Abstract
This paper reports the first application of poly-tetrafluoroethylene (PTFE) microstructure, which fabricated by synchrotron radiation induced photo-evaporation process, to enzyme-linked immunosorbent assay. The advantages of PTFE microstructure for the development of lab-on-a-chip due to the extremely high-aspect ratio microstructure and chemical stability of PTFE is discussed. The results of immunoassay shows the successful detection of analyte (mouse IgG) with detection range with 0–100 ng/ml. This result suggests the successful immobilization of antibody (anti-mouse IgG goat antibody) onto the X-ray exposed surface of PTFE microstructure and successful demonstration of antigen–antibody reaction in the PTFE microstructure. We also demonstrated the detection of polychlorinated biphenyl (PCB). As the result of demonstration, we successfully detected PCB with ranging analyte concentration of 0.1–10 ng/ml.
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Ukita, Y., Kondo, S., Kataoka, C. et al. Immunoassay using poly-tetrafluoroethylene microstructure in organic solvent. Microsyst Technol 16, 1465–1470 (2010). https://doi.org/10.1007/s00542-010-1063-3
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DOI: https://doi.org/10.1007/s00542-010-1063-3