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cDNA microarray analysis of gene expression changes during pollination, pollen-tube elongation, fertilization, and early embryogenesis in rice pistils

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Abstract

During the processes of pollen recognition, pollen-tube guidance, fertilization, and early embryogenesis, a complicated gene network is highly regulated in plants. To visualize the profile of gene expression during these processes, we performed cDNA microarray analysis with RNA isolated from rice pistils at several stages. We identified 32 cDNA clones, in 21 non-redundant groups, with pistil-specific expression patterns. These genes could be clustered into five groups based on expression patterns, and included pathogenesis-related genes, transcription factors, and genes related to pollen germination, pollen tube elongation, and starch degradation. The microarray analysis identified several previously undescribed genes that are expressed in a pistil-specific manner. These results provide new clues to the molecular mechanisms that underlie plant reproduction.

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References

  • Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic alignment search tool. J Mol Biol 215:403–410

    Article  CAS  PubMed  Google Scholar 

  • Atkinson AH, Heath RL, Simpson RJ, Clarke AE, Anderson MA (1993) Proteinase inhibitors in Nicotiana alata stigmas are derived from a precursor protein which is processed into five homologous inhibitors. Plant Cell 5:203–213

    Article  Google Scholar 

  • Baltz R, Schmit AC, Kohnen M, Hentges F, Steinmetz A (1999) Differential localization of the LIM domain protein PLIM-1 in microspores and mature pollen grains from sunflower. Sex Plant Reprod 12:60–65

    Article  Google Scholar 

  • Bell J, Hicks G (1976) Transmitting tissue in the pistil of tobacco: light and electron microscopic observations. Planta 131:187–200

    Article  Google Scholar 

  • Constabel CP, Brisson N (1995) Stigma- and vascular-specific expression of the PR-10a gene of potato: a novel pattern of expression of a pathogenesis-related gene. Mol Plant Microbe Interact 8:104–113

    Google Scholar 

  • Crosgrove DJ, Bedinger P, Durachko DM (1997) Group I allergens of grass pollen as cell wall-loosening agents. Proc Natl Acad Sci USA 94:6559–6564

    Google Scholar 

  • Datta R, Chamusco KC, Chourey PS (2002) Starch biosynthesis during pollen maturation is associated with altered patterns of gene expression in maize. Plant Physiol 130:1645–1656

    Article  Google Scholar 

  • Davies C, Robinson SP (2000) Differential screening indicates a dramatic change in mRNA profiles during grape berry ripening. Cloning and characterization of cDNAs encoding putative cell wall and stress response proteins. Plant Physiol 122:803–812

    Article  CAS  PubMed  Google Scholar 

  • Eldik GJ van, Wingens M, Ruiter RK, van Herpen MMA (1996) Molecular analysis of a pistil-specific gene expressed in the stigma and stylar cortex of Solanum tuberosum. Plant Mol Biol 30:171–176

    Article  Google Scholar 

  • Eliasson A, Gass N, Mundel C, Baltz R, Krauter R, Evrard JL, Steinmetz A (2000) Molecular and expression analysis of a LIM protein gene family from flowering plants. Mol Gen Genet 264:257–267

    Article  Google Scholar 

  • Endo M, Tsuchiya T, Saito H, Matsubara H, Hakozaki H, Masuko H, Kamada M, Higashitani A, Takahashi H, Fukuda H, Demura T, Watanabe M (2004) Identification and molecular characterization of novel anther-specific genes in japonica rice, Oryza sativa L. by using cDNA microarray. Genes Genet Syst 79:213–226

    Article  Google Scholar 

  • Favaro R, Pinyopich A, Battaglia R, Kooiker M, Borghi L, Ditta G, Yanofsky MF, Kater MM, Colombo L (2003) MADS-box protein complexes control carpel and ovule development in Arabidopsis. Plant Cell 15:2603–2611

    Article  CAS  PubMed  Google Scholar 

  • Fischer RL, Bennet AB (1991) Role of cell wall hydrolases in fruit ripening. Annu Rev Plant Physiol Plant Mol Biol 42:675–703

    Article  CAS  Google Scholar 

  • Grebe M, Gadea J, Steinmann T, Kientz M, Rahfeld JU, Salchert K, Koncz C, Jurgens G (2000) A conserved domain of the Arabidopsis GNOM protein mediates subunit interaction and cyclophilin 5 binding. Plant Cell 12:343–356

    Google Scholar 

  • Harikrishna K, Jampates–Beale R, Milligan SB, Gasser CS (1996) An endochitinase gene expressed at high levels in the stylar transmitting tissue of tomatoes. Plant Mol Biol 30:899–911

    Article  Google Scholar 

  • Hoshikawa K (1993) Anthesis, fertilization and development of caryopsis. In: Matsuo T, Hoshikawa K (eds) Science of the rice plant (morphology) vol 1. Food and Agriculture Policy Research Center, Tokyo, pp 339–376

    Google Scholar 

  • Jansen MAK, Sessa G, Malkin S, Fluhr R (1992) PEPC-mediated carbon fixation in transmitting track cells reflects style-pollen tube interactions. Plant J 2:507–515

    Google Scholar 

  • Jeon JS, Lee S, Jung KH, Yang WS, Yi GH, Oh BG, An G (2000) Production of transgenic rice plants showing reduced heading date and plant height by ectopic expression of rice MADS-box genes. Mol Breed 6:581–592

    CAS  Google Scholar 

  • Kawaoka A, Ebinuma H (2001) Transcriptional control of lignin biosynthesis by tobacco LIM protein. Phytochemistry 57:1149–1157

    Article  Google Scholar 

  • Kawaoka A, Kaothien P, Yoshida K, Endo S, Yamada K, Ebinuma H (2000) Functional analysis of tobacco LIM protein Ntlim1 involved in lignin biosynthesis. Plant J 22:289–301

    Article  CAS  PubMed  Google Scholar 

  • Kuboyama T, Yoshida KT, Takeda G (1997) An acidic 39-kDa protein secreted from stigmas of tobacco has an amino-terminal motif that is conserved among thaumatin-like proteins. Plant Cell Physiol 38:91–95

    Google Scholar 

  • Lee S, Kim J, Son JS, Nam J, Jeong DH, Lee K, Jang S, Yoo J, Lee J, Lee DY, Kang HG, An G (2003) Systematic reverse genetic screening of T-DNA tagged genes in rice for functional genomic analyses: MADS-box genes as a test case. Plant Cell Physiol 44:1403–1411

    Article  Google Scholar 

  • Lee Y, Kende H (2001) Expression of β-expansins is correlated with internodal elongation in deepwater rice. Plant Physiol 127:645–654

    Article  Google Scholar 

  • Lee Y, Choi D, Kende H (2001) Expansins: ever-expanding numbers and function. Curr Opin Plant Biol 4:527–532

    Article  CAS  PubMed  Google Scholar 

  • Mahalingam R, Wang G, Knap HT (1999) Polygalacturonase and polygalacturonase inhibitor protein: gene isolation and transcription in Glycine max-Heterodera glycines interactions. Mol Plant Microbe Interact 6:490–498

    Google Scholar 

  • Malkin R, Niyogi K (2000) Photosynthesis. In: Buchanan BB, Gruissem W, Jones RL (eds) Biochemistry and Molecular Biology of Plants. American Society of Plant Physiologists, Rockville, Md., pp 568–629

    Google Scholar 

  • Markovic O, Jornvall H (1986) Pectinesterase. The primary structure of the tomato enzyme. Eur J Biochem 158:455–462

    Google Scholar 

  • Mosolov VV, Grigor’eva LI, Valueva TA (2001) Involvement of proteolytic enzymes and their inhibitors in plant protection (review). Appl Biochem Microbiol 37:115–123

    Article  Google Scholar 

  • Mu JH, Stains JP, Kao TH (1994) Characterization of a pollen-expressed gene encoding a putative pectin esterase of Petunia inflata. Plant Mol Biol 25:539–544

    CAS  PubMed  Google Scholar 

  • Nakazono M, Yoshida KT (1997) A rapid and efficient method for the isolation of differentially expressed genes: simplified differential display. Plant Biotechnol 14:187–190

    Google Scholar 

  • Ng M, Yanofsky MF (2001) Function and evolution of the plant MADS-box gene family. Nat Rev Genet 2:186–195

    Google Scholar 

  • Ori N, Sessa G, Lotan T, Himmelhoch S, Fluhr R (1990) A major stylar matrix polypeptide (sp41) is a member of the pathogenesis-related proteins superclass. EMBO J 9:3429–3436

    CAS  PubMed  Google Scholar 

  • Powell ALT, van Kan J, ten Have A, Visser J, Greve LC, Bennett AB, Labavitch JM (2000) Transgenic expression of pear PGIP in tomato limits fungal colonization. Mol Plant Microbe Interact 9:942–950

    Google Scholar 

  • Romano PGN, Horton P, Gray JE (2004) The Arabidopsis cyclophilin gene family. Plant Physiol 134:1–15

    Google Scholar 

  • Sassa H, Hirano H (1998) Style-specific and developmentally regulated accumulation of a glycosylated thaumain/PR5-like protein in Japanese pear (Pyrus serotina Rehd.) Planta 205:514–521

    Article  Google Scholar 

  • Smith AM, Zeeman SC, Thorneycroft D, Smith SM (2003) Starch mobilization in leaves. J Exp Bot 54:577–583

    Article  Google Scholar 

  • Vidali L, Hepler PK (2001) Actin and pollen tube growth. Protoplasma 215:64–76

    Google Scholar 

  • Yazaki J, Kishimoto N, Nakamura K, Fujii F, Shimbo K, Otsuka Y, Wu J, Yamamoto K, Sakata K, Sasaki T, Kikuchi S (2000) Embarking on rice functional genomics via cDNA microarray: use of 3′ UTR probes for specific gene expression analysis. DNA Res 7:367–370

    Google Scholar 

  • Yoshida KT, Kuboyama T (1997) Isolation of sexual reproduction-related genes in rice by simplified differential display. Rice Genet Newslett 14:126–129

    Google Scholar 

  • Yoshida KT, Kuboyama T (2001) A subtilisin-like serine protease specifically expressed in reproductive organs in rice. Sex Plant Reprod 13:193–199

    Google Scholar 

  • Yoshida KT, Naito S, Takeda G (1994) cDNA cloning of regeneration-specific genes in rice by differential screening of randomly amplified cDNAs using RAPD primers. Plant Cell Physiol 35:1003–1009

    Google Scholar 

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Acknowledgements

The authors are grateful to Dr. Hiroyuki Hirano and Dr. Nobuhiro Tsutsumi (The University of Tokyo) for valuable discussions. This work was supported in part by a grant from the Ministry of Agriculture, Forestry, and Fisheries of Japan (Rice Genome Project) to K.T.Y. and in part by Grant-in Aid from the Ministry of Education, Science, Sports, and Culture of Japan (13660004 and 15380004 to K.T.Y.).

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Authors and Affiliations

  1. Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan

    Kaoru T. Yoshida & Mikio Nakazono

  2. Faculty of Agriculture, Iwate University, Morioka, 020-8550, Japan

    Makoto Endo & Masao Watanabe

  3. Graduate School of Life Sciences, Tohoku University, Sendai, 980-8577, Japan

    Makoto Endo

  4. Graduate School of Science, The University of Tokyo, Tokyo, 113-0033, Japan

    Hiroo Fukuda

  5. Plant Science Center, The Institute of Physical and Chemical Research (RIKEN), Yokohama, 230-0045, Japan

    Hiroo Fukuda & Taku Demura

  6. Life Science Research Center, Mie University, Tsu, 514-8507, Japan

    Tohru Tsuchiya

Authors
  1. Kaoru T. Yoshida
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  2. Makoto Endo
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  3. Mikio Nakazono
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  4. Hiroo Fukuda
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  5. Taku Demura
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  6. Tohru Tsuchiya
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  7. Masao Watanabe
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Correspondence to Kaoru T. Yoshida.

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Yoshida, K.T., Endo, M., Nakazono, M. et al. cDNA microarray analysis of gene expression changes during pollination, pollen-tube elongation, fertilization, and early embryogenesis in rice pistils. Sex Plant Reprod 17, 269–275 (2005). https://doi.org/10.1007/s00497-004-0238-4

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  • DOI: https://doi.org/10.1007/s00497-004-0238-4

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