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Continuous production of monoacylglycerol via glycerolysis of babassu oil by immobilized Burkholderia cepacia lipase in a packed bed reactor

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Abstract

This study investigated the glycerolysis of babassu oil by Burkholderia cepacia lipase immobilized on SiO2–PVA particles in a continuous packed bed reactor. Experiments were conducted in a solvent-free system at 273.15 K either in an inert atmosphere or in the presence of cocoa butter to prevent lipid oxidation. The reactor (15 × 55 mm) was run at a fixed space time of 9.8 h using different molar ratios of babassu oil to glycerol (1:3, 1:6, 1:9, 1:12, and 1:15) to assess the effects of reactant molar ratio on monoacylglycerol productivity and selectivity. Nitrogen atmosphere and cocoa butter were equally effective in inhibiting lipid oxidation, indicating that addition of cocoa butter to glycerolysis reactions may be an interesting cost-reduction strategy. An oil/glycerol molar ratio of 1:9 resulted in the highest productivity (52.3 ± 2.9 mg g−1 h−1) and selectivity (31.5 ± 1.8%). Residence time distribution data were fitted to an axial dispersion model for closed-vessel boundary conditions, giving a mass transfer coefficient (kc) of 3.4229 × 10−6 m s−1. A kinetic model based on elementary steps of the studied reaction was written in Scilab and compared with experimental data, providing standard deviations in the range of 5.5–7.5%.

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Acknowledgements

This study was supported by the São Paulo State Research Foundation (FAPESP, grant nos. 2016/17833-3 and 2016/10636-8), the Brazilian National Council for Scientific and Technological Development (CNPq, process no. 433248/2018-1), and the Brazilian Federal Agency for Support and Evaluation of Graduate Education (CAPES, Finance Code 001).

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Authors and Affiliations

  1. Department of Chemical Engineering, School of Engineering of Lorena, University of São Paulo (USP), 12.602-810, Lorena, SP, Brazil

    Renata N. Vilas Bôas, Rosemar Lima, Mateus V. C. Silva, Larissa Freitas, Leandro G. Aguiar & Heizir F. de Castro

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  1. Renata N. Vilas Bôas
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  2. Rosemar Lima
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  3. Mateus V. C. Silva
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Correspondence to Renata N. Vilas Bôas.

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Vilas Bôas, R.N., Lima, R., Silva, M.V.C. et al. Continuous production of monoacylglycerol via glycerolysis of babassu oil by immobilized Burkholderia cepacia lipase in a packed bed reactor. Bioprocess Biosyst Eng 44, 2205–2215 (2021). https://doi.org/10.1007/s00449-021-02596-6

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