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Evaluation of DNA damage and antioxidant capacity of sericin by a DNA electrochemical biosensor based on dendrimer-encapsulated Au-Pd/chitosan composite

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Abstract

Au-Pd bimetallic nanoparticles were prepared in the presence of an amine-terminated dendrimer of a fourth generation poly(amidoamine) type. A biosensor was fabricated by immobilizing dsDNA on a thin layer of a dendrimer-encapsulated bimetallic nanoparticles (Au-Pd) in a chitosan composite on a glassy carbon electrode. The biosensor was evaluated by square wave voltammetry for the determination of the oxidative damage of immobilized DNA and the antioxidant capacity of sericin. The biosensor is shown to be suitable for the rapid detection of DNA damage and the assessment of the antioxidant capacity of sericin.

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Acknowledgment

This work was supported by the National Natural Science Foundation of China (No. 20775044), and the Natural Science Foundation of Shandong Province (No. Y2006B20).

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Authors and Affiliations

  1. College of Chemistry and Material Science, Shandong Agricultural University, Taian, 271018, China

    Ping Qian, Shiyun Ai, Huanshun Yin & Jinhuan Li

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  1. Ping Qian
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  2. Shiyun Ai
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  3. Huanshun Yin
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  4. Jinhuan Li
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Correspondence to Shiyun Ai.

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Qian, P., Ai, S., Yin, H. et al. Evaluation of DNA damage and antioxidant capacity of sericin by a DNA electrochemical biosensor based on dendrimer-encapsulated Au-Pd/chitosan composite. Microchim Acta 168, 347–354 (2010). https://doi.org/10.1007/s00604-009-0280-x

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