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Reagentless and label-free voltammetric immunosensor for carcinoembryonic antigen based on polyaniline nanowires grown on porous conducting polymer composite

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Abstract

A reagentless and label-free voltammetric modified electrode was developed for the carcinoembryonic antigen (CEA), an important biomarker for colorectal adenocarcinoma. It is based on the use of a reticular hybrid composite consisting of polyaniline (PANI) nanowires grown on the conducting polymer poly(3,4-ethylenedioxythiophene) (PEDOT) that was doped with an ionic liquid (IL). The composite features excellent electrical conductivity and a porous structure, a high specific surface and inherent redox activity. It was placed on a glassy carbon electrode (GCE) and antibody against CEA was immobilized on its surface. The redox current of PANI, measured typically at 0.16 V (vs. Ag/AgCl) serves as the analytical information. This electrode displays linear range that extends from 0.001 to 10 ng mL−1, with a detection limit as low as 0.7 pg mL−1. It also possesses excellent temporal stability and selectivity.

A sensitive, label-free and reagentless modified electrode for CEA was developed based on the combination of two different conducting polymers, polyaniline nanowires and porous PEDOT/IL nanocomposite.

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Acknowledgements

This research was supported by the National Natural Science Foundation of China (21275087, 21422504), the Natural Science Foundation of Shandong Province of China (JQ201406 and ZR2016BM05), and the Taishan Scholar Program of Shandong Province of China.

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Authors and Affiliations

  1. Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, China

    Xiaotian Sun, Ni Hui & Xiliang Luo

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  1. Xiaotian Sun
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  2. Ni Hui
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Correspondence to Xiliang Luo.

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Sun, X., Hui, N. & Luo, X. Reagentless and label-free voltammetric immunosensor for carcinoembryonic antigen based on polyaniline nanowires grown on porous conducting polymer composite. Microchim Acta 184, 889–896 (2017). https://doi.org/10.1007/s00604-016-2068-0

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  • DOI: https://doi.org/10.1007/s00604-016-2068-0

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