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Effects of sugarcane bagasse hydrolysate (SCBH) on cell growth and fatty acid accumulation of heterotrophic Chlorella protothecoides

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Abstract

Microalgal lipid production by Chlorella protothecoides using sugarcane bagasse hydrolysate was investigated in this study. First, maximum glucose and reducing sugar concentrations of 15.2 and 27.0 g/L were obtained in sugarcane bagasse hydrolysate (SCBH), and the effects of different percentages of glucose and xylose on algal cultivation were investigated. Afterwards, SCBH was used as a carbon source for the cultivation of C. protothecoides and higher biomass concentration of 10.7 g/L was achieved. Additionally, a large amount of fatty acids, accounting up to 16.8% of dry weight, were accumulated in C. protothecoides in the nitrogen-limited (0.1–1 mmol/L) culture. Although SCBH inhibited fatty acid accumulation to a certain degree and the inhibition was aggravated by nitrogen starvation, SCBH favored microalgal cell growth and fatty acid production. The present study is of significance for the integration of cost-effective feedstocks production for biodiesel with low-cost SCBH as well as environmentally friendly disposal of lignocellulosic wastes.

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Acknowledgements

This work was funded by the program of Science and Technology of Guangzhou (Grant no. 201704030084) and the SinoPec Technology Development Program (218017-1).

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Authors and Affiliations

  1. School of Food Science and Engineering, South China University of Technology, Guangzhou, 510641, People’s Republic of China

    Jun-hui Chen, Lu Liu & Dong Wei

  2. Institute of Ocean and Earth Sciences (IOES), University of Malaya, 50603, Kuala Lumpur, Malaysia

    Phaik-Eem Lim

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  1. Jun-hui Chen
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Correspondence to Dong Wei.

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Chen, Jh., Liu, L., Lim, PE. et al. Effects of sugarcane bagasse hydrolysate (SCBH) on cell growth and fatty acid accumulation of heterotrophic Chlorella protothecoides. Bioprocess Biosyst Eng 42, 1129–1142 (2019). https://doi.org/10.1007/s00449-019-02110-z

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