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Multiorbital DNA walker nanoprobe for portable photothermal detection based on H2S etching of cubic Prussian blue

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Abstract

In the developed assay, multiorbital 3D DNA walker (MO DNA walker) was applied as signal amplified protocol for enhancing the detection signal of the photothermal biosensor, which was designed for sensitive detection of miRNA based on the H2S triggered conversation of photothermal reagent. When the target molecule is present, the DNA walking strand was released and then hybridize with track strands. The landing of walking particles (WPT) on the tracking particles (TPT) promotes the relative movement of the WPT around TPT, thus releasing large amount of horseradish peroxidase (HRP) with the aid of DNAzyme. After reacting with Na2S2O3 and H2O2, multiple H2S can be generated in situ based on the catalytic ability of HRP. Meanwhile, cubic Prussian blue (CPB) was synthesized and exhibited superior photothermal response, which can be served as efficient photothermal reagent and H2S responsive acceptor. Significantly, the photothermal signal of CPB could be obviously reduced after challenged with H2S ascribed to synchronous reaction between the ferric ion (Fe3+) and H2S. The improved walking area and freedom enable significant signal amplification, enhancing the biosensor’s performance. Under ideal circumstances, the proposed photothermal assay demonstrated excellent performance for determination of miRNA-21.

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Funding

This work was financially supported by the National Natural Science Foundation of China (22366019&21864013) and the Natural Science Foundation of Jiangxi Province (20232ACB213011&20212BCJL23057).

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Authors and Affiliations

  1. Key Lab of Fluorine and Silicon for Energy Materials and Chemistry of Ministry of Education, Key Laboratory for Green Chemistry of Jiangxi Province, Department of Chemistry and Chemical Engineering, National Engineering Research Center for Carbohydrate Synthesis, Jiangxi Normal University, Nanchang, 330022, People’s Republic of China

    Jinjin Li, Jiao Qin, Fan Du, Wenqin Meng, Yunhong Huang & Juan Tang

  2. Key Laboratory of Analysis and Detection for Food Safety (Ministry of Education of China and Fujian Province), Department of Chemistry, Fuzhou University, Fuzhou, 350108, People’s Republic of China

    Dianping Tang

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  1. Jinjin Li
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Correspondence to Yunhong Huang or Juan Tang.

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Li, J., Qin, J., Du, F. et al. Multiorbital DNA walker nanoprobe for portable photothermal detection based on H2S etching of cubic Prussian blue. Microchim Acta 190, 382 (2023). https://doi.org/10.1007/s00604-023-05957-y

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