Abstract
Key message
An efficient micropropagation procedure for Rhus chinensis was developed using root segment-derived juvenile shoots as the source of materials through multiple shoot induction and hydroponic acclimation.
Abstract
Rhus chinensis Mill. is an economically important multipurpose tree in the Anacardiaceae that has a wide range of medicinal, ornamental, and industrial values. Here, an efficient micropropagation procedure for R. chinensis was developed using leaves, nodal segments, and petioles of juvenile shoots obtained from root segments as explants. The highest shoot induction frequency (100%) with a mean number of shoots (20.0 ± 0.9) was obtained when in vitro nodal segments were cultured on MS medium supplemented with 0.2 mg l−1 TDZ. The shoot clusters were transferred to medium containing 0.2 mg l−1 TDZ and 0.1 mg l−1 NAA for multiplication, and shoot elongation was observed on medium with 0.2 mg l−1 6-BA and 0.2 mg l−1 GA3. The highest rooting frequency (91.1%) was achieved on 1/4 MS medium fortified with 2.0 mg l−1 NAA. The major challenge of the low transplantation survival rate of regenerated plants was solved by adopting an ex vitro hydroponic acclimation system. This is the first report on the propagation of R. chinensis through direct shoot organogenesis and hydroponic acclimation. The present procedure provides a novel method for the large-scale propagation and germplasm conservation of R. chinensis.
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Funding
The study was financially supported by the National Natural Science Foundation of China (32201521), the Youth Innovation Promotion Association CAS (Grant No. 2021447), the Key Research and Development Program of Anhui Province (202204c06020045), the Chinese Academy of Sciences-Henan Province Achievement transfer and Transformation Project (2022110, 2022211, and 2021P02), the Grant of the President Foundation of Hefei Institutes of Physical Science of Chinese Academy of Sciences (YZJJZX202013), and the Strategic Biological Resources Planning Project of the Chinese Academy of Sciences (KFJBRP-007-014).
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Hou, J., Ding, S., Su, P. et al. Efficient plant regeneration mediated by root segment-derived juvenile shoots of Rhus chinensis Mill. Trees 37, 1055–1067 (2023). https://doi.org/10.1007/s00468-023-02405-5
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DOI: https://doi.org/10.1007/s00468-023-02405-5