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A fluorometric method for aptamer-based simultaneous determination of two kinds of the fusarium mycotoxins zearalenone and fumonisin B1 making use of gold nanorods and upconversion nanoparticles

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Abstract

An aptamer-based assay for the determination of two different kinds of fusarium mycotoxins, i.e., zearalenone (ZEN) and fumonisin B1 (FB1), is presented. Based on the inner filter effect (IFE) strategy, the contents of ZEN and FB1 can be simultaneously quantified. It is making use of 65-nm gold nanorods (AuNRs), 20-nm upconversion nanoparticles (UCNPs), fluorescence dyes, and DNA sequences. In the absence of ZEN and FB1, the UCNPs and AuNRs associate through DNA sequences. Due to IFE effect, weak fluorescence signals are collected. In the presence of ZEN or FB1, UCNPs and AuNRs become unstable and partially separate from each other. This results in the recovery of fluorescence signals. Under 980-nm laser excitation, the logarithmic values of fluorescence signal intensities at 606 nm and 753 nm gradually increase with the concentration of ZEN and FB1 in the ranges 0.05–100 μg L−1 (the coefficient of determination is 0.997) and 0.01–100 ng L−1 (the coefficient of determination is 0.986), respectively. The limits of detection (LOD) of the fabricated assay for ZEN and FB1 are 0.01 μg L−1 and 0.003 ng L−1, respectively. The proposed method has a high selectivity over other competitive mycotoxins, including aflatoxin B1, ochratoxin A, patulin and ochratoxin B. The applicability of the assay was evaluated in the determination of ZEN and FB1 contents in spiked corn samples. The average recoveries ranged from 89.9 to 106.6%. This result confirms the practicality of this method.

Schematic representation of an aptamer-based fluorometric method for simultaneous determination of two kinds of the fusarium mycotoxins zearalenone and fumonisin B1

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Funding

This research was supported by the Nature Science Foundation of China (31901645), Natural Science Foundation of Shandong Province (ZR2019BC088 and ZR2018BC064), Innovation Team cultivated by Jinan City (2018GXRC004) and Special Funds for Taishan Scholars Project.

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Author notes

  1. Deyun He and Zhengzong Wu contributed equally to this work.

Authors and Affiliations

  1. State Key Laboratory of Biobased Material and Green Papermaking, School of Food Science and Engineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan, 250353, China

    Deyun He, Zhengzong Wu & Bo Cui

  2. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China

    Zhengyu Jin

  3. College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, China

    Enbo Xu

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  1. Deyun He
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  2. Zhengzong Wu
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  3. Bo Cui
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  4. Zhengyu Jin
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  5. Enbo Xu
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Correspondence to Zhengzong Wu or Bo Cui.

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He, D., Wu, Z., Cui, B. et al. A fluorometric method for aptamer-based simultaneous determination of two kinds of the fusarium mycotoxins zearalenone and fumonisin B1 making use of gold nanorods and upconversion nanoparticles. Microchim Acta 187, 254 (2020). https://doi.org/10.1007/s00604-020-04236-4

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