Abstract
Three orchid genera, Paphiopedilum, Cymbidium, and Dendrobium, are among the most heavily traded ornamental plants in Thailand. In this study, 27 isolates of Rhizoctonia-like fungi were isolated from root sections of mature orchids in the three orchid genera, collected from diverse horticultural settings in Chiang Mai and Chiang Rai provinces of Thailand. Fungal identification was done by the morphological characterization, the comparison of the internal transcribed spacer and 5.8S ribosomal DNA sequences, and the phylogenetic analysis. Epulorhiza repens was found to be the most common species found in the roots of various species of all three orchid genera, whereas Epulorhiza calendulina-like isolates were strictly found in the roots of Paphiopedilum species. We have also isolated and described an anamorph of Tulasnella irregularis, four new anamorphic species in the genus Tulasnella, and a new anamorphic species in the family Tulasnellaceae. Our study provides information on diversity of root-associated fungi of the orchid genera and at the sampling sites that were rarely addressed in the previous studies.
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Acknowledgments
We thank Ms. Kalaya Konkwaew, Mae Klang Watershed Management Unit, Mr. Tosaporn Thanamee and Mr. Natthawut Maneeratanachaiyong, P. villosum conservation project, Mr. Auttapon Taluengjit, and Mr. Kittchai Phulek for providing the orchid roots; Dr. Kevin Hyde, Mae Fah Luang University, and Dr. Eakaphun Bangyeekhun, Silpakorn University, for their advice on fungal isolation and storage; Dr. Tosak Seelanan, Chulalongkorn University, and Mr. Sitthisack Phoulivong for their guidance on the phylogenetic analysis. The Thailand Research Fund and the Office of the Higher Education Commission are gratefully acknowledged for the financial support.
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Nontachaiyapoom, S., Sasirat, S. & Manoch, L. Isolation and identification of Rhizoctonia-like fungi from roots of three orchid genera, Paphiopedilum, Dendrobium, and Cymbidium, collected in Chiang Rai and Chiang Mai provinces of Thailand. Mycorrhiza 20, 459–471 (2010). https://doi.org/10.1007/s00572-010-0297-3
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DOI: https://doi.org/10.1007/s00572-010-0297-3