Abstract
Purpose
We investigated the effects of bombesin on disseminated candidiasis, and compared the effectiveness of bombesin with Saccharomyces boulardii against Candida albicans translocation from the gastrointestinal tract in immunosuppressed rats.
Methods
Sixty rats were divided into five groups of 12. Group 1 was given only a laboratory pellet diet and water during the experiments; the other four groups were orally inoculated with C. albicans; and groups 3, 4, and 5 were also given prednisolone intraperitoneally. The treatment groups consisted of group 4, given S. boulardii orally, and group 5, given bombesin subcutaneously. The rats were killed after 10 days, and the large bowel, liver, spleen, and kidneys were removed for microbiological and histopathological examination. Blood samples were taken to measure tumor necrosis factor-α (TNF-α) and interleukin-1 β (IL-1β) levels, and the results were compared.
Results
The number of translocated C. albicans colonies from the gastrointestinal tract and the serum TNF-α and IL-β levels were significantly lower in groups 4 and 5 than in group 3 (P < 0.05). Histological analysis revealed that the bombesin-treated group (group 5) had significantly less mucosal ulceration and submucosal inflammation in the large bowel, less inflammation and necrosis in the liver, and less inflammation of the Bowman capsules in the kidney than the S. boulardii-treated group (group 4) (P < 0.05).
Conclusions
These findings show that both S. boulardii and bombesin inhibit the translocation of C. albicans from the gastrointestinal tract, although mucosal ulceration, submucosal inflammation in the large bowel, and dissemination in the liver and kidneys were significantly less severe in the bombesin-treated immunosuppressed rats.
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References
C Aybay T Imir (1996) ArticleTitleTumor necrosis factor (TNF) induction from monocyte/macrophages by Candida species Immunobiology 196 363–74 Occurrence Handle9061377
RD Berg (1992) Translocation and the indigenous gut flora R Fuller (Eds) Probiotics. The scientific basis Chapman and Hall London 55–85
JF Forstner GG Forstner (1994) Gastrointestinal mucus LR Johnson (Eds) Physiology of the gastrointestinal tract EditionNumber3rd ed. Raven Press New York 1255–84
LJ Douglas (1988) ArticleTitleCandida proteinases and candidosis Crit Rev Biotechnol 8 121–9 Occurrence Handle3067860
L Romani P Puccetti F Bisconi (1996) ArticleTitleBiological role of Th cell subsets in candidiasis Chem Immunol 63 115–37 Occurrence Handle8934834
MW Marino A Dunn D Grail M Inglese Y Noguchi E Richards et al. (1997) ArticleTitleCharacterization of tumor necrosis factor-deficient mice Proc Natl Acad Sci U S A 94 8093–8 Occurrence Handle10.1073/pnas.94.15.8093 Occurrence Handle9223320
RB Sartor (1994) ArticleTitleCytokines in intestinal inflammation: pathophysiological and clinical considerations Gastroenterology 106 533–9 Occurrence Handle8299918
VL Cohan AL Scott CA Dinarello RA Prendergast (1991) ArticleTitleInterleukin-1 is a mucus secretagogue Cell Immunol 136 425–34 Occurrence Handle10.1016/0008-8749(91)90364-H Occurrence Handle1873825
CM Surawicz GW Elmer P Speelman LV McFarland J Chinn G van Belle (1989) ArticleTitlePrevention of antibiotic-associated diarrhea by Sacharomyces boulardii: a prospective study Gastroenterology 96 981–8 Occurrence Handle2494098
R Berg P Bernasconi D Fowler M Gautreaux (1993) ArticleTitleInhibition of Candida albicans translocation from the gastrointestinal tract of mice by oral administration of Saccharamyces boulardii J Infect Dis 168 1314–8 Occurrence Handle8228371
A Anastasi V Erspamer M Bucci (1972) ArticleTitleIsolation and amino acid sequences of alytesin and bombesin, two analogous active tetradecapeptides from the skin of European discoglossid frogs Arch Biochem Biophys 148 443–6 Occurrence Handle10.1016/0003-9861(72)90162-2 Occurrence Handle4537042
TJ Macdonald G Nilsson M Vagne M Ghatei SR Bloom V Mutt (1978) ArticleTitleA gastrin-releasing peptide from the porcine non-gastric tissue Gut 1978 767–74
E Rozengurt (1996) Neuropeptide growth factors: signaling pathways and role in cancer LL Pusztai CE Lewis E Yap (Eds) Cell proliferation in cancer: regulatory mechanism of neoplastic cell growth Oxford University Press Oxford 247–59
M Costa JB Furness C Yanaihara TW Moody (1984) ArticleTitleDistribution and projection of neurons with immunoreactivity to both gastrin-releasing peptide and bombesin in guinea pig small intestine Cell Tissue Res 235 285–93 Occurrence Handle10.1007/BF00217852 Occurrence Handle6367996
KN Bitar XX Zhu (1993) ArticleTitleExpression of bombesin-receptor subtypes and their differential regulation of colonic smooth muscle contraction Gastroenterology 105 1672–80 Occurrence Handle8253343
HR Fender PJ Curtis PL Rayford JC Thompson (1976) ArticleTitleEffect of bombesin on serum gastrin and cholecystokinin in dogs Surg Forum 27 414–6 Occurrence Handle1019921
P Plaisancié A Barcelo F Moro J Claustre JA Chayvialle JC Cuber (1998) ArticleTitleEffects of neurotransmitters, gut hormones and inflammatory mediators on mucus discharge in rat colon Am J Physiol Gastrointest Liver Physiol 275 1073–84
GT Cole AA Halawa EJ Anaissie (1996) ArticleTitleThe role of the gastrointestinal tract in hematogenous candidiasis: from the laboratory to the bedside Clin Infect Dis 22 73–88 Occurrence Handle8824969
F Meunier-Carpentier TE Kiehn D Armstrong (1981) ArticleTitleFungemia in the immunocompromised host. Changing patterns, antigenemia, high mortality Am J Med 71 363–70 Occurrence Handle10.1016/0002-9343(81)90162-5 Occurrence Handle6792913
Andrutis KA, Riggle PJ, Kumamoto CA, Tzipori S. Intestinal lesions associated with disseminated candidiasis in an experimental animal model. J Clin Microbiol 2000;2317–23
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Algin, C., Sahin, A., Kiraz, N. et al. Effectiveness of Bombesin and Saccharomyces boulardii Against the Translocation of Candida albicans in the Digestive Tract in Immunosuppressed Rats. Surg Today 35, 869–873 (2005). https://doi.org/10.1007/s00595-005-3049-9
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DOI: https://doi.org/10.1007/s00595-005-3049-9