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Improving the biocatalytic performance of co-immobilized cells harboring nitrilase via addition of silica and calcium carbonate

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Abstract

To improve nicotinic acid (NA) yield and meet industrial application requirements of sodium alginate-polyvinyl alcohol (SA-PVA) immobilized cells of Pseudomonas putida mut-D3 harboring nitrilase, inorganic materials were added to the SA-PVA immobilized cells to improve mechanical strength and mass transfer performance. The concentrations of inorganic materials were optimized to be 2.0% silica and 0.6% CaCO3. The optimal pH and temperature for SA-PVA immobilized cells and composite immobilized cells were both 8.0 and 45 °C, respectively. The half-lives of composite immobilized cells were 271.48, 150.92, 92.92 and 33.12 h, which were 1.40-, 1.35-, 1.22- and 1.63-fold compared to SA-PVA immobilized cells, respectively. The storage stability of the composite immobilized cells was slightly increased. The composite immobilized cells could convert 14 batches of 3-cyanopyridine with feeding concentration of 250 mM and accumulate 418 g ·L−1 nicotinic acid, while the SA-PVA immobilized cells accumulated 346 g L−1 nicotinic acid.

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Acknowledgements

This work was financially supported by the National Key R & D Program of China (no. 2019YFA0905300), the Western Talents of Shandong Province (no. 2017GRC5217), the National First-class discipline Program of Light Industry Technology and Engineering (no. LITE2018-18), and the Fundamental Research Funds for the Central Universities (no. JUSRP22047).

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Authors and Affiliations

  1. Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Pharmaceutical Sciences, Jiangnan University, Wuxi, 214122, People’s Republic of China

    Shun-Zhi Wang, Zi-Kai Wang, Jin-Song Gong, Ting-Ting Dong & Jin-Song Shi

  2. National Engineering Laboratory for Cereal Fermentation Technology, School of Biotechnology, Jiangnan University, Wuxi, 214122, People’s Republic of China

    Shun-Zhi Wang & Zheng-Hong Xu

  3. Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, 2800, Kgs. Lyngby, Denmark

    Jiufu Qin

  4. Jiangsu Provincial Research Center for Bioactive Product Processing Technology, Jiangnan University, Wuxi, 214122, People’s Republic of China

    Zheng-Hong Xu

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  1. Shun-Zhi Wang
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  2. Zi-Kai Wang
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  3. Jin-Song Gong
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  4. Jiufu Qin
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  5. Ting-Ting Dong
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  6. Zheng-Hong Xu
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  7. Jin-Song Shi
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Correspondence to Jin-Song Gong or Jin-Song Shi.

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Wang, SZ., Wang, ZK., Gong, JS. et al. Improving the biocatalytic performance of co-immobilized cells harboring nitrilase via addition of silica and calcium carbonate. Bioprocess Biosyst Eng 43, 2201–2207 (2020). https://doi.org/10.1007/s00449-020-02405-6

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  • DOI: https://doi.org/10.1007/s00449-020-02405-6

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