528:DC%2BD38Xptleht7g%3D Occurrence Handle10.1007/s00606-002-0206-0

    Article  CAS  Google Scholar 

  • K. Sun X. L. Chen R. Ma Y. L. Liu (1997) ArticleTitleStudy on the speciation of Hippophae goniocarpa Journal of Northwest Normal University (Natural Science Edition) 33 IssueID4 42–51

    Google Scholar 

  • D. R. Tang Y. Q. Zhao (2000) ArticleTitleThe resource and the exploitation and utilization of Hippophae L Hippophae 13 IssueID2 22–29

    Google Scholar 

  • C. J. Tian Y. D Lei S. H. Shi P. Nan J. K. Chen Y. Zhong (2004) ArticleTitleGenetic diversity of Sea buckthorn (Hippophae rhamnoides) populations in northeastern and northwestern China as revealed by ISSR markers New Forest. 27 229–237 Occurrence Handle10.1023/B:NEFO.0000022224.59436.7a

    Article  Google Scholar 

  • Wang Q. (2003) Genetic diversity of three species of Ligularia (Asteraceae) in eastern of Qinghai-Tibet plateau of China. M.D. Thesis, pp. 14–19.

  • X. M. Wang W. Q. Song X. L. Li R. Y. Chen (2002) ArticleTitleThe analysis of DNA difference between the genome of male and female in Ginkgo biloba L Chin. J. Cell. Biol. 24 IssueID1 38–40

    Google Scholar 

  • Y. L. Wang G. Q. Yan L. Xu S. Li L. J. Zhang G. F. Zhao (2003) ArticleTitleGenetic structure and differentiation of Petrosimonia sibirica populations in the desert of Fukang, Xinjiang Acta. Bot. Boreal. -Occident. Sin. 23 IssueID5 700–704 Occurrence Handle1:CAS:528:DC%2BD2MXhsFCmtLs%3D

    CAS  Google Scholar 

  • Weising K., Nybom H., Wolf K., Meyer W. (1995) DNA fingerprinting in plants and fungi. CRC Press. Inc. Boca. Raton, pp. 159–200.

  • Whitkus R., Doebley J., Wendel J. F. (1994) Nuclear DNA markers in systemtics and evolution. In: Phillips R. L., Vasil I. K. (eds.) DNA based markers in plants. Kluwer Academic Publishers, pp. 116–141.

  • J. G. K. Williams A. R. Kubelik K. J. Livak J. A. Rafalski S. V. Tingey (1990) ArticleTitleDNA polymorphisms amplified by arbitrary primers are useful genetic markers Nucleic Acids Res 18 6531–6535 Occurrence Handle1979162 Occurrence Handle1:CAS:528:DyaK3MXjslWmsA%3D%3D

    PubMed  CAS  Google Scholar 

  • L. Wu Y. Huo X. L. Nie L. J. Wang (2003) ArticleTitleStudies on leaf tissue parameters and their relations to drought resistance of Sea Buckthorn Journal of Jilin Agricultural University 25 IssueID4 390–393

    Google Scholar 

  • Yeh F. C., Yang R. C. (1999) POPGENE V 1.31. University of Alberta and Center for International Research.

  • Z. D. Yu F. Ao Y. S. Lian (1993) ArticleTitleThe origin, classfication, populations and resource of the genus Hippophae L. in China Hippophae 6 IssueID1 19–24

    Google Scholar 

  • L. H. Zhao G. H. Huang X. L. Nie H. Z. Liu (2002) ArticleTitleResistance and morphological studies on differ Hippophae sps Journal of Northeast Normal University 34 IssueID4 70–74

    Google Scholar 

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Authors and Affiliations

  1. School of Life Science, Key Laboratory of Arid and Grassland Agroecology, Lanzhou University, Ministry of Education, Lanzhou, 730000, China

    H. M. Sheng, L. Z. An, S. J. Xu, X. L. Zheng, L. L. Pu & Y. J. Liu

  2. Cold and Arid Regions Environmental and Engineering Research Institute, CAS, Lanzhou, 730000, China

    L. Z. An, T. Chen & G. X. Liu

  3. School of Life Sciences, North-west Normal University, Lanzhou, China

    Y. S. Lian

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  1. H. M. Sheng
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  2. L. Z. An
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  4. S. J. Xu
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Correspondence to L. Z. An.

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Sheng, H.M., An, L.Z., Chen, T. et al. Analysis of the genetic diversity and relationships among and within species of Hippophae (Elaeagnaceae) based on RAPD markers. Plant Syst. Evol. 260, 25–37 (2006). https://doi.org/10.1007/s00606-006-0413-1

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