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Cilastatin does not affect Carba NP test performance for detection of carbapenemase production in Enterobacteriaceae

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Summary

The Carba NP test is a simple confirmation method for carbapenemase-producing Enterobacteriaceae (CPE) but reagents have to be freshly prepared as imipenem sodium salt is unstable. We evaluated the Carba NP test performance based on a commercially available 10-fold cheaper drug formulation containing cilastatin against 217 CPE and 78 non-CPE isolates with reduced meropenem susceptibility. Specificity and sensitivity were 100 % and 98.6 %, respectively and 3 false negative results of blaVIM-1-producing Proteus mirabilis were reproducible with the RAPIDEC® Carba NP test. Cilastatin does not disturb test performance provided that the imipenem drug quantity is doubled.

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Acknowledgements

We would like to thank all Austrian microbiology laboratories for referring suspicious strains to the National Reference Laboratory for nosocomial infection and antimicrobial resistance (NRZ) in Linz and thus for providing a local basis strain collection of CPE to the NRZ.

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Authors and Affiliations

  1. Institute of Hygiene, Microbiology and Tropical Medicine, National Reference Centre for Nosocomial Infections and Antimicrobial Resistance, Elisabethinen Hospital Linz, Fadingerstraße 1, 4020, Linz, Austria

    Rainer Hartl,  Heidrun Kerschner,  Petra Apfalter &  Rainer Gattringer

  2. analyse BioLab, Linz, Austria

    Sarah Widhalm

Authors
  1. Rainer Hartl
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  2. Sarah Widhalm
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  3. Heidrun Kerschner
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  4. Petra Apfalter
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  5. Rainer Gattringer
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Correspondence to Rainer Gattringer.

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Conflict of interest

R. Hartl, S. Widhalm, H. Kerschner, P. Apfalter, and R. Gattringer declare that they have no competing interests.

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Hartl, R., Widhalm, S., Kerschner, H. et al. Cilastatin does not affect Carba NP test performance for detection of carbapenemase production in Enterobacteriaceae. Wien Klin Wochenschr 129, 29–32 (2017). https://doi.org/10.1007/s00508-016-1106-6

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  • DOI: https://doi.org/10.1007/s00508-016-1106-6

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