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Immobilization of lipase Eversa Transform 2.0 on poly(urea–urethane) nanoparticles obtained using a biopolyol from enzymatic glycerolysis

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Abstract

In this work, the free lipase Eversa® Transform 2.0 was used as a catalyst for enzymatic glycerolysis reaction in a solvent-free system. The product was evaluated by nuclear magnetic resonance (1H NMR) and showed high conversion related to hydroxyl groups. In sequence, the product of the glycerolysis was used as stabilizer and biopolyol for the synthesis of poly(urea–urethane) nanoparticles (PUU NPs) aqueous dispersion by the miniemulsion polymerization technique, without the use of a further surfactant in the system. Reactions resulted in stable dispersions of PUU NPs with an average diameter of 190 nm. After, the formation of the PUU NPs in the presence of concentrated lipase Eversa® Transform 2.0 was studied, aiming the lipase immobilization on the NP surface, and a stable enzymatic derivative with diameters around 231 nm was obtained. The hydrolytic enzymatic activity was determined using ρ-nitrophenyl palmitate (ρ-NPP) and the immobilization was confirmed by morphological analysis using transmission electron microscopy and fluorescence microscopy.

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Acknowledgements

The authors thank Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq—Process: 142143/2014-6) for the financial support; Novozymes® who kindly provided enzymes; Laboratório Central de Microscopia Eletrônica (LCME) of Universidade Federal de Santa Catarina (UFSC) for transmission electron microscopy images; and Laboratório Multiusuário de Estudos em Biologia (LAMEB) of UFSC for fluorescence microscopy analyses.

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  1. Department of Chemical Engineering and Food Engineering, Federal University of Santa Catarina, P.O. Box 476, Florianopolis, SC, 88040-900, Brazil

    Daniela Bresolin, Beatriz Hawerroth, Cristian de Oliveira Romera, Claudia Sayer, Pedro Henrique Hermes de Araújo & Débora de Oliveira

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  1. Daniela Bresolin
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Correspondence to Débora de Oliveira.

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Bresolin, D., Hawerroth, B., de Oliveira Romera, C. et al. Immobilization of lipase Eversa Transform 2.0 on poly(urea–urethane) nanoparticles obtained using a biopolyol from enzymatic glycerolysis. Bioprocess Biosyst Eng 43, 1279–1286 (2020). https://doi.org/10.1007/s00449-020-02324-6

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