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Differences in sensitivity to tumor-specific CTLs between primary and metastatic esophageal cancer cell lines derived from the same patient

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Abstract

Purpose

MHC antigens and adhesion molecules, such as the intracellular adhesion molecule (ICAM-I), play an important role in cellular immune response. We examined the expression patterns of these molecules in both primary and metastatic esophageal carcinoma cells from the same patient and evaluated the cellular immune responses against these cells.

Materials and methods

In the esophageal cancer patient (H122), tumor cell lines were established from primary and subcutaneous metastatic lesions. We compared the expression of cell surface molecules on the metastatic tumor cell line (H122SC) with that on the primary tumor cell line (H122ESO) using flow cytometry. Moreover, we analyzed the differences in cellular immune responses against these cell lines, which expressed similar levels of the Tara antigen, using the Tara antigen-specific CTL clone.

Results

H122SC ICAM-1 expression was significantly lower in H122ESO, and the Tara antigen-specific CTL clone produced lower levels of TNF in response to H122SC than H122ESO. ICAM-1 transfection into the H122SC rendered these cells as sensitive to the CTL clone as the H122ESO.

Conclusion

The metastatic tumor cells displayed lower regulated ICAM-1 expression levels and were less sensitive to specific CTLs. ICAM-1 downregulation may be one mechanism by which tumor cells escape immunologic surveillance.

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Abbreviations

ICAM-1:

Intracellular adhesion molecule-1

LFA-3:

Leukocyte function antigen 3

Fas-L:

Fas ligand

sICAM-1:

Serum soluble intracellular adhesion molecule-1

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Acknowledgments

This study was funded in part by a grant-in-aid from the Ministry of Health, Labour and Welfare, Japan and by a grant-in-aid from the Ministry of Education, Culture, Sports, Science and Technology, Japan. We thank Mrs. Kahoru Noda, Ayako Yamasaki and Miki Shimada for their technical expert help and appreciate the assistance of Mr. Brian Quinn for critical comments on the manuscript.

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Authors and Affiliations

  1. Second Department of Surgery, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan

    Yoshinobu Ichiki, Takeshi Hanagiri, Mitsuhiro Takenoyama, Tetsuro Baba, Yoshika Nagata, Makiko Mizukami, Tetsuya So, Masakazu Sugaya, Manabu Yasuda, Hidetaka Uramoro & Kosei Yasumoto

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  1. Yoshinobu Ichiki
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  2. Takeshi Hanagiri
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  3. Mitsuhiro Takenoyama
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  11. Kosei Yasumoto
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Correspondence to Yoshinobu Ichiki.

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Ichiki, Y., Hanagiri, T., Takenoyama, M. et al. Differences in sensitivity to tumor-specific CTLs between primary and metastatic esophageal cancer cell lines derived from the same patient. Surg Today 42, 272–279 (2012). https://doi.org/10.1007/s00595-011-0083-7

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  • DOI: https://doi.org/10.1007/s00595-011-0083-7

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