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Electrochemical tyrosine sensor based on a glassy carbon electrode modified with a nanohybrid made from graphene oxide and multiwalled carbon nanotubes

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Abstract

We report on a glassy carbon electrode that was modified with a composite made from graphene oxide (GO) and multiwalled carbon nanotubes (MWCNT) that enables highly sensitive determination of L-tyrosine. The sensor was characterized by transmission electron microscopy and electrochemical impedance spectroscopy, and its electrochemical properties by cyclic voltammetry, chronocoulometry and differential pulse voltammetry. The GO/MWCNT hybrid exhibits strong catalytic activity toward the oxidation of L-tyrosine, with a well defined oxidation peak at 761 mV. The respective current serves as the analytical information and is proportional to the L-tyrosine concentration in two ranges of different slope (0.05 to 1.0 μM and 1.0 to 650.0 μM), with limits of detection and quantification as low as 4.4 nM and 14.7 nM, respectively. The method was successfully applied to the analysis of L-tyrosine in human body fluids. The excellent reproducibility, stability, sensitivity and selectivity are believed to be due to the combination of the electrocatalytic properties of both GO and MWCNT. They are making this hybrid electrode a potentially useful electrochemical sensing platform for bioanalysis.

A new L-tyrosine electrochemical sensor was fabricated based on graphene oxide and multiwalled carbon nanotube. The prepared sensor exhibits excellent electro-catalysis to the oxidation of L-tyrosine, and can improve determination sensitivity and decrease detection limit. This sensor was successfully applied to detect L-tyrosine in human fluids with satisfactory results.

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Acknowledgments

This work was kindly supported by the Scientific Research Project of Education Department of Hunan Province (nos. 12C0536, 10K010), Open Fund Project of Key Laboratory (nos. 11K02) and the Youth Backbone Teacher Training Program of Hengyang Normal University (2010).

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Authors and Affiliations

  1. Key Laboratory of Functional Organometallic Materials of Hunan Province College, Department of Chemistry and Material Science, Hengyang Normal University, Hunan, Hengyang, 421008, People’s Republic of China

    Junhua Li, Daizhi Kuang, Yonglan Feng, Fuxing Zhang, Zhifeng Xu, Mengqin Liu & Deping Wang

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  1. Junhua Li
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  2. Daizhi Kuang
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  3. Yonglan Feng
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  4. Fuxing Zhang
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  5. Zhifeng Xu
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  6. Mengqin Liu
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  7. Deping Wang
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Correspondence to Junhua Li.

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Li, J., Kuang, D., Feng, Y. et al. Electrochemical tyrosine sensor based on a glassy carbon electrode modified with a nanohybrid made from graphene oxide and multiwalled carbon nanotubes. Microchim Acta 180, 49–58 (2013). https://doi.org/10.1007/s00604-012-0905-3

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  • DOI: https://doi.org/10.1007/s00604-012-0905-3

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