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Electrochemiluminescence immunoassay at a nanoporous gold leaf electrode and using CdTe quantun dots as labels

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Abstract

An electrochemiluminescence-based immunoassay using quantum dots (QDs) as labels for the carcinoembryonic antigen (CEA) was developed using an electrode modified with leafs of nanoporous gold. CEA was initially immobilized on the electrode via a sandwich immunoreaction, and then CdTe quantum dots capped with thioglycolic acid were used to label the second antibody. The intensity of the ECL of the QDs reflects the quantity of CEA immobilized on the electrode. Thus, in the presence of dithiopersulfate as the coreactant, the ECL serves as the signal for the determination of CEA. The intensity of the electroluminescence (ECL) of the electrode was about 5.5-fold higher than that obtained with a bare gold electrode. The relation between ECL intensity and CEA concentration is linear in the range from 0.05 to 200 ng.mL-1, and the detection limit is 0.01 ng.mL-1. The method has the advantages of high sensitivity, good reproducibility and long-term stability, and paves a new avenue for applying quantum dots in ECL-based bioassays.

Electrochemiluminescence Immunoassay Based on CdTe Quantun Dots as labels at Nanoporous Gold Leaf electrode

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Acknowledgments

This project was supported by the National Natural Science Foundation of China (Grant No. 20975061), the National Basic Research Program of China (Grant No. 2007CB936602), the Natural Science Foundation of Shandong Province in China (Grant No. Y2008B20). We are grateful to Professor Yi Ding’s group (School of Chemistry and Chemical Engineering, Shandong University) for providing the NPGL.

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  1. School of Chemistry and Chemical Engineering, Shandong University, Jinan, 250100, China

    Xiaoyu Li, Rongyue Wang & Xiaoli Zhang

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  1. Xiaoyu Li
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  2. Rongyue Wang
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  3. Xiaoli Zhang
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Correspondence to Xiaoli Zhang.

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Li, X., Wang, R. & Zhang, X. Electrochemiluminescence immunoassay at a nanoporous gold leaf electrode and using CdTe quantun dots as labels. Microchim Acta 172, 285–290 (2011). https://doi.org/10.1007/s00604-010-0487-x

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  • DOI: https://doi.org/10.1007/s00604-010-0487-x

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