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Optical assay using B-doped core–shell Fe@BC nanozyme for determination of alanine aminotransferase

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Abstract

B-doped core–shell Fe@BC nanozyme was synthesized. The peroxidase (POD) like activity of Fe@BC nanozyme was studied and utilized for detecting the activity of alanine aminotransferase (ALT). In the presence of ALT as well as ALT co-substrates l-alanine and α-ketoglutarate, l-glutamate is generated. The following catalytic oxidation of l-glutamate by glutamate oxidase leads to the generation of H2O2. The POD-like activity of Fe@BC can oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) to oxTMB in the presence of H2O2, generating a blue-colored compound. Through the detection of the amount of H2O2 generated, ALT activity can be determined through measuring the absorbance intensity variation at 450 nm. The limit of detection of the assay is 4 U/L, with a linear range from 10 to 1000 U/L. For human serum samples, the ALT levels determined by our assay are comparable to those determined by the hospital with a correlation coefficient of 0.991, demonstrating the reliability of our assay results.

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References

  1. Ghany MG, Feld JJ, Chang K-M, Chan HLY, Lok ASF, Visvanathan K, Janssen HLA (2020) Serum alanine aminotransferase flares in chronic hepatitis B infection: the good and the bad. Lancet Gastr Hepat 5(4):406–417

    Article  Google Scholar 

  2. Moed S, Zaman MH (2019) A quantitative electrochemical assay for liver injury. Biosensor Bioelectron 131:74–78

    Article  CAS  Google Scholar 

  3. Marcellin P, Kutala BK (2018) Liver diseases: a major, neglected global public health problem requiring urgent actions and large-scale screening. Liver Int 38:2–6

    Article  Google Scholar 

  4. Huang X-J, Choi Y-K, Im H-S, Yarimaga O, Yoon E, Kim H-S (2006) Aspartate aminotransferase (AST/GOT) and alanine aminotransferase (ALT/GPT) detection techniques. Sensors 6(7):756–782

    Article  CAS  Google Scholar 

  5. Carobene A, Braga F, Roraas T, Sandberg S, Bartlett WA (2013) A systematic review of data on biological variation for alanine aminotransferase, aspartate aminotransferase and gamma-glutamyl transferase. Clin Chem Lab Med 51(10):1997–2007

    Article  CAS  Google Scholar 

  6. Pacifico L, Ferraro F, Bonci E, Anania C, Romaggioli S, Chiesa C (2013) Upper limit of normal for alanine aminotransferase: Quo vadis? Clin Chim Acta 422:29–39

    Article  CAS  Google Scholar 

  7. Jamal M, Worsfold O, McCormac T, Dempsey E (2009) A stable and selective electrochemical biosensor for the liver enzyme alanine aminotransferase (ALT). Biosensor Bioelectron 24(9):2926–2930

    Article  CAS  Google Scholar 

  8. Jiang D, Ni D, Rosenkrans ZT, Huang P, Yan X, Cai W (2019) Nanozyme: new horizons for responsive biomedical applications. Chem Soc Rev 48(14):3683–3704

    Article  CAS  Google Scholar 

  9. Wang H, Wan K, Shi X (2019) Recent advances in nanozyme research. Adv Mater 31(45):1805368

    Article  CAS  Google Scholar 

  10. Chen G, Qin Y, Jiao L, Huang J, Wu Y, Hu L, Gu W, Xu D, Zhu C (2021) Nanozyme-activated synergistic amplification for ultrasensitive photoelectrochemical immunoassay. Anal Chem. https://doi.org/10.1021/acs.analchem.1c01217

    Article  PubMed  PubMed Central  Google Scholar 

  11. Fan S, Jiang X, Yang M, Wang X (2021) Sensitive colorimetric assay for the determination of alkaline phosphatase activity utilizing nanozyme based on copper nanoparticle-modified Prussian blue. Anal Bioanal Chem. https://doi.org/10.1007/s00216-021-03347-y

    Article  PubMed  Google Scholar 

  12. Lu W, Chen J, Kong L, Zhu F, Feng Z, Zhan J (2021) Oxygen vacancies modulation Mn3O4 nanozyme with enhanced oxidase-mimicking performance for L-cysteine detection. Sensors Actuat B Chem 333:129560. https://doi.org/10.1016/j.snb.2021.129560

    Article  CAS  Google Scholar 

  13. Kong WS, Guo X, Jing M, QuFengl LuLM (2020) Highly sensitive photoelectrochemical detection of bleomycin based on Au/WS2 nanorod array as signal matrix and Ag/ZnMOF nanozyme as multifunctional amplifier. Biosensor Bioelectron 150:111875–111881

    Article  CAS  Google Scholar 

  14. Liu J, Gao J, Zhang A, Guo Y, Fan S, He Y, Yang K, Wang J, Cui D, Cheng Y (2020) Carbon nanocage-based nanozyme as an endogenous H2O2-activated oxygenerator for real-time bimodal imaging and enhanced phototherapy of esophageal cancer. Nanoscale 12(42):21674–21686

    Article  CAS  Google Scholar 

  15. Mou J, Xu X, Zhang F, Xia J, Wang Z (2020) Promoting nanozyme cascade bioplatform by ZIF-derived N-doped porous carbon nanosheet-based protein/bimetallic nanoparticles for tandem catalysis. ACS Appl Bio Mater 3(1):664–672

    Article  CAS  Google Scholar 

  16. Zhao L, Wu Z, Liu G, Lu H, Gao Y, Liu F, Wang C, Cui J, Lu G (2019) High-activity Mo, S co-doped carbon quantum dot nanozyme-based cascade colorimetric biosensor for sensitive detection of cholesterol. J Mater Chem B 7(44):7042–7051

    Article  CAS  Google Scholar 

  17. Kim MS, Cho S, Joo SH, Lee J, Kwak SK, Kim MI, Lee J (2019) N- and B-codoped graphene: a strong candidate to replace natural peroxidase in sensitive and selective bioassays. ACS Nano 13(4):4312–4321

    Article  CAS  Google Scholar 

  18. Yao Y, Chen H, Qin J, Wu G, Lian C, Zhang J, Wang S (2016) Iron encapsulated in boron and nitrogen codoped carbon nanotubes as synergistic catalysts for Fenton-like reaction. Water Res 101:281–291

    Article  CAS  Google Scholar 

  19. Jiang X, Liu K, Li Q, Liu M, Yang M, Chen X (2021) B-Doped core-shell Fe@BC nanozymes: active site identification and bacterial inhibition. Chem Commun 57(13):1623–1626

    Article  CAS  Google Scholar 

  20. Chen S, Li Z, Huang Z, Jia Q (2021) Investigation of efficient synergistic and protective effects of chitosan on copper nanoclusters: construction of highly active and stable nanozyme for colorimetric and fluorometric dual-signal biosensing. Sensor Actuat B Chem 332:129522

    Article  CAS  Google Scholar 

  21. Wu J, Yang Q, Li Q, Li H, Li F (2021) Two-dimensional MnO2 nanozyme-mediated homogeneous electrochemical detection of organophosphate pesticides without the interference of H2O2 and color. Anal Chem 93(8):4084–4091

    Article  CAS  Google Scholar 

  22. Tran Nguyen Thanh T, Tseng TTC (2016) A micro-platinum wire biosensor for fast and selective detection of alanine aminotransferase. Sensors 16(6):767

    Article  Google Scholar 

  23. Han YD, Song SY, Lee JH, Lee DS, Yoon HC (2011) Multienzyme-modified biosensing surface for the electrochemical analysis of aspartate transaminase and alanine transaminase in human plasma. Anal Bioanal Chem 400(3):797–805

    Article  CAS  Google Scholar 

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Acknowledgements

The authors thank the support of this work by the Hunan Provincial Science and Technology Plan Project, China (No. 2019TP1001) and Innovation-Driven Project of Central South University (2020CX002).

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Authors and Affiliations

  1. Key Laboratory of Transplantation, Chinese Academy of Medical Sciences, Beijing, 100730, China

    Dazhi Tian & Wentao Jiang

  2. Tianjin Key Laboratory for Organ Transplantation, Department of Liver Transplantation, Tianjin First Center Hospital, Tianjin, 300192, China

    Dazhi Tian, Wen Xiang, Hao Wang & Wentao Jiang

  3. Department of Organ Transplantation Center, The Second Xiang-Ya Hospital, Central South University, Changsha, 410011, China

    Ting Li

  4. Hunan Provincial Key Laboratory of Micro & Nano Materials Interface Science, College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, China

    Minghui Yang

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  1. Dazhi Tian
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  2. Wen Xiang
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  3. Hao Wang
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  4. Wentao Jiang
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  5. Ting Li
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  6. Minghui Yang
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Corresponding authors

Correspondence to Wentao Jiang or Ting Li.

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All experiments were in accordance with the guidelines of the National Institute of Health, China, and approved by the Institutional Ethical Committee (IEC) of the Second Xiangya hospital that is attached to Central South University.

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The authors declare no competing interests.

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Tian, D., Xiang, W., Wang, H. et al. Optical assay using B-doped core–shell Fe@BC nanozyme for determination of alanine aminotransferase. Microchim Acta 189, 147 (2022). https://doi.org/10.1007/s00604-021-05056-w

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