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Dual-mode photoelectrochemical/electrochemical sensor based on Z-scheme AgBr/AgI-Ag-CNTs and aptamer structure switch for the determination of kanamycin

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Abstract

A “signal-on” dual-mode aptasensor based on photoelectrochemical (PEC) and electrochemical (EC) signals was established for kanamycin (Kana) assay by using a novel Z-scheme AgBr/AgI-Ag-CNTs composite as sensing platform, an aptamer structure switch, and K3[Fe(CN)6] as photoelectron acceptor and electrochemical signal indicator. The aptamer structure switch was designed to obtain a “signal-off” state, which included an extended Kana aptamer (APT), one immobilized probe (P1), and one blocking probe (P2) covalently linked with graphdiyne oxide (GDYO) nanosheets. P1, P2, and aptamer formed the double helix structure, which resulted in the inhibited photocurrent intensity because of the weak conductivity of double helix layer and serious electrostatic repulsion of GDYO towards K3[Fe(CN)6]. In the presence of Kana, APT specifically bound to the target and dissociated from P1 and P2, and thus, a “signal-on” state was initiated by releasing P2-GDYO from the platform. Based on the sensing platform and the aptamer structure switch, the dual-mode aptasensor realized the linear determination ranges of 1.0 pM–2.0 μM with a detection limit (LOD) of 0.4 pM (for PEC method) and 10 pM–5.0 μM with a LOD of 5 pM (for EC method). The aptasensor displayed good application potential for Kana test in real samples.

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Funding

This research was supported by the Science and Technology Innovation Program of Hunan Province (2021RC5028), Scientific Research Fund of Hunan Provincial Education Department (21A0295), the Outstanding Youth Foundation of Hunan Province (2020JJ2014), and the Research and Development Planning Projects in Key Areas of Hunan Province (No. 2019GK2034).

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Authors and Affiliations

  1. Key Laboratory of Theoretical Organic Chemistry and Function Molecule, Ministry of Education, Hunan University of Science and Technology, Xiangtan, 411201, People’s Republic of China

    Zhang Liu, Keqin Deng, Jinglun Wang & Haowen Huang

  2. Hunan Province College Key Laboratory of Molecular Design and Green Chemistry, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan, 411201, China

    Zhang Liu, Keqin Deng, Heng Zhang, Chunxiang Li, Qingfeng Yi & Hu Zhou

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  1. Zhang Liu
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  2. Keqin Deng
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Correspondence to Keqin Deng.

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Liu, Z., Deng, K., Zhang, H. et al. Dual-mode photoelectrochemical/electrochemical sensor based on Z-scheme AgBr/AgI-Ag-CNTs and aptamer structure switch for the determination of kanamycin. Microchim Acta 189, 417 (2022). https://doi.org/10.1007/s00604-022-05523-y

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