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Arbuscular mycorrhiza partially protect chicory roots against oxidative stress induced by two fungicides, fenpropimorph and fenhexamid

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Abstract

The present work examined the oxidative stress induced by different concentrations (0.02 and 0.2 mg l-1) of two sterol biosynthesis inhibitor fungicides (fenpropimorph and fenhexamid) in non-target chicory root colonised or not by Glomus intraradices in a monoxenic system. The fungicides were found to cause oxidative damage by increasing lipid peroxidation measured by malondialdehyde production in non-colonised roots. Detoxification of the H2O2 product was measured at 0.2 mg l-1 of fenpropimorph by an increase in peroxidase activities suggesting an antioxidant capacity in these roots. Moreover, this study pointed out the ability of arbuscular mycorrhiza to alleviate partially the oxidative stress in chicory roots, probably by lowering reactive oxygen species concentrations, resulting from increases in antioxidant defences. Our results suggest that the enhanced fungicide tolerance in the AM symbiosis could be related to less cell membrane damage.

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Acknowledgements

In this paper, the first position is shared by the two co-authors: A. Lounès-Hadj Sahraoui and E. Campagnac. This work was supported by a Marie-Curie Early stage Research Training Fellowship of the European Community’s Sixth framework Programme under contract number MEST-CT-2004-514213. The laboratory participates in the Institut de Recherches en Environnement Industriel (IRENI), which is financed by the Communauté Urbaine de Dunkerque, the Région Nord-Pas de Calais, the Ministère de l’enseignement supérieur et de la recherche, and European funds (FEDER). We thank Bayer and BASF, which kindly provided the SBI fungicides, and we are grateful to Natacha Bourdon for technical help.

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  1. Univ Lille Nord de France, Lille, 59000, France

    Estelle Campagnac, Anissa Lounès-Hadj Sahraoui, Djouher Debiane, Joël Fontaine, Frédéric Laruelle, Guillaume Garçon, Anthony Verdin, Roger Durand, Pirouz Shirali & Anne Grandmougin-Ferjani

  2. Laboratoire de Mycologie/Phytopathologie/Environnement, ULCO, Calais, 62228, France

    Estelle Campagnac, Anissa Lounès-Hadj Sahraoui, Djouher Debiane, Joël Fontaine, Frédéric Laruelle, Roger Durand & Anne Grandmougin-Ferjani

  3. Laboratoire Catalyse et Environnement, ULCO, Dunkerque, 59140, France

    Guillaume Garçon, Anthony Verdin & Pirouz Shirali

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  1. Estelle Campagnac
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Campagnac, E., Lounès-Hadj Sahraoui, A., Debiane, D. et al. Arbuscular mycorrhiza partially protect chicory roots against oxidative stress induced by two fungicides, fenpropimorph and fenhexamid. Mycorrhiza 20, 167–178 (2010). https://doi.org/10.1007/s00572-009-0267-9

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