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Characteristics of rat bone marrow cells differentiated into a liver cell lineage and dynamics of the transplanted cells in the injured liver

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Abstract

Background

Bone marrow cells (BMCs) have been shown to differentiate into a liver cell lineage, but little is known about their dynamics following transplantation. BMCs were cultured to investigate the expression of liver-specific genes in vitro and transplanted into in vivo liver-injury models to elucidate their dynamics in the liver.

Methods

The mRNA expression of various liver-specific genes in BMCs cocultured with hepatocytes was analyzed using reverse transcription-polymerase chain reaction. BMCs from transgenic rats expressing green fiuorescent protein were transplanted into the spleen of rat liver-injury models induced with 2-acetylaminofiuorene (2-AAF) or carbon tetrachloride (CCl4). BMCs were also transplanted directly into livers treated with CCl4 to determine which route is better for transplantation.

Results

BMCs differentiated into a liver cell lineage in vitro and expressed mRNAs consistent with mature hepatocytes, including albumin. The transplanted BMCs were found in the liver in the CCl4-induced injury model, but not in the 2-AAF-induced model. The hepatocyte growth factor and fibroblast growth factor mRNA levels in the liver were significantly higher in the CCl4-induced model than in the 2-AAF-induced model. Migration of BMCs to the liver was more effective following injection into the liver, rather than into the spleen.

Conclusions

Cultured BMCs differentiated into a liver cell lineage are a potential source for cell transplantation. Transplantation is successful in the severely injured liver with a high level of expression of mRNAs for growth factors. Injection of BMCs directly into the liver is the preferred route of administration.

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Authors and Affiliations

  1. Department of Gastroenterology, Course of Internal Medicine and Therapeutics, Yamagata University School of Medicine, Yamagata, 990-9585, Japan

    Kazuo Okumoto, Takafumi Saito, Hiroaki Haga, Etsuko Hattori, Rika Ishii, Tetsuru Karasawa, Akihiko Suzuki, Keiko Misawa, Mai Sanjo, Jun-itsu Ito, Kazuhiko Sugahara, Koji Saito, Hitoshi Togashi & Sumio Kawata

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  1. Kazuo Okumoto
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  2. Takafumi Saito
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Okumoto, K., Saito, T., Haga, H. et al. Characteristics of rat bone marrow cells differentiated into a liver cell lineage and dynamics of the transplanted cells in the injured liver. J Gastroenterol 41, 62–69 (2006). https://doi.org/10.1007/s00535-005-1723-8

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  • DOI: https://doi.org/10.1007/s00535-005-1723-8

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