Skip to main content
SpringerLink
Log in

Voltammetric immunoassay of human IgG based on the release of cadmium(II) from CdS nanocrystals deposited on mesoporous silica nanospheres

  • Original Paper
  • Published:
Microchimica Acta Aims and scope Submit manuscript

Abstract

The authors describe a nanocomposite that was obtained by in-situ deposition of CdS nanocrystals on mesoporous silica nanospheres (MSNs), and its use in an electrochemical immunoassay of human immunoglobulin G (HIgG). The MCN/CdS nanocomposite was covalently modified with the antibodies against HIgG and then employed in a voltammetric immunoassay at antibody-functionalized magnetic beads. Through sandwich immunoreaction, the MCN/CdS nanoprobes are quantitatively captured onto the magnetic beads where numerous Cd(II) ions are released in an acidic solution. The Cd(II) can be detected by anodic stripping voltammetry at a typical working potential of −0.78 V (vs. Ag/AgCl). In combination with the high loading of CdS on MSNs, the use of the stripping voltammetric analysis renders the method high sensitivity. A wide linear range varying from 0.01 to 100 ng mL−1 is obtained for HIgG detection with a lower detection limit at 2.9 pg mL−1. In addition, the preparation of the nanoprobe is inexpensive. The magnetic bead-based assay does not require complex manipulations. Therefore, this method is deemed to possess a wide scope in that it may be applied to other immunoassays.

Schematic presentation of the preparation of the mesoporous silica nanosphere (MSN)/CdS nanocomposite for the electrochemical immunoassay of human IgG at magnetic beads. The high decoration of CdS on MSN and the stripping voltammetric analysis of Cd(II) ions render the method high sensitivity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Chen L, Lv S, Gao Z, Chen C (2017) Voltammetric immunoassay for the carcinoembryonic antigen by using a glassy carbon electrode modified with poly(3,4-ethylenedioxythiophene) doped with tannic acid and grafted with poly (ethylene glycol). Microchim Acta 184:4705–4712

    Article  CAS  Google Scholar 

  2. Gao H, Wang X, Li M, Qi H, Gao Q, Zhang C (2017) Proximity hybridization-regulated electrogenerated chemiluminescence bioassay of α-fetoprotein via target-induced quenching mechanism. Biosens Bioelectron 98:62–67

    Article  CAS  Google Scholar 

  3. Li B, Lai G, Lin B, Yu A, Yang N (2018) Enzyme-induced biomineralization of cupric subcarbonate for ultrasensitive colorimetric immunosensing of carcinoembryonic antigen. Sensors Actuators B Chem 262:789–795

    Article  CAS  Google Scholar 

  4. Wan Y, Su Y, Zhu X, Liu G, Fan C (2013) Development of electrochemical immunosensors towards point of care diagnostics. Biosens Bioelectron 47:1–11

    Article  CAS  Google Scholar 

  5. Shan J, Ma Z (2017) A review on amperometric immunoassays for tumor markers based on the use of hybrid materials consisting of conducting polymers and noble metal nanomaterials. Microchim Acta 184:969–979

    Article  CAS  Google Scholar 

  6. Tang Z, Ma Z (2017) Multiple functional strategies for amplifying sensitivity of amperometric immunoassay for tumor markers: a review. Biosens Bioelectron 98:100–112

    Article  CAS  Google Scholar 

  7. Pei X, Zhang B, Tang J, Liu B, Lai W, Tang D (2013) Sandwich-type immunosensors and immunoassays exploiting nanostructure labels: a review. Anal Chim Acta 758:1–18

    Article  CAS  Google Scholar 

  8. Du D, Wang L, Shao Y, Wang J, Engelhard MH, Lin Y (2011) Functionalized graphene oxide as a nanocarrier in a multienzyme labeling amplification strategy for ultrasensitive electrochemical immunoassay of phosphorylated p53 (S392). Anal Chem 83:746–752

    Article  CAS  Google Scholar 

  9. Xu Q, Wang L, Lei J, Deng S, Ju H (2013) Platinum nanodendrite functionalized graphene nanosheets as a non-enzymatic label for electrochemical immunosensing. J Mater Chem B 1:5347–5352

    Article  CAS  Google Scholar 

  10. Lai G, Zhang H, Tamanna T, Yu A (2014) Ultrasensitive immunoassay based on electrochemical measurement of enzymatically produced polyaniline. Anal Chem 86:1789–1793

    Article  CAS  Google Scholar 

  11. Yang J, Wen W, Zhang X, Wang S (2015) Electrochemical immunosensor for the prostate specific antigen detection based on carbon nanotube and gold nanoparticle amplification strategy. Microchim Acta 182:1855–1861

    Article  CAS  Google Scholar 

  12. Tang D, Yuan R, Chai Y (2008) Ultrasensitive electrochemical immunosensor for clinical immunoassay using thionine-doped magnetic gold nanospheres as labels and horseradish peroxidase as enhancer. Anal Chem 80:1582–1588

    Article  CAS  Google Scholar 

  13. Xu Q, Yan F, Lei J, Leng C, Ju H (2012) Disposable electrochemical immunosensor by using carbon sphere/gold nanoparticle composites as labels for signal amplification. Chem Eur J 18:4994–4998

    Article  CAS  Google Scholar 

  14. Yang M, Li H, Javadi A, Gong S (2010) Multifunctional mesoporous silica nanoparticles as labels for the preparation of ultrasensitive electrochemical immunosensors. Biomaterials 31:3281–3286

    Article  CAS  Google Scholar 

  15. Lu J, Liu S, Ge S, Yan M, Yu J, Hu X (2012) Ultrasensitive electrochemical immunosensor based on au nanoparticles dotted carbon nanotube-graphene composite and functionalized mesoporous materials. Biosens Bioelectron 33:29–35

    Article  Google Scholar 

  16. Cui Z, Cai Y, Wu D, Yu H, Li Y, Mao K, Wang H, Fan H, Wei Q, Du B (2012) An ultrasensitive electrochemical immunosensor for the detection of salbutamol based on Pd@SBA-15 and ionic liquid. Electrochim Acta 69:79–85

    Article  CAS  Google Scholar 

  17. Ma H, Mao K, Li H, Wu D, Zhang Y, Du B, Wei Q (2013) Ultrasensitive multiplexed immunosensors for the simultaneous determination of endocrine disrupting compounds using Pt@SBA-15 as a non-enzymatic label. J Mater Chem B 1:5137–5142

    Article  CAS  Google Scholar 

  18. Medintz IL, Uyeda HT, Goldman ER, Mattoussi H (2005) Quantum dot bioconjugates for imaging, labelling and sensing. Nat Mater 4:435–446

    Article  CAS  Google Scholar 

  19. Tu MC, Chang YT, Kang YT, Chang HY, Chang P, Yew TR (2012) A quantum dot-based optical immunosensor for human serum albumin detection. Biosens Bioelectron 34:286–290

    Article  CAS  Google Scholar 

  20. Li XY, Wang RY, Zhang XL (2011) Electrochemiluminescence immunoassay at a nanoporous gold leaf electrode and using CdTe quantun dots as labels. Microchim Acta 172:285–290

    Article  CAS  Google Scholar 

  21. Zhao WW, Ma ZY, Yan DY, Xu JJ, Chen HY (2012) In situ enzymatic ascorbic acid production as electron donor for CdS quantum dots equipped TiO2 nanotubes: a general and efficient approach for new photoelectrochemical immunoassay. Anal Chem 84:10518–10521

    Article  CAS  Google Scholar 

  22. Liu G, Wang J, Kim J, Jan MR (2004) Electrochemical coding for multiplexed immunoassays of proteins. Anal Chem 76:7126–7130

    Article  CAS  Google Scholar 

  23. Chen L, Chen C, Li R, Li Y, Liu S (2009) CdTe quantum dot functionalized silica nanosphere labels for ultrasensitive detection of biomarker. Chem Commun (19):2670–2672

  24. Shiddiky MJA, Rauf S, Kithva PH, Trau M (2012) Graphene/quantum dot bionanoconjugates as signal amplifiers in stripping voltammetric detection of EpCAM biomarkers. Biosens Bioelectron 35:251–257

    Article  CAS  Google Scholar 

  25. Reiss P, Carrière M, Lincheneau C, Vaure L, Tamang S (2016) Synthesis of semiconductor nanocrystals, focusing on nontoxic and earth-abundant materials. Chem Rev 116:10731–10819

    Article  CAS  Google Scholar 

  26. Han E, Ding L, Jin S, Ju H (2011) Electrochemiluminescent biosensing of carbohydrate-functionalized CdS nanocomposites for in situ label-free analysis of cell surface carbohydrate. Biosens Bioelectron 26:2500–2505

    Article  CAS  Google Scholar 

  27. Cao A, Liu Z, Chu S, Wu M, Ye Z, Cai Z, Chang Y, Wang S, Gong Q, Liu Y (2010) A facile one-step method to produce graphene–CdS quantum dot nanocomposites as promising optoelectronic materials. Adv Mater 22:103–106

    Article  CAS  Google Scholar 

  28. Xiong J, Wang W, Zhou Y, Kong W, Wang Z, Fu Z (2016) Ultra-sensitive chemiluminescent detection of Staphylococcus aureus based on competitive binding of Staphylococcus protein A-modified magnetic beads to immunoglobulin G. Microchim Acta 183:15073–11512

    Article  Google Scholar 

  29. Lai G, Zhang H, Yu A, Ju H (2015) In situ deposition of Prussian blue on mesoporous carbon nanosphere for sensitive electrochemical immunoassay. Biosens Bioelectron 74:660–665

    Article  CAS  Google Scholar 

  30. Peng Y, Xie Y, Luo J, Nie L, Chen Y, Chen L, Du S, Zhang Z (2010) Molecularly imprinted polymer layer-coated silica nanoparticles toward dispersive solid-phase extraction of trace sulfonylurea herbicides from soil and crop samples. Anal Chim Acta 674:190–200

    Article  CAS  Google Scholar 

  31. Wang J, Lu J, Hocevar SB, Farias PAM (2000) Bismuth-coated carbon electrodes for anodic stripping voltammetry. Anal Chem 72:3218–3222

    Article  CAS  Google Scholar 

  32. Wang J (2005) Stripping analysis at bismuth electrodes: a review. Electroanalysis17:1341–1346

  33. Lai G, Zhang H, Yong J, Yu A (2013) In situ deposition of gold nanoparticles on polydopamine functionalized silica nanosphere for ultrasensitive nonenzymatic electrochemical immunoassay. Biosens Bioelectron 47:178–183

    Article  CAS  Google Scholar 

  34. Zhao Y, Zheng Y, Kong R, Xia L, Qu F (2015) Ultrasensitive electrochemical immunosensor based on horseradish peroxidase (HRP)-loaded silica-poly (acrylic acid) brushes for protein biomarker detection. Biosens Bioelectron 75:383–388

    Article  Google Scholar 

  35. Dong S, Tong M, Zhang D, Huang T (2017) The strategy of nitrite and immunoassay human IgG biosensors based on ZnO@ZIF-8 and ionic liquid composite film. Sensors Actuators B Chem 251:650–657

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (21475033), Science and Technology Foundation for Excellent Creative Research Group of Hubei Provincial Department of Education (T201810), and Open Fund of Hubei Key Laboratory of Pollutant Analysis and Reuse Technology (PA20170207).

Author information

Author notes

  1. Peng Guo and Yun Wang contributed equally to this work.

Authors and Affiliations

  1. Hubei Key Laboratory of Pollutant Analysis & Reuse Technology, Department of Chemistry, Hubei Normal University, Huangshi, 435002, People’s Republic of China

    Peng Guo, Yun Wang, Zhichao Chen, Tianqi Jin & Guosong Lai

  2. College of Materials and Environmental Engineering, Hangzhou Dianzi University, Hangzhou, 310018, China

    Li Fu

  3. Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo, 315201, China

    Cheng-Te Lin

Authors
  1. Peng Guo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yun Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Zhichao Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Tianqi Jin
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Li Fu
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Cheng-Te Lin
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Guosong Lai
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Guosong Lai.

Ethics declarations

The authors declare that they have no competing interests.

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic supplementary material

ESM 1

(DOC 320 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Guo, P., Wang, Y., Chen, Z. et al. Voltammetric immunoassay of human IgG based on the release of cadmium(II) from CdS nanocrystals deposited on mesoporous silica nanospheres. Microchim Acta 186, 15 (2019). https://doi.org/10.1007/s00604-018-3142-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s00604-018-3142-6

Keywords

Search

Navigation