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Gold nanoparticles conjugated to bimetallic manganese(II) and iron(II) Prussian Blue analogues for aptamer-based impedimetric determination of the human epidermal growth factor receptor-2 and living MCF-7 cells

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Abstract

An aptamer-based assay is described for the determination of trace levels of the cancer marker human epidermal growth factor receptor-2 (HER2) and living MCF-7 cells. The method is based on the use of a bimetallic MnFe Prussian blue analogue coupled to gold nanoparticles (MnFePBA@AuNP). Compared to pristine MnFe PBA nanocubes, the series of MnFePBA@AuNP exhibits a core-shell spherical nanostructure, and the shell thickness decreases from 99.9 nm down to 49.3 nm on increasing the fraction of AuNPs. The composite was placed on a gold electrode and incubated with the aptamer solution through electrostatic interaction. Then the modified electrode was employed to detect HER2 and MCF-7 cells using [Fe(CN)6]3−/4− as redox probe and displays good responses to both of them. Electrochemical impedance spectroscopy data show that the signal variation between each step during the whole procedure for the HER2 and MCF-7 cells detection can be embodied as the resistance value change between the [Fe(CN)6]3−/4− and electrode surface. The assay has a very low detection limit (0.247 pg∙mL−1) and works in the 0.001–1.0 ng∙mL−1 HER2 concentration range. It was also used to sense HER2 in MCF-7 cells, and this results in an assay that works within the 500–5 × 104 cell∙mL−1 cell concentration range and a 36 cell∙mL−1 detection limit. Furthermore, the aptamer-based assay is selective, acceptably reproducible, stable, and well feasible for the detection of HER2 and living MCF-7 cells in human serum.

Schematic of an electrochemical aptasensor based on the bimetallic MnFe Prussian blue analogue (MnFe PBA) coupling with gold nanoparticles (represented by MnFePBA@AuNPs). It was employed as the aptasensor for human epidermal growth factor receptor-2 (HER2), and living MCF-7 cells.

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Acknowledgements

This work was supported by Programs for the National Natural Science Foundation of China (NSFC: Account Nos. U1604127, U1704256, and 21401168), and Innovative Technology Team of Henan Province (CXTD2014042).

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Authors and Affiliations

  1. Department of Orthopedics, The First Affiliated Hospital of Zhengzhou University, No. 1, Jianshe East Road, Zhengzhou, 450052, People’s Republic of China

    Nan Zhou, Xu Yan & Chunlin Zhang

  2. Henan Provincial Key Laboratory of Surface and Interface Science, Zhengzhou University of Light Industry, No. 136, Science Avenue, Zhengzhou, 450001, People’s Republic of China

    Fangfang Su, Zhenzhen Li, Bin Hu, Linghao He, Minghua Wang & Zhihong Zhang

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  1. Nan Zhou
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  2. Fangfang Su
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  4. Xu Yan
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  6. Bin Hu
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Correspondence to Nan Zhou or Zhihong Zhang.

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Zhou, N., Su, F., Li, Z. et al. Gold nanoparticles conjugated to bimetallic manganese(II) and iron(II) Prussian Blue analogues for aptamer-based impedimetric determination of the human epidermal growth factor receptor-2 and living MCF-7 cells. Microchim Acta 186, 75 (2019). https://doi.org/10.1007/s00604-018-3184-9

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