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A competitive immunoassay for detecting triazophos based on fluorescent catalytic hairpin self-assembly

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A Correction to this article was published on 24 May 2022

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Abstract

A rapid detection method is introduced for residual trace levels of triazophos in water and agricultural products using an immunoassay based on catalytic hairpin self-assembly (CHA). The gold nanoparticle (AuNPs) surface was modified with triazophos antibody and sulfhydryl bio-barcode, and an immune competition reaction system was established between triazophos and its ovalbumin-hapten (OVA-hapten). The bio-barcode served as a catalyst to continuously induce the CHA reaction to achieve the dual signal amplification. The method does not rely on the participation of enzymes, and the addition of fluorescent materials in the last step avoids interfering factors, such as a fluorescence burst. The emitted fluorescence was detected at 489/521 nm excitation/emission wavelengths. The detection range of the developed method was 0.01–50 ng/mL for triazophos, and the limit of detection (LOD) was 0.0048 ng/mL. The developed method correlates well with the results obtained by LC–MS/MS, with satisfactory recovery and sensitivity. In sum, the designed method is reliable and provides a new approach to detect pesticide residues rapidly and quantitatively.

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modified by nanogold using colorimetric and UV spectrophotometric methods

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Funding

This study was financially supported by the National Key Research Program of China (No. 2019YFC1604503), NIEHS Superfund Research Program (No. P42 ES04699), Agricultural Science and Technology Innovation Program of CAAS (No. CAAS-ZDRW202011), and Ningbo Innovation Project for Agro-Products Quality and Safety (No. 2019CXGC007).

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Authors and Affiliations

  1. Institute of Quality Standard and Testing Technology for Agro-Products, Key Laboratory of Agro-Product Quality and Safety, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

    Yuanshang Wang, Ge Chen, Xueyan Cui, Lingyuan Xu, Zhen Cao, Yongxin She, Fen Jin, Yudan Zhang, Jing Wang, LuFei Zheng & Maojun Jin

  2. Key Laboratory of Agro-Product Quality and Safety, Ministry of Agriculture, Beijing, 100081, China

    Yuanshang Wang, Ge Chen, Xueyan Cui, Lingyuan Xu, Zhen Cao, Yongxin She, Fen Jin, Yudan Zhang, Jing Wang, LuFei Zheng & Maojun Jin

  3. State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan, 250353, China

    A. M. Abd El-Aty

  4. Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt

    A. M. Abd El-Aty

  5. Department of Medical Pharmacology, Medical Faculty, Ataturk University, 25240, Erzurum, Turkey

    A. M. Abd El-Aty & Ahmet Hacimuftuoglu

  6. Ningbo Academy of Agricultural Sciences, Ningbo, 315040, China

    Huiyan Jia

  7. Inspection and Testing Center of Agricultural and Livestock Products of Tibet, Lhasa, 850000, China

    Sangqiong Lamu

  8. Department of Entomology & Nematology, UC Davis Comprehensive Cancer Center, University of California, Davis, CA, 95616, USA

    Maojun Jin & Bruce D. Hammock

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  1. Yuanshang Wang
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Correspondence to Maojun Jin.

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The original online version of this article was revised: In the original version of this article, the given name and family name of “AM Abd El-Aty” were incorrectly structured.

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Wang, Y., Abd El-Aty, A.M., Chen, G. et al. A competitive immunoassay for detecting triazophos based on fluorescent catalytic hairpin self-assembly. Microchim Acta 189, 114 (2022). https://doi.org/10.1007/s00604-022-05217-5

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