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Coupled electrochemiluminescent and resonance energy transfer determination of microRNA-141 using functionalized Mxene composite

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Abstract

The electrochemiluminescence and resonance energy transfer (ECL-RET) method was adopted to detect miRNAs, in which the two-dimensional Ti3C2 Mxenes with high surface area modified with CdS:W nanocrystals (CdS:W NCs) were used as ECL signal emitter. Mxenes with a specific surface area of 5.2755 m2/g carried more emitters and promote ECL intensity. As an energy acceptor, BiOCl nanosheets (BiOCl NSs) have a wide UV–Vis absorption peak in the range 250 nm–700 nm, including the emission band of CdS:W NCs with 520 nm emission wavelength. Hence, BiOCl NSs are covalently bound to hairpin DNA 2 by amide bond to quench the ECL signal of CdS:W NCs. In the presence of miRNA-141, the hairpin DNA 1 modified on the GCE was unfold and then paired with hairpin DNA 2 to release miRNA-141 and quench the signal of the ECL biosensor. Then, the concentration signal of miRNA-141 was amplified by catalytic hairpin assembly. The novel specific biosensor demonstrated a satisfactory linear relationship with miRNA-141 in the range 0.6 pM to 4000 pM; the detection limit was as low as 0.26 pM (3 s/m) under the potential of 0 ~ -1.3 V and showed outstanding RSD of 1.19%. The findings of the present work with high accuracy and sensitivity will be of positive significance for the clinical diagnosis of miRNA in the future work.

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The construction process of the biosensor and electrochemiluminescence mechanism

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Acknowledgements

This work was supported by National Natural Science Foundation of China (21976001). Natural Science Foundation of Anhui Province (1508085MB37). University Collaborative Innovation Project of Anhui Province (GXXT-2019-023). Natural Science Research Projects of Universities in Anhui Province (KJ2021A0030). Open fund for Discipline Construction of Institute of Physical Science and Information Technology.

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Authors and Affiliations

  1. Key Laboratory of Structure and Functional Regulation of Hybrid Materials (Ministry of Education), School of Chemistry and Chemical Engineering, Anhui University, Hefei, 230601, Anhui, China

    Jin-Feng Du, Jing-Shuai Chen, Xing-Pei Liu, Chang-Jie Mao & Bao-Kang Jin

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Jin-Feng Du was involved in conceptualization, investigation, writing—original draft. Jing-Shuai Chen helped in methodology, investigation. Xing-Pei Liu contributed to validation. Chang-Jie Mao was involved in supervision, writing—review & editing. Bao-Kang Jin helped in writing—review & editing.

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Correspondence to Xing-Pei Liu or Chang-Jie Mao.

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Du, JF., Chen, JS., Liu, XP. et al. Coupled electrochemiluminescent and resonance energy transfer determination of microRNA-141 using functionalized Mxene composite. Microchim Acta 189, 264 (2022). https://doi.org/10.1007/s00604-022-05359-6

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