Skip to main content
SpringerLink
Log in

Direct electrochemistry of glucose oxidase and a biosensor for glucose based on a glass carbon electrode modified with MoS2 nanosheets decorated with gold nanoparticles

  • Original Paper
  • Published:
Microchimica Acta Aims and scope Submit manuscript

Abstract

An electrochemical glucose biosensor was developed by immobilizing glucose oxidase (GOx) on a glass carbon electrode that was modified with molybdenum disulfide (MoS2) nanosheets that were decorated with gold nanoparticles (AuNPs). The electrochemical performance of the modified electrode was investigated by cyclic voltammetry, and it is found that use of the AuNPs-decorated MoS2 nanocomposite accelerates the electron transfer from electrode to the immobilized enzyme. This enables the direct electrochemistry of GOx without any electron mediator. The synergistic effect the MoS2 nanosheets and the AuNPs result in excellent electrocatalytic activity. Glucose can be detected in the concentration range from 10 to 300 μM, and down to levels as low as 2.8 μM. The biosensor also displays good reproducibility and long-term stability, suggesting that it represents a promising tool for biological assays.

A MoS2-based glucose sensor has been prepared by gold nanoparticles-decorated MoS2 nanocomposite, which exhibited excellent electrocatalytic activity, reproducibility and long-term stability. It was applied to determine glucose concentration in human serum, suggest the sensor maybe promising for practical application.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Wang J (2001) Glucose biosensors: 40 years of advances and challenges. Electroanalysis 13:983–988. doi:10.1002/1521-4109(200108)13:12<983::AID-ELAN983>3.0.CO;2-#

    Article  CAS  Google Scholar 

  2. Wang J (2008) Electrochemical glucose biosensors. Chem Rev 108:814–825. doi:10.1021/cr068123a

    Article  CAS  Google Scholar 

  3. Shan CS, Yang HF, Song JF, Han DX, Ivaska A, Niu L (2009) Direct electrochemistry of glucose oxidase and biosensing for glucose based on graphene. Anal Chem 81:2378–2382. doi:10.1021/ac802193c

    Article  CAS  Google Scholar 

  4. Zhang SX, Wang N, Yu HJ, Niu YM, Sun CQ (2005) Covalent attachment of glucose oxidase to an Au electrode modified with gold nanoparticles for use as glucose biosensor. Bioelectrochem 67:15–22. doi:10.1016/j.bioelechem.2004.12.002

    Article  CAS  Google Scholar 

  5. Hrapovic S, Liu YL, Keith B, Luong JHT (2004) Electrochemical biosensing platforms using platinum nanoparticles and carbon nanotubes. Anal Chem 76:1083–1088. doi:10.1021/ac035143t

    Article  CAS  Google Scholar 

  6. Liu Y, Wang MK, Zhao F, Xu ZA, Dong SJ (2005) The direct electron transfer of glucose oxidase and glucose biosensor based on carbon nanotubes/chitosan matrix. Biosens Bioelectron 21:984–988. doi:10.1016/j.bios.2005.03.003

    Article  CAS  Google Scholar 

  7. Tang YF, Allen BL, Kauffman DR, Star A (2009) Electrocatalytic activity of nitrogen-doped carbon nanotube cups. J Am Chem Soc 131:13200–13201. doi:10.1021/ja904595t

    Article  CAS  Google Scholar 

  8. Zhou M, Zhai YM, Dong SJ (2009) Electrochemical sensing and biosensing platform based on chemically reduced graphene oxide. Anal Chem 81:5603–5613. doi:10.1021/ac900136z

    Article  CAS  Google Scholar 

  9. Chen D, Tang LH, Li JH (2010) Graphene-based materials in electrochemistry. Chem Soc Rev 39:3157–3180. doi:10.1039/B923596E

    Article  CAS  Google Scholar 

  10. Su S, He Y, Song SP, Li D, Wang LH, Fan CH, Lee ST (2010) A silicon nanowire-based electrochemical glucose biosensor with high electrocatalytic activity and sensitivity. Nanoscale 2:1704–1707. doi:10.1039/C0NR00314J

    Article  CAS  Google Scholar 

  11. Shao MW, Shan YY, Wong NB, Lee ST (2005) Silicon nanowire sensors for bioanalytical applications: glucose and hydrogen peroxide detection. Adv Funct Mater 15:1478–1482. doi:10.1002/adfm.200500080

    Article  CAS  Google Scholar 

  12. Wang JP, Thomas DF, Chen AC (2008) Nonenzymatic electrochemical glucose sensor based on nanoporous PtPb networks. Anal Chem 80:997–1004. doi:10.1021/ac701790z

    Article  CAS  Google Scholar 

  13. Zhao M, Wu XM, Cai CX (2009) Polyaniline nanofibers: synthesis, characterization, and application to direct electron transfer of glucose oxidase. J Phys Chem C 113:4987–4996. doi:10.1021/jp807621y

    Article  CAS  Google Scholar 

  14. Yu T, Lim B, Xia YN (2010) Aqueous-phase synthesis of single-crystal ceria nanosheets. Angew Chem Int Ed 49:4484–4487. doi:10.1002/anie.201001521

    Article  CAS  Google Scholar 

  15. Advanced Functional MaterialsBrugger T, Günther S, Wang B, Dil JH, Bocquet ML, Osterwalder J, Wintterlin J, Greber T (2009) Comparison of electronic structure and template function of single-layer graphene and a hexagonal boron nitride nanomesh on Ru (0001). Phys Rev B 79:045407. doi:10.1103/PhysRevB.79.045407

    Article  Google Scholar 

  16. Wang QH, Kalantar-Zadeh K, Kis A, Coleman JN, Strano MS (2012) Electronics and optoelectronics of two-dimensional transition metal dichalcogenides. Nat Nanotechnol 7:699–712. doi:10.1038/nnano.2012.193

    Article  CAS  Google Scholar 

  17. Ramakrishna Matte HSS, Gomathi A, Manna AK, Late DJ, Datta R, Pati SK, Rao CNR (2012) MoS2 And WS2 analogues of graphene. Angew Chem Int Ed 122:4153–4156. doi:10.1002/ange.201000009

    Article  Google Scholar 

  18. Kibsgaard J, Chen ZB, Reinecke BN, Jaramillo TF (2012) Engineering the surface structure of MoS2 to preferentially expose active edge sites for electrocatalysis. Nat Mater 11:963–969. doi:10.1038/nmat3439

    Article  CAS  Google Scholar 

  19. Zong X, Wu GP, Yan HJ, Ma GJ, Shi JY, Wen FY, Wang L, Li C (2010) Photocatalytic H2 evolution on MoS2/CdS catalysts under visible light irradiation. J Phys Chem C 114:1963–1968. doi:10.1021/jp904350e

    Article  CAS  Google Scholar 

  20. Hwang H, Kim H, Cho J (2011) MoS2 nanoplates consisting of disordered graphene-like layers for high rate lithium battery anode materials. Nano Lett 11:4826–4830. doi:10.1021/nl202675f

    Article  CAS  Google Scholar 

  21. Chang K, Chen W (2011) In situ synthesis of MoS2/graphene nanosheet composites with extraordinarily high electrochemical performance for lithium ion batteries. Chem Commun 47:4252–4254. doi:10.1039/C1CC10631G

    Article  CAS  Google Scholar 

  22. Radisavljevic B, Radenovic A, Brivio J, Giacometti V, Kis A (2011) Single-layer MoS2 transistors. Nat Nanotechnol 6:147–150. doi:10.1038/nnano.2010.279

    Article  CAS  Google Scholar 

  23. Splendiani A, Sun L, Zhang Y, Li T, Kim J, Chim CY, Galli G, Wang F (2010) Emerging photoluminescence in monolayer MoS2. Nano Lett 10:1271–1275. doi:10.1021/nl903868w

    Article  CAS  Google Scholar 

  24. Yoon Y, Ganapathi K, Salahuddin S (2011) How good can monolayer MoS2 transistors be? Nano Lett 11:3768–3773. doi:10.1021/nl2018178

    Article  CAS  Google Scholar 

  25. Li H, Yin ZY, He QY, Huang X, Lu G, Fam DWH, Tok AIY, Zhang Q, Zhang H (2012) Fabrication of single- and multilayer MoS2 film-based field-effect transistors for sensing NO at room temperature. Small 8:63–67. doi:10.1002/smll.201101016

    Article  CAS  Google Scholar 

  26. Wu SX, Zeng ZY, He QY, Wang ZY, Wang SJ, Du YP, Yin ZY, Sun XP, Chen W, Zhang H (2012) Electrochemically reduced single-layer MoS2 nanosheets: characterization, properties, and sensing applications. Small 8:2264–2270. doi:10.1002/smll.201200044

    Article  CAS  Google Scholar 

  27. Huang X, Zeng Z, Bao S, Wang M, Qi X, Fan Z, Zhang H (2013) Solution-phase epitaxial growth of noble metal nanostructures on dispersible single-layer molybdenum disulfide nanosheets. Nat Commun 4:1444. doi:10.1038/ncomms2472

    Article  Google Scholar 

  28. Joensen P, Frindt RF, Morrison SR (1986) Single-layer MoS2. Mater Res Bull 21:457–461. doi:10.1016/0025-5408(86)90011-5

    Article  CAS  Google Scholar 

  29. Wang K, Liu Q, Guan QM, Wu J, Li HN, Yan JJ (2011) Enhanced direct electrochemistry of glucose oxidase and biosensing for glucose via synergy effect of graphene and CdS nanocrystals. Biosens Bioelectron 26:2252–2257. doi:10.1016/j.bios.2010.09.043

    Article  CAS  Google Scholar 

  30. Chen Y, Li Y, Sun D, Tian DB, Zhang JR, Zhu JJ (2011) Fabrication of gold nanoparticles on bilayer graphene for glucose electrochemical biosensing. J Mater Chem 21:7604–7611. doi:10.1039/C1JM10293A

    Article  CAS  Google Scholar 

  31. Zhao S, Zhang K, Bai Y, Yang WW, Sun CQ (2006) Glucose oxidase/colloidal gold nanoparticles immobilized in Nafion film on glassy carbon electrode: direct electron transfer and electrocatalysis. Bioelectrochem 69:158–163. doi:10.1016/j.bioelechem.2006.01.001

    Article  CAS  Google Scholar 

  32. Shan CS, Yang HF, Han DX, Zhang QX, Ivaska A, Niu L (2010) Graphene/AuNPs/chitosan nanocomposites film for glucose biosensing. Biosens Bioelectron 25:1070–1074. doi:10.1016/j.bios.2009.09.024

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was financially supported by the National Basic Research Program of China (2012CB933301, 2009CB930600), the National Natural Science Foundation of China (21305070, 21204038, 81273409), the Ministry of Education of China (IRT1148, 20123223110007, 20133223120013), the Natural Science Foundation of Jiangsu Province (BK20130861), the Scientific Research Foundation of Nanjing University of Posts and Telecommunications (NY212033), the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), the Open Research Fund of State Key Laboratory of Bioelectronics, Southeast University.

Author information

Authors and Affiliations

  1. Key Laboratory for Organic Electronics & Information Displays (KLOEID), Institute of Advanced Materials (IAM), and School of Materials Science and Engineering, Nanjing University of Posts & Telecommunications, 9 Wenyuan Road, Nanjing, 210046, China

    Shao Su, Haofan Sun, Fei Xu, Lihui Yuwen, Chunhai Fan & Lianhui Wang

  2. Division of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, 201800, People’s Republic of China

    Chunhai Fan

Authors
  1. Shao Su
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Haofan Sun
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Fei Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Lihui Yuwen
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Chunhai Fan
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Lianhui Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Chunhai Fan or Lianhui Wang.

Electronic supplementary material

Below is the link to the electronic supplementary material.

ESM 1

(DOC 293 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Su, S., Sun, H., Xu, F. et al. Direct electrochemistry of glucose oxidase and a biosensor for glucose based on a glass carbon electrode modified with MoS2 nanosheets decorated with gold nanoparticles. Microchim Acta 181, 1497–1503 (2014). https://doi.org/10.1007/s00604-014-1178-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00604-014-1178-9

Keywords

Search

Navigation