Skip to main content
SpringerLink
Log in

Protective effects of royal jelly on the histomorphologic, oxidative stress and sperm parameters in Ofloxacin treated rat

  • Original Article
  • Published:
Comparative Clinical Pathology Aims and scope Submit manuscript

Abstract

Royal jelly is produced by worker bees as nutrition for bee larvae and adult queens and has also been shown to have protective effects against antibiotics. The aim of this investigation was to determine protective effect of royal jelly on the reproductive functions of male rats treated with ofloxacin. In this experiment, 32 mature male albino rats were randomly allocated into four groups (n = 8): control, ofloxacin only, royal jelly only, and ofloxacin with royal jelly. The results revealed that ofloxacin alone caused significant decreases (P < 0.05) in follicle-stimulating hormone, luteinizing hormone, testosterone, sperm count, sperm viability, total thiol molecules, and total antioxidant capacity compared to the control group. However, levels of immature sperm, DNA impaired sperm, malondialdehyde and nitric oxide were significantly increased (P < 0.05) in the ofloxacin group compared to the control. In the ofloxacin with royal jelly group, no significant increases or decreases were observed. Royal jelly has protective effects on reproductive function of male rat treated with ofloxacin.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Agarwal A, Sharma RK, Nallella KP, Thomas AJJR, Alvarez JG, Sikka SC (2006) Oxygen species as an independent marker of male factor infertility. Fertil Steril 86:878–885

    Article  CAS  PubMed  Google Scholar 

  • Aresh K, Marefat G, AmirAfshin K, Fatemeh F, Morteza K, Jafar H, Reza S (2009) Comparative study of the effect of gentamicin, neomycin, streptomycin: and ofloxacin antibiotics on sperm parameters and testis apoptosis in rat. Pak J Biol Sci 11(13):1683–1689

    Google Scholar 

  • Benzie IF, Strain JJ (1999) Ferric reducing/antioxidant power assay: direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods Enzymol 299:15–27

    Article  CAS  PubMed  Google Scholar 

  • Crotty KL, May R, Kulvicki A, Kumar D, Neal DE Jr (1995) The effect of antimicrobial therapy on testicular aspirate flow cytometry. J Urol 153:835–838

    Article  CAS  PubMed  Google Scholar 

  • Djurdjevic PT, Jelikic-Stankov M (1999) Study of solution equilibria between aluminium (III) ion and ofloxacin. J Pharm Biomed Anal 19:501–510

    Article  CAS  PubMed  Google Scholar 

  • Elnagar SA (2010) Royal jelly counteracts bucks “summer infertility”. Anim Reprod Sic 121:174–180

    Article  Google Scholar 

  • El-Nekeety AA, El-Kholy W, Abbas NF, Ebaid A, Amra HA, Abdel-Wahhab MA (2007) Efficacy of royal jelly against the oxidative stress of fumonisin in rats. Toxicon 50(2):256–269

    Article  CAS  PubMed  Google Scholar 

  • Eniseh Z, Gholamreza N, Vahid N, Reza H (2014) Protective effect of royal jelly on the sperm parameters and testosterone level and lipid peroxidation in adult mice treated with oxymetholone. AJP 4(1):43–52

    Google Scholar 

  • Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS, Tannenbaum SR (1982) Analysis of nitrate, nitrite, and [15 N] nitrate in biological fluids. Anal Biochem 126:131–138

    Article  CAS  PubMed  Google Scholar 

  • Hassan A (2009) Effect of royal jelly on sexual efficiency in adult male rats. Iraqi J Vet Sci 23:155–160

    Google Scholar 

  • Hu M, Dillared CJ (1994) Plasma SH and GSH measurement. Methods Enzymol 233:385–387

    Article  Google Scholar 

  • Jamnik P, Goranovič D, Raspor D (2007) Antioxidative action of royal jelly in the yeast cell. Exp Gerontol 42(7):594–600

    Article  CAS  PubMed  Google Scholar 

  • Kanbur M, Erasian G, Beyaz L, Silici S, Liman BC, Altinordulu S, Atasever A (2009) The effects of royal jelly on liver damage induced by paracetamol in mice. Exp Toxicol Pathol 61(2):123–132

    Article  CAS  PubMed  Google Scholar 

  • Kawahara S (1998) Chemotherapeutic agents under study. Nippon Rinsho December 56(12):3096–3099

    CAS  Google Scholar 

  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275

    CAS  PubMed  Google Scholar 

  • Majtán V, Majtánová L (1998) Post-antibiotic effect of some antibiotics on the metabolism of Pseudomonas Aeruginosa. J Basic Microbiol 38:221–227

    Article  PubMed  Google Scholar 

  • Matsumoto T, Takahashi K, Nagafuji T, Kubo S, Sakumoto M, Mochida O (1996) Fleroxacin enhancement of superoxide production by polymorphonuclear leukocytes: the role of protein kinases. Chemotherapy 42:280–285

    Article  CAS  PubMed  Google Scholar 

  • Montanari MP, Prenna M, Mingoia M, Ripa S, Varaldo PE (1998) In vitro antibacterial activity of trovafloxacin and five other fluoroquinolones. Chemotherapy 44:85–93

    Article  CAS  PubMed  Google Scholar 

  • Montvale NJ (1995) Physician’s desk reference 49th edition. Medical Economics Data Production Company 14:57–61

    Google Scholar 

  • Nagai T, Inoue R (2004) Preparation and the functional properties of water extract and alkaline extract of royal jelly. Food Cham 84:181–186

    Article  CAS  Google Scholar 

  • Nagai T, Inoue R, Suzuki N, Nagashima T (2006) Antioxidant properties of enzymatic hydrolysates from royal jelly. J Med Food 9:363–367

    Article  CAS  PubMed  Google Scholar 

  • Nakajima Y, Tsuruma K, Shimazawa M, Mishmi S, Hara H (2009) Comparison of bee products based on assays of antioxidant capacities. Complement Altern Med 9:1–4

    Article  Google Scholar 

  • Nelson JM, Chiller TM, Powers JH, Angulo FJ (2007) Fluoroquinolone-resistant campylobacter species and the withdrawal of fluoroquinolones from use in poultry: a public health success story. Clin Infect Dis 44(7):977–980

    Article  CAS  PubMed  Google Scholar 

  • Niehaus WG, Samuelsson JRB (1968) Formation of malonaldehyde from phospholipid arachidonate during microsomal lipid peroxidation. Eur J Biochem 6:126–130

    Article  CAS  PubMed  Google Scholar 

  • Seibert G, Limbert M, Klesel N (1983) Comparison of the antibacterial in vitro and in vivo activity of ofloxacin (HOE 280 DL 8280) and nalidixic acid analogues. Eur J Clin Microbiol 2:548–553

    Article  CAS  PubMed  Google Scholar 

  • Silici S, Ekmekcioglu O, Eraslan G, Demirtas A (2009) Antioxidative effect of royal jelly in cisplatin-induced testes damage. Urology 74:545–551

    Article  PubMed  Google Scholar 

  • Silici S, Ekmekcioglu O, Kanbur M, Deniz K (2010) The protective effect of royal jelly against cisplatin-induced renal oxidative stress in rats. World J Urol 29:127–132

    Article  PubMed  Google Scholar 

  • Tamura S, Kono T, Harada C, Yamaguchi K, Moriyama T (2009) Estimation and characterisation of major royal jelly proteins obtained from the honeybee Apismerifera. Food Chem 114:1491–1497

    Article  CAS  Google Scholar 

  • Tayebeh A, Gholamreza N, Vahid N (2014) Protective effect of royal jelly on fertility and biochemical parameters in bleomycin- induced male rats. Iran J Reprod Med March 12(3):209–216

    Google Scholar 

  • Taymour M (2010) Long-term ofloxacin testicular toxicity: an experimental study. Andrology 42(2):92–96

    Article  Google Scholar 

  • Thoung GM, Domarle O, Pocidalo J, Hayem G (1996) Effects of fluoroquinolones on cultured articular chondrocytes flow cytometric analysis of free radical production. J Pharmacol Exp Therap 271:1544–1549

    Google Scholar 

  • Virro MR, Larson-Cook KL, Evenson DP (2004) Sperm chromatin structure assay related to blastocyst rate, pregnancy rate and spontaneous abortion in IVF and ICSI cycles. Fertil Steril 8:1289–1295

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Basic Sciences, Farhangian University, Tehran, Iran

    Ghodrat Ebadi Manas

  2. Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

    Gholamreza Najafi

Authors
  1. Ghodrat Ebadi Manas
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Gholamreza Najafi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ghodrat Ebadi Manas.

Ethics declarations

The authors state that they have no conflict of interest. All investigation processes in this work were based on laws of the Research Ethics Committee for Research on Laboratory Animals Farhangain University of Urmia.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Manas, G.E., Najafi, G. Protective effects of royal jelly on the histomorphologic, oxidative stress and sperm parameters in Ofloxacin treated rat. Comp Clin Pathol 26, 1111–1115 (2017). https://doi.org/10.1007/s00580-017-2494-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00580-017-2494-3

Keywords

Search

Navigation