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Oxygen mass transfer enhancement by activated carbon particles in xylose fermentation media

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Abstract

In this work, the effect of activated carbon particles on the production of xylonic acid from xylose by Gluconobacter oxydans in a stirred tank bioreactor was investigated. The enhancement of the oxygen transfer coefficient by activated carbon particles was experimentally evaluated under different solids volume fractions, agitation and aeration rates conditions. The experimental conditions optimized by response surface methodology (agitation speed 800 rpm, aeration rate 7 L min−1, and activated carbon 0.002%) showed a maximum oxygen transfer coefficient of 520.7 h−1, 40.4% higher than the control runs without activated carbon particles. Under the maximum oxygen transfer coefficient condition, the xylonic acid titer reached 108.2 g/L with a volumetric productivity of 13.53 g L−1 h−1 and a specific productivity of 6.52 g/gx/h. In conclusion, the addition of activated carbon particles effectively enhanced the oxygen mass transfer rate. These results demonstrate that activated carbon particles enhanced cultivation for xylonic acid production an inexpensive and attractive alternative.

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Acknowledgements

This work was funded by the National key research and development program (2021YFC2101602), the National Natural Science Foundation of China (No. 22208160) and the Natural Science Foundation of the Jiangsu Higher Education Institutions of China (No. 21KJB530015).

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Authors and Affiliations

  1. Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Jiangsu Provincial Key Lab for the Chemistry and Utilization of Agro-Forest Biomass, College of ChemicalEngineering, Nanjing Forestry University, Nanjing, 210037, China

    Chenrong Ding, Chaozhong Xu, Tao He, Xu Liu, Yafei Zhu, Liqun Sun, Jia Ouyang & Xiaoli Gu

  2. Jiangsu Key Lab of Biomass-Based Green Fuels and Chemicals, Nanjing, 210037, People’s Republic of China

    Chenrong Ding, Chaozhong Xu, Tao He, Xu Liu, Yafei Zhu, Liqun Sun, Jia Ouyang & Xiaoli Gu

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  1. Chenrong Ding
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  2. Chaozhong Xu
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  3. Tao He
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  4. Xu Liu
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  5. Yafei Zhu
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  6. Liqun Sun
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  7. Jia Ouyang
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  8. Xiaoli Gu
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Contributions

CD: Methodology, Software, Validation, Formal analysis, Investigation, Data Curation, Writing-original draft. CX: Conceptualization, Methodology, Software, Validation, Formal analysis, Investigation, Data Curation, Writing-original draft, Writing-review & editing, Visualization, Supervision, Project administration, Funding acquisition. TH: Validation, Investigation, Data Curation. XL: Formal analysis. YZ: Formal analysis. LS: Formal analysis. JO: Resources, Supervision, Project administration. XG: Resources, Supervision, Project administration.

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Correspondence to Xiaoli Gu.

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Ding, C., Xu, C., He, T. et al. Oxygen mass transfer enhancement by activated carbon particles in xylose fermentation media. Bioprocess Biosyst Eng 46, 15–23 (2023). https://doi.org/10.1007/s00449-022-02809-6

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