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Potentiometric competitive immunoassay for determination of aflatoxin B1 in food by using antibody-labeled gold nanoparticles

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Abstract

The authors report on a competitive potentiometric immunoassay for aflatoxin B1 (AFB1) in food that displays distinctly improved sensitivity. Gold nanoparticles (AuNPs; 16 nm i. d.) were functionalized with polyclonal anti-AFB1 antibody (pAb), whilst the sensor electrode was prepared by immobilizing AFB1-bovine serum albumin conjugate (AFB1-BSA) on a glassy carbon electrode. Upon addition of target AFB1, competitive immunobinding occurs between the analyte and AFB1-BSA for the labeled pAb on the AuNPs. The change in the surface charge as a result of the antigen-antibody reaction causes a shift in the electrical potential. With increasing concentrations of analyte (AFB1), the quantity of pAb-AuNP captured by the electrode decreases. The shift in the output potential is linearly proportional to the logarithm of AFB1 concentration in the 0.1 to 5.0 μg · kg−1 range, with a detection limit (LOD) of 87 ng · kg−1 (87 ppt). An intermediate precision of 10.9 % was accomplished in batch-to-batch identification. The selectivity over AFB2 with similar chemical structure is acceptable. The method accuracy was evaluated by analyzing naturally contaminated and spiked peanut samples, giving consistent results (with RSD values of <12 %) between this immunoassay and the commercial ELISA.

A potentiometric immunosensor was designed for detection of AFB1 by using nanogold-labeled antibodies as the signal-amplification tags.

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Acknowledgments

Support by the National Natural Science Foundation of China (41176079 & 21475025), the Research Fund for the Doctoral Program of Hubei University for Nationalities (MY2014B014), the National Science Foundation of Fujian Province (2014J07001), and the Program for Changjiang Scholars and Innovative Research Team in University (IRT1116) is gratefully acknowledged.

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Authors and Affiliations

  1. School of Chemistry and Environmental Engineering, Hubei University for Nationalities, Enshi, 445000, People’s Republic of China

    Qunfang Li

  2. Institute of Environmental and Analytical Science, School of Chemistry and Chemical Engineering, Henan University, Kaifeng, Henan, 475004, People’s Republic of China

    Minghua Lu

  3. MOE Key Laboratory of Analysis and Detection for Food Safety, Institute of Nanomedicine and Nanobiosensing, Department of Chemistry, Fuzhou University, Fuzhou, 350108, People’s Republic of China

    Qunfang Li, Shuzhen Lv, Zhenzhen Lin & Dianping Tang

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  1. Qunfang Li
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Correspondence to Minghua Lu.

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Li, Q., Lv, S., Lu, M. et al. Potentiometric competitive immunoassay for determination of aflatoxin B1 in food by using antibody-labeled gold nanoparticles. Microchim Acta 183, 2815–2822 (2016). https://doi.org/10.1007/s00604-016-1929-x

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  • DOI: https://doi.org/10.1007/s00604-016-1929-x

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