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An iron oxide nanoworm hybrid on an interdigitated microelectrode silica surface to detect abdominal aortic aneurysms

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Abstract

An abdominal aortic aneurysm (AAA) is abnormal swelling in the abdominal aorta and a prevalent life-threatening disease. This research introduces a new interdigitated microelectrode (IDME)-sensing surface modified by iron oxide nanoworms (IONWs) for detecting the AAA biomarker insulin-like growth factor-1 (IGF1). A sandwich pattern was formulated with the IGF1 aptamer and IGFBP1 (IGF binding protein-1) on the IONW-constructed IDME hybrid to identify IGF1. The surface morphology of the IONWs revealed a uniform distribution of worm-like structures (80–100 nm) as confirmed by FESEM and FETEM analyses. Further, the presence of the major elements, Fe and O, was confirmed by EDX and XPS studies. The crystal planes that appeared in the IONW reflect cubic magnetite. IONW-modified IDME attained a limit of detection for IGF1 of 1 fM (3σ) with an aptamer-IGF1-IGFBP1 sandwich. This sandwich with IGFBP1 enhanced the current level at all concentrations of IGF1 and displayed linearity in the range 1 fM to 100 pM with a determination coefficient of R2 = 0.9373 [y = 3.38221x – 4.79]. Control experiments with complementary aptamer sequences, IGF2 and IGFBP3 did not show notable signal changes, indicating the specific detection of IGF1. This IONW constructed electrode helps to achieve the detection of low amounts of IGF1 and diagnose AAA at the stage prior to rupture.

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Funding

SCBG was supported by a special grant (9001–00596) from Universiti Malaysia Perlis.

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Author notes

  1. Guangjun Yan and Qingchun Li contributed equally to this work.

Authors and Affiliations

  1. Department of Vascular Surgery, China-Japan Union Hospital of Jilin University, Changchun, Jilin, 130031, China

    Guangjun Yan & Xingyu Hong

  2. Department of Gastrointestinal Colorectal and Anal Surgery, China-Japan Union Hospital of Jilin University, Changchun, Jilin, 130031, China

    Qingchun Li & Chang Li

  3. Faculty of Chemical Engineering Technology, Universiti Malaysia Perlis (UniMAP), Arau, Perlis, Malaysia

    Subash C. B. Gopinath

  4. Institute of Nano Electronic Engineering, Universiti Malaysia Perlis (UniMAP), Perlis, Kangar, Malaysia

    Subash C. B. Gopinath

  5. Department of Biological Engineering, College of Engineering, Inha University, Incheon, 402-751, Republic of Korea

    Periasamy Anbu

  6. Department of Cardiology, China-Japan Union Hospital of Jilin University, Jilin Provincial Key Laboratory for Genetic Diagnosis of Cardiovascular Disease, Jilin Provincial Cardiovascular Research Institute, Changchun, Jilin, 130031, China

    Mei Ding

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  1. Guangjun Yan
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Correspondence to Chang Li or Mei Ding.

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Yan, G., Li, Q., Hong, X. et al. An iron oxide nanoworm hybrid on an interdigitated microelectrode silica surface to detect abdominal aortic aneurysms. Microchim Acta 188, 185 (2021). https://doi.org/10.1007/s00604-021-04836-8

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