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Cu2O@PdAg-quenched CdS@CeO2 heterostructure electrochemiluminescence immunosensor for determination of prostate-specific antigen

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Abstract

Based on the resonance energy transfer between CdS@CeO2 and Cu2O@PdAg, a quenching immunosensor for sensitive detection of prostate specific antigen (PSA) was constructed. The CdS@CeO2 heterostructure was obtained by in situ growth of CeO2 particles on the surface of CdS nanorods, and stable cathodic ECL emission was achieved using K2S2O8 as coreactant. Cu2O@PdAg was composed of Cu2O with tetradecahedral structure and bimetallic PdAg nanospheres and has a UV–V is absorption range between 600 and 800 nm. It overlaps with the ECL emission spectrum of CdS@CeO2, realizing the effective quenching of the ECL signal, which provides feasibility for subsequent practical application. The immunosensor exhibited good linearity in the concentration range 10 fg·mL−1 ~ 100 ng·mL−1, with a detection limit of 5.6 fg·mL−1. In sample analysis, the recoveries were 99.8–101%, and the relative standard deviation (RSD) was 0.85–1.6% showing great potential and development value for the sensitive detection of prostate cancer.

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Funding

This research was financially supported by the Innovation Team Project of Colleges and Universities in Jinan (No. 2019GXRC027), the National Natural Science Foundation of China (Nos. 22274063, 51904114, 21777056, 21675063), and the Special Foundation for Taishan Scholar Professorship of Shandong Province.

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  1. Zhengxing Gong and Xinrong Shao contributed equally to this work.

Authors and Affiliations

  1. Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, People’s Republic of China

    Zhengxing Gong, Xinrong Shao, Jing Luo, Xu Sun, Hongmin Ma, Dan Wu, Dawei Fan, Yuyang Li, Qin Wei & Huangxian Ju

  2. Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, People’s Republic of China

    Zhengxing Gong, Xinrong Shao, Jing Luo, Xu Sun, Hongmin Ma, Dan Wu, Dawei Fan, Yuyang Li, Qin Wei & Huangxian Ju

  3. State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, People’s Republic of China

    Huangxian Ju

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  1. Zhengxing Gong
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Gong, Z., Shao, X., Luo, J. et al. Cu2O@PdAg-quenched CdS@CeO2 heterostructure electrochemiluminescence immunosensor for determination of prostate-specific antigen. Microchim Acta 190, 59 (2023). https://doi.org/10.1007/s00604-023-05635-z

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