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CD80 and suPAR in patients with minimal change disease and focal segmental glomerulosclerosis: diagnostic and pathogenic significance

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Abstract

Background

Minimal change disease (MCD) is characterized by increased urinary excretion of CD80, whereas focal segmental glomerulosclerosis (FSGS) is associated with increased serum soluble urokinase-type plasminogen activator receptor (suPAR). The aim of the study was to assess whether the simultaneous measurement of urinary CD80 and serum suPAR helps differentiate MCD and FSGS.

Methods

Urine and sera were collected from patients with MCD in relapse or in remission, from FSGS patients with nephrotic syndrome, and from healthy individuals. CD80 and suPAR were measured by ELISA.

Results

Urinary CD80 was significantly increased in MCD patients in relapse compared with those in remission and with FSGS patients and control individuals. Serum suPAR levels were significantly higher in patients with FSGS when compared with MCD patients in relapse. Urinary suPAR showed a positive correlation with proteinuria in MCD in relapse and FSGS patients, whereas urinary CD80 correlated with proteinuria only in MCD patients in relapse.

Conclusion

Urinary CD80 is elevated in MCD patients in relapse compared with FSGS patients. In contrast, serum suPAR is significantly elevated in FSGS patients. The consistent pattern of these two biomarkers in MCD and FSGS suggests that these two conditions represent different entities rather than a continuum spectrum of one disease.

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Acknowledgments

This study was supported by NIH R01DK080764 to EG and in part by grants from the National Institutes of Health DK073495 and DK089394 to JR

Disclosures

JR and CW are inventors of pending (JR, CW) and issued (JR) patents on novel technologies around proteinuric kidney diseases and stand to gain royalties from their commercialization. The other authors declare no conflict of interest.

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Authors and Affiliations

  1. Division of Pediatric Nephrology, Department of Pediatrics, University of Florida, 1600 SW Archer Rd. HD214, Gainesville, FL, 32610, USA

    Gabriel Cara-Fuentes & Eduardo H. Garin

  2. Department of Internal Medicine, Rush University Medical Center, Chicago, IL, USA

    Changli Wei & Jochen Reiser

  3. Division of Nephrology, Hospital Vall d’Hebron, Barcelona, Spain

    Alfons Segarra

  4. Division of Renal Diseases and Hypertension, Department of Medicine, University of Colorado, Denver, CO, USA

    Takuji Ishimoto, Christopher Rivard & Richard J. Johnson

Authors
  1. Gabriel Cara-Fuentes
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  2. Changli Wei
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  3. Alfons Segarra
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  4. Takuji Ishimoto
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  5. Christopher Rivard
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  6. Richard J. Johnson
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  7. Jochen Reiser
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  8. Eduardo H. Garin
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Corresponding author

Correspondence to Eduardo H. Garin.

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Cara-Fuentes, G., Wei, C., Segarra, A. et al. CD80 and suPAR in patients with minimal change disease and focal segmental glomerulosclerosis: diagnostic and pathogenic significance. Pediatr Nephrol 29, 1363–1371 (2014). https://doi.org/10.1007/s00467-013-2679-1

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  • DOI: https://doi.org/10.1007/s00467-013-2679-1

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