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Layer-by-layer assembly of multilayered double hydroxides/polyoxometalate-coated magnetic nanoparticles for highly efficient phosphopeptide enrichment

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Abstract

A layer-by-layer (LbL) assembly strategy was developed to prepare multilayered double hydroxide/polyoxometalate shell–coated magnetic nanoparticles. The introduction of functional shells not only offered abundant affinity sites of metal oxide and metal ions but also increased the surface area for the contact with targets. By combining the enrichment strategies of immobilized metal ion affinity chromatography and metal oxide affinity chromatography, the nanomaterial can capture phosphopeptides via a synergistic effect. The method presented a low detection limit of 0.1 fmol in combination with matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) analysis. The nanomaterial showed satisfactory selectivity (1:1:5000 M ratio of α-/β-casein/bovine serum albumin), good recovery (92.07%), high adsorption capacity (117.6 mg g−1), and ten times reusability for capturing phosphopeptides.

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Acknowledgements

We thank LetPub (www.letpub.com) for its linguistic assistance during the preparation of this manuscript.

Funding

This work was financially supported by the National Natural Science Foundation of China (22004073, 21961024 and 21961025), Natural Science Foundation of Inner Mongolia (2020BS02010 and 2020BS02015), Talent Research Support Funds from Government-Sponsored Institution of Inner Mongolia (RCQD19002), Inner Mongolia Minzu University Doctoral Research Startup Fund Project (BS516 and BS513), and Incentive Funding from Nano Innovation Institute (NII) of Inner Mongolia Minzu University.

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Authors and Affiliations

  1. College of Chemistry and Materials Science, Inner Mongolia Key Laboratory of Carbon Nanomaterials, Nano Innovation Institute (NII), Inner Mongolia Minzu University, Tongliao, 028000, People’s Republic of China

    Dandan Jiang, Siqi Lv, RuiXue Qi, Xue Han, Limei Duan & Jinghai Liu

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  1. Dandan Jiang
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Correspondence to Dandan Jiang.

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Jiang, D., Lv, S., Qi, R. et al. Layer-by-layer assembly of multilayered double hydroxides/polyoxometalate-coated magnetic nanoparticles for highly efficient phosphopeptide enrichment. Microchim Acta 189, 156 (2022). https://doi.org/10.1007/s00604-022-05258-w

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