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A NIR-responsive up-conversion nanoparticle probe of the NaYF4:Er,Yb type and coated with a molecularly imprinted polymer for fluorometric determination of enrofloxacin

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Abstract

The paper describes the preparation of a fluorescent probe based on the use of up-conversion particles (UCPs) of the type NaYF4: Er, Yb and shelled with a molecularly imprinted polymer (MIP) that is specific for the antibiotic enrofloxacin. The upconverted green luminescence emitted by the UCPs upon photoexcitation at 980 nm was used to locally generate a thin MIP shell around the UCPs via photopolymerization. Enrofloxacin is used as the template, and ethylene glycol dimethacrylate (EGDMA) as the cross-linking agent. It is found that enrofloxacin is well captured by the MIP and then quenches the fluorescence of the UCPs. Fluorescence decreases linearly in the 63 ng·L−1 to 60 μg·L−1 enrofloxacin concentration range, with a 8 ng·L−1 limit of detection. The method was applied to the determination of enrofloxacin in spiked fish samples. Recoveries ranged from 83.7 to 87.7%, with relative standard deviations between 2.0 and 5.5% (for n = 3).

Schematic of an NIR-responsive up-conversion nanoparticle probe of the NaYF4:Er,Yb type and coated with a MIP for fluorometric determination of enrofloxacin. The upconverted luminescence emitted by the UCPs was used to locally generate a thin MIP shell around the UCPs via photopolymerization.

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Acknowledgments

This work was financially supported by the National Natural Science of China (Grant No. 31201370) and the Graduate Innovation Foundation of Bohai University (071502224-49).

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Authors and Affiliations

  1. College of Food Science & Project Engineering, Bohai University, Food Safety Key Lab of Liaoning Province, National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, Jinzhou, Liaoning, 121013, China

    Yiwei Tang, Min Li, Xue Gao, Xiuying Liu, Jinwen Gao, Tao Ma & Jianrong Li

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  1. Yiwei Tang
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  2. Min Li
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  3. Xue Gao
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  4. Xiuying Liu
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  6. Tao Ma
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Correspondence to Yiwei Tang, Xiuying Liu or Jianrong Li.

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Tang, Y., Li, M., Gao, X. et al. A NIR-responsive up-conversion nanoparticle probe of the NaYF4:Er,Yb type and coated with a molecularly imprinted polymer for fluorometric determination of enrofloxacin. Microchim Acta 184, 3469–3475 (2017). https://doi.org/10.1007/s00604-017-2387-9

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  • DOI: https://doi.org/10.1007/s00604-017-2387-9

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