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Transition metal dichalcogenides to optimize the performance of peptide-imprinted conductive polymers as electrochemical sensors

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Abstract

Molecularly imprinted polymer (MIP)-based electrochemical sensors for the protein α-synuclein (a marker for Parkinson’s disease) were developed using a peptide epitope from the protein. MIPs doped with various concentrations and species of transition metal dichalcogenides (TMDs) to enhance conductivity were electropolymerized with and without template molecules. The current during the electropolymerization was compared with that associated with the electrochemical response (at 0.24~0.29 V vs. ref. electrode) to target peptide molecules in the finished sensor. We found that this relationship can aid in the rational design of conductive MIPs for the recognition of biomarkers in biological fluids. The sensing range and limit of detection of TMD-doped imprinted poly(AN-co-MSAN)-coated electrodes were 0.001–100 pg/mL and 0.5 fg/mL (SNR = 3), respectively. To show the potential applicability of the MIP electrochemical sensor, cell culture medium from PD patient–specific midbrain organoids generated from induced pluripotent stem cells was analyzed. α-Synuclein levels were found to be significantly reduced in the organoids from PD patients, compared to those generated from age-matched controls. The relative standard deviation and recovery are less than 5% and 95–115%, respectively.

Graphical abstract

Preparation of TMD-doped α-synuclein (SNCA) peptide-imprinted poly(AN-co-MSAN)-coated electrodes.

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Acknowledgements

The authors would like to appreciate the Ministry of Science and Technology of ROC under Contract Nos. MOST 106-2221-E-390-013-MY3, MOST 107-2923-M-390-001-MY3, MOST 108-2923-B-390-001-MY3, and MOST 109-2314-B-390-001-MY3. The JCS lab is supported by the Fonds National de la Recherche (FNR) Luxembourg in the M-era.Net project NanoPD (INTER/MERA/17/11760144). Further work from the JCS lab was supported by the U.S. Army Medical Research Materiel Command endorsed by the U.S. Army through the Parkinson’s Research Program Investigator-Initiated Research Award under Award No. W81XWH-17-PRP-IIRA

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Authors and Affiliations

  1. Department of Materials Science and Engineering, I-Shou University, Kaohsiung, 84001, Taiwan

    Mei-Hwa Lee

  2. Department of Physics and Astronomy, University of New Mexico, Albuquerque, NM, 87131, USA

    James L. Thomas

  3. Department of Chemical and Materials Engineering, National University of Kaohsiung (NUK), 700, Kaohsiung University Rd., Nan-Tzu District, Kaohsiung, 81148, Taiwan

    Zi-Lin Su, Chien-Hsin Yang & Hung-Yin Lin

  4. Department of Electrical Engineering, National University of Kaohsiung, Kaohsiung, 81148, Taiwan

    Wen-Kuan Yeh

  5. Taiwan Semiconductor Research Institute, Hsinchu, 30009, Taiwan

    Wen-Kuan Yeh

  6. Luxembourg Centre for Systems Biomedicine (LCSB), University of Luxembourg, L-4367, Belvaux, Luxembourg

    Anna S. Monzel, Silvia Bolognin & Jens C. Schwamborn

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  1. Mei-Hwa Lee
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Correspondence to Jens C. Schwamborn, Chien-Hsin Yang or Hung-Yin Lin.

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Lee, MH., Thomas, J.L., Su, ZL. et al. Transition metal dichalcogenides to optimize the performance of peptide-imprinted conductive polymers as electrochemical sensors. Microchim Acta 188, 203 (2021). https://doi.org/10.1007/s00604-021-04850-w

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