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Label-free MXene-assisted field effect transistor for the determination of IL-6 in patients with kidney transplantation infection

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Abstract

A spiral interdigitated MXene–assisted field effect transistor (SiMFETs) was proposed for determination of IL-6 in patients with kidney transplantation infection. Our SiMFETs demonstrated enhanced IL-6 detection range of 10 fg/mL–100 ng/mL due to the combination of optimized transistor’s structure and semiconducting nanocomposites. Specifically, on one hand, MXene-based field effect transistor drastically amplified the amperometric signal for determination of IL-6; on the other hand, the multiple spiral structure of interdigitated drain-source architecture improved the transconductance of FET biosensor. The developed SiMFETs biosensor demonstrated satisfactory stability for 2 months, and favorable reproducibility and selectivity against other biochemical interferences. The SiMFETs biosensor exhibited acceptable correlation coefficient (R2=0.955) in quantification of clinical biosamples. The sensor successfully distinguished the infected patients from the health control with enhanced AUC of 0.939 (sensitivity of 91.7%, specificity of 86.7%). Those merits introduced here may pave an alternative strategy for transistor-based biosensor in point-of-care clinic applications.

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Funding

This research was funded by the Natural Science Foundation of China (81800657, 81770748 and 81900680).

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Authors and Affiliations

  1. Department of Urology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China

    Dawei Li, Yaofei Ren, Ruoyang Chen, Haoyu Wu, Shaoyong Zhuang & Ming Zhang

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  1. Dawei Li
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  2. Yaofei Ren
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  3. Ruoyang Chen
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  4. Haoyu Wu
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  5. Shaoyong Zhuang
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  6. Ming Zhang
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Contributions

Dawei Li: conceptualization, methodology, investigation, writing-original draft, formal analysis. Yaofei Ren: investigation, writing-original draft. Ruoyang Chen: writing-original draft. Haoyu Wu: software. Shaoyong Zhuang: characterizations. Ming Zhang: supervision, writing—review and editing. Dawei li and Yaofei Ren contributed equally this work.

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Correspondence to Ming Zhang.

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Li, D., Ren, Y., Chen, R. et al. Label-free MXene-assisted field effect transistor for the determination of IL-6 in patients with kidney transplantation infection. Microchim Acta 190, 284 (2023). https://doi.org/10.1007/s00604-023-05814-y

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