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Diversity of arbuscular mycorrhizal fungi in greenhouse soils continuously planted to watermelon in North China

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Abstract

In North China, watermelon is grown in commercial greenhouses in a continuous monoculture and with high application rates of manure or compost. The aim of this study was to determine how the diversity of arbuscular mycorrhizal fungi (AMF) in these soils changed over long periods (0 to 20 years) of monoculture. AMF in control soils (from fields not replanted with watermelon and located near the greenhouses) and in greenhouses (in Daxing, Beijing, and Weifang, Shandong) that had been continuously replanted with watermelon for 5, 10, 15, or 20 years (three greenhouses per year per location) were identified and quantified based on spore morphology and on denaturing gradient gel electrophoresis (DGGE). The total number of AMF species and genera were 13 and 3 in soils replanted for 5–20 years and 19 and 4 in control soils. AMF species richness (SR), the Shannon–Wiener index (H), and spore density declined as the number of years in which watermelon was replanted increased. The available phosphorus, potassium, and nitrogen in the soil increased as the number of years in which watermelon was replanted increased. Values for SR and H were higher when based on DGGE than on spore morphology. The results suggest that current greenhouse practices in North China reduce the AMF diversity in the soil.

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References

  • Antunes PM, Koch AM, Dunfield KE, Hart MM, Downing A, Rillig MC, Klironomos JN (2009) Influence of commercial inoculation with Glomus intraradices on the structure and functioning of an AM fungal community from an agricultural site. Plant Soil 317:257–266

    Article  CAS  Google Scholar 

  • Borstler B, Renker C, Kahmen A, Buscot F (2006) Species composition of arbuscular mycorrhizal fungi in two mountain meadows with differing management types and levels of plant biodiversity. Biol Fertil Soils 42:286–298

    Article  Google Scholar 

  • Collier SC, Yarnes CT, Herman RP (2003) Mycorrhizal dependency of Chihuahuan desert plants is influenced by life history strategy and root morphology. J Arid Environ 55:223–229

    Article  Google Scholar 

  • Cornejo P, Azcón-Aguilar C, Barea JM, Ferrol N (2004) Temporal temperature gradient gel electrophoresis (TTGE) as a tool for the characterization of arbuscular mycorrhizal fungi. FEMS Microbiol Lett 241:265–270

    Article  CAS  PubMed  Google Scholar 

  • Cuenca G, Andrade ZD, Lovera M, Fajardo L, Meneses E (2003) Mycorrhizal response of Clusia pusilla growing in two different soils in the field. Trees 17:200–206

    Google Scholar 

  • Dalpe Y (1993) Vesicular–arbuscular mycorrhiza. In: Carter MR (ed) Soil sampling and methods of analysis. Lewis Publishers, Boca Raton, pp 287–301

    Google Scholar 

  • Gai JP, Liu RJ (2003) Effects of soil factors on AMF in the rhizosphere of wild plants. Chin J Appl Ecol 14:18–22 (in Chinese, with an English abstract)

    Google Scholar 

  • Galván GA, Parádi I, Burger K, Baar J, Kuyper TW, Scholten OE, Kik C (2009) Molecular diversity of arbuscular mycorrhizal fungi in onion roots from organic and conventional farming systems in the Netherlands. Mycorrhiza 19:317–328

    Article  PubMed  PubMed Central  Google Scholar 

  • Helgason T, Daniell TJ, Husband R, Fitter AH, Yong JPW (1998) Ploughing the word-wide-web. Nature 394:431

    Article  CAS  PubMed  Google Scholar 

  • Jasper DA, Abbott LK, Robson AD (1989) Soil disturbance reduced the infectivity of external hyphae of vesicular–arbuscular mycorrhizal fungi. New Phytol 112:93–99

    Article  Google Scholar 

  • Johnson NC, Copeland PJ, Crookston RK, Pfleger FL (1992) Mycorrhiza: possible explanation for yield decline with continuous corn and soybean. Agron J 84:387–390

    Article  Google Scholar 

  • Kayal C, Higgs D, Kirnak H, Tas I (2003) Mycorrhizal colonisation improves fruit yield and water use efficiency in watermelon (Citrullus lanatus Thunb.) grown under well-watered and water-stressed conditions. Plant Soil 253:287–292

    Article  Google Scholar 

  • Kowalchuk GA, Gerards S, Woldendorp JW (1997) Detection and characterization of fungal infections of Ammophila arenaria (marram grass) roots by denaturing gradient gel electrophoresis of specifically amplified 18 S rDNA. Appl Environ Microbiol 63:3858–3865

    CAS  PubMed  PubMed Central  Google Scholar 

  • Li DC, Hua JM, Li ZP, Zhou X, Zhang TL, Velde B (2003) Changes in trace element contents in green-house soils and years of vegetable cultivation. Soils 35:495–499 (in Chinese, with an English abstract)

    CAS  Google Scholar 

  • Li M, Liu RJ, Li XL (2004) Influences of arbuscular mycorrhizal fungi on growth and Fusarium-wilt disease of watermelon in field. Acta Phytopathol Sinica 34(5):472–473 (in Chinese, with an English abstract)

    Google Scholar 

  • Li H, Sun AQ, Guo HJ (2010) Effects of different planting patterns on farmland soil quality in Yellow River alluvial plain of Shandong Province. Chinese J Appl Ecol 21:365–372 (in Chinese, with an English abstract)

    CAS  Google Scholar 

  • Liu RJ, Chen YL (2007) Mycorrhizology. Science Press, Beijing (in Chinese)

    Google Scholar 

  • Liu RJ, Jiao H, Li Y, Li M, Zhu XC (2009) Advances in the study of species diversity of arbuscular mycorrhizal fungi. Chinese J Appl Ecol 20:2301–2307 (in Chinese, with an English abstract)

    CAS  Google Scholar 

  • Lou YL, Guan LZ, Wang LL, Ku KW, He L (2007) Changes of pH and enzyme activities in soils for different tobacco cropping years. Plant Nutr Fertilizer Sci 13:531–534 (in Chinese, with an English abstract)

    CAS  Google Scholar 

  • Lugo MA, Ferrero M, Menoyo E, Estevez MC, Sineriz F, Anton A (2008) Arbuscular mycorrhizal fungi and rhizospheric bacteria diversity along an altitudinal gradient in south American puna grassland. Microb Ecol 55:705–713

    Article  CAS  PubMed  Google Scholar 

  • Neumann E, George E (2004) Colonisation with the arbuscular mycorrhizal fungus Glomus mosseae (Nicol. & Gerd.) enhanced phosphorus uptake from dry soil in Sorghum bicolor (L.). Plant Soil 261:245–255

    Article  CAS  Google Scholar 

  • Peng J, Li Y, Shi P, Chen X, Lin H, Zhao B (2011) The differential behavior of arbuscular mycorrhizal fungi in interaction with Astragalus sinicus L. under salt stress. Mycorrhiza 21:27–33

    Article  CAS  PubMed  Google Scholar 

  • Porter WM, Robson AD, Abbott LK (1987) Field survey of the distribution of vesicular- arbuscular mycorrhizal fungi in relation to soil pH. J Appl Ecol 24:659–662

    Article  Google Scholar 

  • Schenck NC, Perez Y (1988) Manual for the Identification of VA Mycorrhizal Fungi. Synergistic Publications, Gainesville, Florida

    Google Scholar 

  • Schwarzott D, Schüßler A (2001) A simple and reliable method for SSU rRNA gene DNA extraction, amplification and cloning from single AM fungal spores. Mycorrhiza 10:203–207

    Article  CAS  Google Scholar 

  • Shi ZY, Diao ZK, Xu Q, Li M, Liu RJ (2006) Effects of media and arbuscular mycorrhizal fungi on growth and yield of watermelon. J Laiyang Agric College (Natural Science) 23(1):1–6

    Google Scholar 

  • Simon L, Lalonde M, Bruns TD (1992) Specific amplification of 18S fungal ribosomal genes from vesicular–arbuscular endomycorrhizal fungi colonizing roots. Appl Environ Microbiol 58:291–295

    CAS  PubMed  PubMed Central  Google Scholar 

  • Smith SE, Read DJ (1997) Mycorrhizal symbiosis, 2nd edn. Academic, London

    Google Scholar 

  • Subramanian KS, Santhanakrishnan P, Balasubramanian P (2006) Responses of field grown tomato plants to arbuscular mycorrhizal fungal colonization under varying intensities of drought stress. Sci Hortic 107:245–253

    Article  Google Scholar 

  • Sýkorová Z, Ineichen K, Wiemken A, Redecker D (2007) The cultivation bias: different communities of arbuscular mycorrhizal fungi detected in roots from the field, from bait plants transplanted to the field, and from a greenhouse trap experiment. Mycorrhiza 18:1–14

    Article  PubMed  Google Scholar 

  • Tawaraya K, Saito M, Morioka M, Waqatsuma T (1994) Effect of phosphate application to arbuscular mycorrhizal onion on the development and succinate dehydrogenase activity of internal hyphae. Soil Sci Plant Nutr 40:667–673

    Article  CAS  Google Scholar 

  • Wang GM, Stribley DP, Tinker PB, Walker C (1993) Effects of pH on arbuscular mycorrhiza I. Field observations on the long-term liming experiments at Rothamsted and Woburn. New Phytol 124:465–472

    Article  CAS  Google Scholar 

  • Wang FY, Liu RJ, Lin XG, Zhou JM (2003) Comparison of diversity of arbuscular mycorrhizal fungi in different ecological environments. Acta Ecologica Sinica 23:2666–2671 (in Chinese, with an English abstract)

    Google Scholar 

  • Wang MY, Diao ZK, Liang MX, Liu RJ (2005) Advances in the study of AM fungal diversity in agroecosystems. Acta Ecologica Sinica 25:2544–2549 (in Chinese, with an English abstract)

    Google Scholar 

  • Yang JL, Zhang GL (2003) Quantitative relationship between land use and phosphorus discharge in subtropical hilly regions of China. Pedosphere 13:67–74

    CAS  Google Scholar 

  • Yeates C, Gilling MR, Davison AD, Altavilla N, Veal DA (1998) Methods for microbial DNA extraction from soil for PCR amplification. Biol Proced Online 1:40–47

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Zhang MQ, Wang YS, Xing LJ (1999) The relationship between the distribution of AM fungi and environmental factors. Mycosystema 18:25–29 (in Chinese, with an English abstract)

    Google Scholar 

  • Zhao GX, Li XJ, Wang RY, Li T, Yue YD (2007) Soil nutrients in intensive agricultural areas with different land-use types in Qingzhou County, China. Pedosphere 17:165–171

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors are grateful to Dr. Ian Riley and Professor Bruce Jaffee for their valuable comments. This work was financially supported by the National Natural Science Foundation of China (30871737); the Open 2010 Foundation of the State Key Laboratory of Soil and Sustainable Agriculture, the Institute of Soil Science, Chinese Academy of Sciences; and the Qingdao Natural Science Foundation (08-1-3-20-jch).

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Authors and Affiliations

  1. Institute of Mycorrhizal Biotechnology, Qingdao Agricultural University, Qingdao, 266109, China

    Hui Jiao & Runjin Liu

  2. School of Earth and Environment (M087), The University of Western Australia, Perth, WA, 6009, Australia

    Yinglong Chen

  3. State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China

    Xiangui Lin

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  1. Hui Jiao
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  2. Yinglong Chen
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  4. Runjin Liu
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Correspondence to Runjin Liu.

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Jiao, H., Chen, Y., Lin, X. et al. Diversity of arbuscular mycorrhizal fungi in greenhouse soils continuously planted to watermelon in North China. Mycorrhiza 21, 681–688 (2011). https://doi.org/10.1007/s00572-011-0377-z

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  • DOI: https://doi.org/10.1007/s00572-011-0377-z

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