Abstract
A sensitive ratiometric fluorescence probe based on hybridization chain reaction (HCR) was constructed for sensitive detection of miRNA-21 by using G-triplex and silver nanocluster pairs (AgNC pairs) as an enzyme-free and label-free signal output group. miRNA-21 was used as the primer for the hybridization chain reaction of molecular beacon 1 (MB1) containing the locked G-triplex sequence and molecular beacon 2 (MB2) with intact AgNC pairs at the 5′ and 3′ end activation. The double-stranded product was obtained along with the opening of the G-triplex and the separation of the AgNC pairs. A detection limit of 67 pM and a linear detection range of 0.1–300 nM were obtained for miRNA-21 determination. The proposed strategy enabled the monitoring of miRNA-21 levels in at least three cell lines, indicating that it provided new ideas for detecting miRNA in real samples.
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Acknowledgments
This work was supported by the National Natural Science Foundation of China (No. 21775132), the Natural Science Foundation of Hunan Province (No.2018JJ2388), Hunan 2011 Collaborative Innovation Center of Chemical Engineering & Technology with Environmental Benignity and Effective Resource Utilization, the project of innovation team of the Ministry of Education (IRT_17R90), and “1515” academic leader team program of Hunan Agricultural University.
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Zhao, X., Wang, S., Zou, R. et al. An enzyme-free probe based on G-triplex assisted by silver nanocluster pairs for sensitive detection of microRNA-21. Microchim Acta 188, 55 (2021). https://doi.org/10.1007/s00604-020-04680-2
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DOI: https://doi.org/10.1007/s00604-020-04680-2